Linear assembly and 3D networks of peptide modified gold nanoparticles

Şaban KALAY; Clement BLANCHET; Mustafa CULHA

Article Details

Linear assembly and 3D networks of peptide modified gold nanoparticles

Authors:

Şaban KALAY | Clement BLANCHET | Mustafa CULHA

Publisher:

TUBITAK

Journal:

Turkish Journal of Chemistry 2014 Vol.38 Issue 5, pp.686-700

Document Type:

Article

Publication Date:

4/2014

Document Language:

Turkish

Document ID (Org No.):

2000727056

Frequency:

Bimonthly

eISSN:

1303-6130

pISSN:

1300-0527

Abstract:

 

The charge and size of molecules chemically attached to nanoparticles (NPs)  play an important role in their interaction behavior in suspensions. Gold  nanoparticles (AuNPs) were modified systematically with peptides and the  modification was verified with surface-enhanced Raman scattering (SERS). The  behavior of the modified AuNPs in suspension and at the liquid--solid  interface was monitored using small angle X-ray scattering (SAXS), UV/Vis  spectroscopy and dynamic light scattering (DLS) in suspension, and atomic  force microscopy (AFM) at the solid--liquid interface. It was found that  while negatively charged peptide modified AuNPs behave very similar to  citrate reduced AuNPs due to their negatively charged surface, positively  charged peptide modified AuNPs showed significantly different  assembly/aggregation properties in suspension. The formation of linear  assemblies of positively charged peptide (CKRHSKRHRSKRHSKRHSKRHSKR) modified  AuNPs was clearly observed from the AFM analysis of the droplet areas of its  colloidal suspension. The combined analyses of data obtained from the  employed techniques suggest that the positively charged large peptide  modified AuNPs can form linear and 3D-like networks in the suspension. This  study reveals important information regarding the surface property dependent  behavior of NPs that may help in efforts to build higher structures using  NPs as building blocks.

 

The charge and size of molecules chemically attached to nanoparticles (NPs)  play an important role in their interaction behavior in suspensions. Gold  nanoparticles (AuNPs) were modified systematically with peptides and the  modification was verified with surface-enhanced Raman scattering (SERS). The  behavior of the modified AuNPs in suspension and at the liquid--solid  interface was monitored using small angle X-ray scattering (SAXS), UV/Vis  spectroscopy and dynamic light scattering (DLS) in suspension, and atomic  force microscopy (AFM) at the solid--liquid interface. It was found that  while negatively charged peptide modified AuNPs behave very similar to  citrate reduced AuNPs due to their negatively charged surface, positively  charged peptide modified AuNPs showed significantly different  assembly/aggregation properties in suspension. The formation of linear  assemblies of positively charged peptide (CKRHSKRHRSKRHSKRHSKRHSKR) modified  AuNPs was clearly observed from the AFM analysis of the droplet areas of its  colloidal suspension. The combined analyses of data obtained from the  employed techniques suggest that the positively charged large peptide  modified AuNPs can form linear and 3D-like networks in the suspension. This  study reveals important information regarding the surface property dependent  behavior of NPs that may help in efforts to build higher structures using  NPs as building blocks.

Article Details

Linear assembly and 3D networks of peptide modified gold nanoparticles

Authors: Şaban KALAY | Clement BLANCHET | Mustafa CULHA

Publisher: TUBITAK

Journal: Turkish Journal of Chemistry 2014 Vol.38 Issue 5, pp.686-700

Document Type: Article

Publication Date: 4/2014

Document Language: Turkish

Document ID (Org No.): 2000727056

Frequency: Bimonthly

eISSN: 1303-6130

pISSN: 1300-0527

Issues

Authors:

Şaban KALAY | Clement BLANCHET | Mustafa CULHA

Publisher:

TUBITAK

Journal:

Turkish Journal of Chemistry 2014 Vol.38 Issue 5, pp.686-700

Document Type:

Article

Publication Date:

4/2014

Document Language:

Turkish

Document ID (Org No.):

2000727056

Frequency:

Bimonthly

eISSN:

1303-6130

pISSN:

1300-0527