Meme Kanseri Proteom Profilinin Yüzey Geliştirilmiş Lazer Desorpsiyon / İyonizasyon Uçuş Kütle Spektrometrisi (SELDI-TOF MS) ile Belirlenmesi

Yasemin BASKIN; Türkan YİĞİTBAŞI; Murat Kemal ATAHAN; Hasan KÜPELİ; Seyran YİĞİT; Ercüment TARCAN

Article Details

Meme Kanseri Proteom Profilinin Yüzey Geliştirilmiş Lazer Desorpsiyon / İyonizasyon Uçuş Kütle Spektrometrisi (SELDI-TOF MS) ile Belirlenmesi

Authors:

Yasemin BASKIN | Türkan YİĞİTBAŞI | Murat Kemal ATAHAN | Hasan KÜPELİ | Seyran YİĞİT | Ercüment TARCAN

Publisher:

Celal Bayar University

Journal:

CBU-SBED: Celal Bayar University-Health Sciences Institute Journal 2015 Vol.2 Issue 4, pp.101-105

Document Type:

Article

Publication Date:

12/2015

Document Language:

Turkish

Document ID (Org No.):

2000708604

Frequency:

Quarterly

eISSN:

2147-9607

Abstract:

 

Objective: Surface-enhanced laser desorption/ionization time-of flight mass spectrometry (SELDI-TOF MS) was used to screen serum proteomic
profiling to identify protein markers for early breast cancer patients.
Methods: We used metal ion affinity chromatography (IMAC30-Cu) for serum proteome fractionation technique and identify differentially
expressed peptides (m/z; P < 0.0001) from benign disease and healthy controls as potential biomarkers of early breast cancer. The mass spectra,
analyzed using ProteinChip Data Manager Software, distinguished between patients and healthy individuals based on k-nearest neighbor genetic
algorithm. We collected 70 serum samples from patients with breast cancer (62 invasive breast carcinoma; 8 ductal carcinoma in situ), 31 bening
disease and 37 healthy individuals.
Results: Forty two peaks showed statistically significantly different intensities between cases and controls (P<0.05); 10 protein peaks were
differentially expressed between the patients and benign disease. These results indicate that there are differences in serum protein expression among
the three different groups of patients.
Conclusion: SELDI-TOF MS could be used to screen blood samples for the early detection of breast cancer patients. Serum proteomic profiling by
SELDI-TOF MS is a novel potential tool for the clinical diagnosis of patients with breast carcinoma.

 

AMAÇ: Yüzey geliştirilmiş lazer desorpsiyon / iyonizasyon (SELDI) Uçuş kütle spektrometrisi (TOF MS) kullanılarak erken evre meme kanseri hastalarında serum proteom profilinde protein markerlar tanımlandı.YÖNTEMLER: Serum proteom fraksiyonlarını ayrımlama için metal iyon kromatografi (IMAC30-Cu) kullanıldı ve farklı şekilde eksprese olan peptitler (m / z; p.

Article Details

Meme Kanseri Proteom Profilinin Yüzey Geliştirilmiş Lazer Desorpsiyon / İyonizasyon Uçuş Kütle Spektrometrisi (SELDI-TOF MS) ile Belirlenmesi

Authors: Yasemin BASKIN | Türkan YİĞİTBAŞI | Murat Kemal ATAHAN | Hasan KÜPELİ | Seyran YİĞİT | Ercüment TARCAN

Publisher: Celal Bayar University

Journal: CBU-SBED: Celal Bayar University-Health Sciences Institute Journal 2015 Vol.2 Issue 4, pp.101-105

Document Type: Article

Publication Date: 12/2015

Document Language: Turkish

Document ID (Org No.): 2000708604

Frequency: Quarterly

eISSN: 2147-9607

Issues

Authors:

Yasemin BASKIN | Türkan YİĞİTBAŞI | Murat Kemal ATAHAN | Hasan KÜPELİ | Seyran YİĞİT | Ercüment TARCAN

Publisher:

Celal Bayar University

Journal:

CBU-SBED: Celal Bayar University-Health Sciences Institute Journal 2015 Vol.2 Issue 4, pp.101-105

Document Type:

Article

Publication Date:

12/2015

Document Language:

Turkish

Document ID (Org No.):

2000708604

Frequency:

Quarterly

eISSN:

2147-9607