EXPLORING ALKALINE PROTEASE ACTIVITY OF BACILLUS STRAIN ISOLATED FROM SIDON DUMPSITE LEBANON

Zahra Farroukh; Dalia El-Badan; Jamilah Borjac

Article Details

EXPLORING ALKALINE PROTEASE ACTIVITY OF BACILLUS STRAIN ISOLATED FROM SIDON DUMPSITE LEBANON

Authors:

Zahra Farroukh | Dalia El-Badan | Jamilah Borjac

Publisher:

Beirut Arab University Press

Journal:

BAU Journal - Science and Technology Vol.7 Issue 1, pp.0-0

Document Type:

Article

Publication Date:

12/2025

Document Language:

English

Document ID (Org No.):

2001951595

pISSN:

2959-331X

Keywords:

Abstract:

 Proteases are crucial hydrolytic enzymes present in all organisms, performing essential physiological and industrial tasks. This study was conducted to screen and isolate protease-producing bacteria from soil and water samples collected from Sidon dumpsite in Lebanon. Bacterial samples were cultured onto skim milk agar medium to identify the alkaline protease-producing ones. In this study, we report on the partial purification and characterization of one of the isolated alkaline protease-producing bacteria. The bacterium was identified as Bacillus subtilis Z3 based on 16S rRNA sequencing. The molecular weight of the partially purified protease was approximately 55 kDa, estimated by sodium dodecyl sulphate polyacrylamide gel electrophoresis. Enzyme activity was determined under varying conditions of pH, incubation time, temperature, and carbon and nitrogen sources. The maximum protease activity was observed at 37 oC and pH 9.0, which was strongly activated by two metal ions, mainly Ca+2 and Mg+2. Phenylmethylsulphonyl fluoride fully inhibited the enzyme activity, indicating that this protease is a member of the serine protease family. Furthermore, the enzyme exhibited significant stability upon being exposed to different surfactants and solvents. Inclusion of this protease in detergents enhanced their ability to remove blood stains as well as to degrade feathers. Therefore, this isolated bacterial protease could be a valuable option for the detergent industry.

Article Details

EXPLORING ALKALINE PROTEASE ACTIVITY OF BACILLUS STRAIN ISOLATED FROM SIDON DUMPSITE LEBANON

Authors:

Zahra Farroukh | Dalia El-Badan | Jamilah Borjac

Publisher:

Beirut Arab University Press

Journal:

BAU Journal - Science and Technology Vol.7 Issue 1, pp.0-0

Document Type:

Article

Publication Date:

12/2025

Document Language:

English

Document ID (Org No.):

2001951595

pISSN:

2959-331X

Bacillus subtilis

Purification

Skim milk

Optimization

Blood de-staining

Feathers degradation

Issues

Article Details

EXPLORING ALKALINE PROTEASE ACTIVITY OF BACILLUS STRAIN ISOLATED FROM SIDON DUMPSITE LEBANON

Authors: Zahra Farroukh | Dalia El-Badan | Jamilah Borjac

Publisher: Beirut Arab University Press

Journal: BAU Journal - Science and Technology Vol.7 Issue 1, pp.0-0

Document Type: Article

Publication Date: 12/2025

Document Language: English

Document ID (Org No.): 2001951595

pISSN: 2959-331X

Bacillus subtilis

Purification

Skim milk

Optimization

Blood de-staining

Feathers degradation

Issues

Authors:

Zahra Farroukh | Dalia El-Badan | Jamilah Borjac

Publisher:

Beirut Arab University Press

Journal:

BAU Journal - Science and Technology Vol.7 Issue 1, pp.0-0

Document Type:

Article

Publication Date:

12/2025

Document Language:

English

Document ID (Org No.):

2001951595

pISSN:

2959-331X