Use of poly HEMA-MAH -Cu2+ Microbeads for α-Amylase Immobilization

Ayşegül AYLA; Zübeyde BAYSAL

Article Details

Use of poly HEMA-MAH -Cu2+ Microbeads for α-Amylase Immobilization

Authors:

Ayşegül AYLA | Zübeyde BAYSAL

Publisher:

Hacettepe University

Journal:

Hacettepe Journal of Biology and Chemistry 2014 Vol.42 Issue 3, pp.323-330

Document Type:

Article

Publication Date:

8/2014

Document Language:

English

Document ID (Org No.):

2000449123

Frequency:

Quarterly

eISSN:

2687-475X

pISSN:

2687-475X

Abstract:

 

In this article poly 2-hydroxyethyl methacrylate-N-methacryloyl- l -histidin methylester  [P HEMA-MAH ] microbeads were prepared by suspension polymerization of 2-hydroxyethyl methacrylate  HEMA  and N-methacryloyl- l -histidine methyl ester  MAH . Some properties of the p HEMA-MAH  microbeads were determined by using scanning electron microscopy  SEM  and swelling tests. Cu2+ ions were chelated on the p HEMA-MAH  microbeads, then these microbeads were used in the immobilization of α-amylase in batch system. The maximum α-amylase adsorption capacity of the p HEMA-MAH -Cu2+ beads was found to be 14.88 mg/g at pH 6.0. The optimum temperatures for the immobilized and free enzyme were determined to be 30oC and 25 oC, respectively. Kinetic parameters  Km, Vmax  of the immobilized enzyme were also determined and compared with those of free enzyme. Reuse studies showed that the immobilized enzyme could reuse six times while retaining 76% of its activity.

 

S Bu çalışmada poli 2-hidroksietil metakrilat-N-metakriloil- L -histidin metilester [P HEMA-MAH ] mikroküreler süspansiyon polimerizasyon yöntemiyle 2-hidroksietil metakrilat  HEMA  ve N-metakriloil- L -histidin metil ester  MAH  ile hazırlandı. P HEMA-MAH  mikrokürelerin bazı özellikleri taramalı elektron mikroskobu  SEM  ve şişme testleriyle belirlendi. P HEMA-MAH  mikrokürelere Cu2+ bağlandıktan sonra bu mikroküreler α-amilaz immobilizasyonu için kullanıldı. P HEMA-MAH -Cu2+ mikrokürelerin maksimum α-amilaz adsorpsiyon kapasitesi pH 6.0’da 14.88 mg/g olarak bulundu. İmmobilize ve serbest enzim için optimum sıcaklık sırasıyla 30ºC ve 25ºC olarak belirlendi. İmmobilize enzim için kinetik parametreler de  Km, Vmax  belirlenerek serbest enzim ile karşılaştırıldı. Tekrarlılık çalışmaları, immobilize enzimin orijinal aktivitesinin %76’sını kaybetmeden altı kez tekrar kulanılabilirliğini gösterdi.

Article Details

Use of poly HEMA-MAH -Cu2+ Microbeads for α-Amylase Immobilization

Authors: Ayşegül AYLA | Zübeyde BAYSAL

Publisher: Hacettepe University

Journal: Hacettepe Journal of Biology and Chemistry 2014 Vol.42 Issue 3, pp.323-330

Document Type: Article

Publication Date: 8/2014

Document Language: English

Document ID (Org No.): 2000449123

Frequency: Quarterly

eISSN: 2687-475X

pISSN: 2687-475X

Issues

Authors:

Ayşegül AYLA | Zübeyde BAYSAL

Publisher:

Hacettepe University

Journal:

Hacettepe Journal of Biology and Chemistry 2014 Vol.42 Issue 3, pp.323-330

Document Type:

Article

Publication Date:

8/2014

Document Language:

English

Document ID (Org No.):

2000449123

Frequency:

Quarterly

eISSN:

2687-475X

pISSN:

2687-475X