Use of Acidic Hydrolysis and Diazo Coupling Reaction for Spectrophotometric Determination of Furosemide in Urine and Pharmaceutical Formulation.

Hawraa Ali; Sumayha muhammed

doi:10.30526/2017.IHSCICONF.1795

Article Details

Use of Acidic Hydrolysis and Diazo Coupling Reaction for Spectrophotometric Determination of Furosemide in Urine and Pharmaceutical Formulation.

Authors:

Hawraa Ali | Sumayha muhammed

Publisher:

University of Baghdad

Journal:

Ibn AL-Haitham Journal For Pure and Applied Sciences Issue Special Issue, pp.221-234

Document Type:

Article

Publication Date:

1/2017

Document Language:

English

Document ID (Org No.):

2001377510

pISSN:

2521-3407

DOI:

10.30526/2017.IHSCICONF.1795

Keywords:

Abstract:

 Simple and rapid spectrophotometric determination of furosemide (FUR) has been investigated .The method is based on acid hydrolysis of FUR to free primary aromatic amine and diazotization followed by coupling with 3, 5 di methyl phenol (3, 5-DMPH) at basic medium. The absorbance was measured at 434 nm, the method was optimized for best condition, and beersâ€™ law is obeyed over the range of 0.4-50 Âµg.mL-1 with molar absorptivity and sandalâ€™s sensitivity 1.3899 x104 L moL-1 .cm-1 and 0.0238x104 Âµg.cm-2 respectively. Analysis of solution containing nineteen different concentrations of FUR gave a correlation coefficient of (0.9999) and limit of detection, limit of quantitation were 0.127, 0.464Âµg.mL-1 respectively. The reaction stoichiometry was evaluated by Jobâ€™s and mole ratio method was found to be 1:1(diazotized FUR: 3, 5-DMPH) .The method was applied in synthetic urine and pharmaceutical formulation. The recovery of FUR in spiked urine was satisfactory resulting in the values of (99Â±3.32) %, the results of the suggested method was compared with available official literature method.

Article Details

Use of Acidic Hydrolysis and Diazo Coupling Reaction for Spectrophotometric Determination of Furosemide in Urine and Pharmaceutical Formulation.

Authors:

Hawraa Ali | Sumayha muhammed

Publisher:

University of Baghdad

Journal:

Ibn AL-Haitham Journal For Pure and Applied Sciences Issue Special Issue, pp.221-234

Document Type:

Article

Publication Date:

1/2017

Document Language:

English

Document ID (Org No.):

2001377510

pISSN:

2521-3407

DOI:

10.30526/2017.IHSCICONF.1795

Furosemide

3,5-Dimethyl phenol Spectrophotometr Diazotization

Issues

Article Details

Use of Acidic Hydrolysis and Diazo Coupling Reaction for Spectrophotometric Determination of Furosemide in Urine and Pharmaceutical Formulation.

Authors: Hawraa Ali | Sumayha muhammed

Publisher: University of Baghdad

Journal: Ibn AL-Haitham Journal For Pure and Applied Sciences Issue Special Issue, pp.221-234

Document Type: Article

Publication Date: 1/2017

Document Language: English

Document ID (Org No.): 2001377510

pISSN: 2521-3407

DOI: 10.30526/2017.IHSCICONF.1795

Furosemide

3,5-Dimethyl phenol Spectrophotometr Diazotization

Issues

Authors:

Hawraa Ali | Sumayha muhammed

Publisher:

University of Baghdad

Journal:

Ibn AL-Haitham Journal For Pure and Applied Sciences Issue Special Issue, pp.221-234

Document Type:

Article

Publication Date:

1/2017

Document Language:

English

Document ID (Org No.):

2001377510

pISSN:

2521-3407

DOI:

10.30526/2017.IHSCICONF.1795