Investigation of using cave bacteria in the production of self-healing mortars

Nihal Doğruöz Güngör

Article Details

Investigation of using cave bacteria in the production of self-healing mortars

Authors:

Nihal Doğruöz Güngör

Publisher:

Yildiz Technical University

Main Topics:

Biochemistry and Cell Biology (Other)

Journal:

Sigma Journal of Engineering and Natural Sciences Vol.42 Issue 6, pp.1712-1728

Document Type:

Article

Publication Date:

9/2024

Document Language:

English

Document ID (Org No.):

2001333109

pISSN:

1304-7191

Keywords:

Abstract:

 Self-healing concrete is a type of concrete formed by delayed or secondary hydration of cement and its particles with water in the matrix leaking through cracks, and calcium hydroxide and carbon dioxide on the crack surface react with calcium carbonate to provide protection against leakage through cracks. In recent studies, it has been observed that bacteria can self-repair with the production of calcium carbonate-based minerals and this repair provides protection against leakage, pore permeability and protection with increased strength and durability by reducing the infiltration of harmful substances into the internal structure. In this study, 32 different batches of samples were produced by using Bacillus thuringiensis, Pseudomonas putida and Sphingomonas mucosissima bacteria in mortar mixtures prepared by traditional methods with 2 different watercement ratios and 3 different mineral additives. The bacteria used are cave isolates that provide calcium carbonate formation, and these isolates were multiplied in the appropriate growth culture and then added to the prepared mortar samples together with the mixture water. The specimens were cured with tap water for 28 days and then subjected to suction, ultrasonic, flexural and compressive strength tests. The results of the tests revealed a reduction in the porous structure of the specimens containing bacteria, resulting in lower water absorption rates compared to the reference specimens. It was observed that most CaCO3 was produced by Pseudomonas putida bacteria. However, there was no significant difference in compressive and flexural strengths compared to the reference specimens.

 Self-healing concrete is a type of concrete formed by delayed or secondary hydration of cement and its particles with water in the matrix leaking through cracks, and calcium hydroxide and carbon dioxide on the crack surface react with calcium carbonate to provide protection against leakage through cracks. In recent studies, it has been observed that bacteria can self-repair with the production of calcium carbonate-based minerals and this repair provides protection against leakage, pore permeability and protection with increased strength and durability by reducing the infiltration of harmful substances into the internal structure. In this study, 32 different batches of samples were produced by using Bacillus thuringiensis, Pseudomonas putida and Sphingomonas mucosissima bacteria in mortar mixtures prepared by traditional methods with 2 different watercement ratios and 3 different mineral additives. The bacteria used are cave isolates that provide calcium carbonate formation, and these isolates were multiplied in the appropriate growth culture and then added to the prepared mortar samples together with the mixture water. The specimens were cured with tap water for 28 days and then subjected to suction, ultrasonic, flexural and compressive strength tests. The results of the tests revealed a reduction in the porous structure of the specimens containing bacteria, resulting in lower water absorption rates compared to the reference specimens. It was observed that most CaCO3 was produced by Pseudomonas putida bacteria. However, there was no significant difference in compressive and flexural strengths compared to the reference specimens.

 Self-healing concrete is a type of concrete formed by delayed or secondary hydration of cement and its particles with water in the matrix leaking through cracks, and calcium hydroxide and carbon dioxide on the crack surface react with calcium carbonate to provide protection against leakage through cracks. In recent studies, it has been observed that bacteria can self-repair with the production of calcium carbonate-based minerals and this repair provides protection against leakage, pore permeability and protection with increased strength and durability by reducing the infiltration of harmful substances into the internal structure. In this study, 32 different batches of samples were produced by using Bacillus thuringiensis, Pseudomonas putida and Sphingomonas mucosissima bacteria in mortar mixtures prepared by traditional methods with 2 different watercement ratios and 3 different mineral additives. The bacteria used are cave isolates that provide calcium carbonate formation, and these isolates were multiplied in the appropriate growth culture and then added to the prepared mortar samples together with the mixture water. The specimens were cured with tap water for 28 days and then subjected to suction, ultrasonic, flexural and compressive strength tests. The results of the tests revealed a reduction in the porous structure of the specimens containing bacteria, resulting in lower water absorption rates compared to the reference specimens. It was observed that most CaCO3 was produced by Pseudomonas putida bacteria. However, there was no significant difference in compressive and flexural strengths compared to the reference specimens.

 Self-healing concrete is a type of concrete formed by delayed or secondary hydration of cement and its particles with water in the matrix leaking through cracks, and calcium hydroxide and carbon dioxide on the crack surface react with calcium carbonate to provide protection against leakage through cracks. In recent studies, it has been observed that bacteria can self-repair with the production of calcium carbonate-based minerals and this repair provides protection against leakage, pore permeability and protection with increased strength and durability by reducing the infiltration of harmful substances into the internal structure. In this study, 32 different batches of samples were produced by using Bacillus thuringiensis, Pseudomonas putida and Sphingomonas mucosissima bacteria in mortar mixtures prepared by traditional methods with 2 different watercement ratios and 3 different mineral additives. The bacteria used are cave isolates that provide calcium carbonate formation, and these isolates were multiplied in the appropriate growth culture and then added to the prepared mortar samples together with the mixture water. The specimens were cured with tap water for 28 days and then subjected to suction, ultrasonic, flexural and compressive strength tests. The results of the tests revealed a reduction in the porous structure of the specimens containing bacteria, resulting in lower water absorption rates compared to the reference specimens. It was observed that most CaCO3 was produced by Pseudomonas putida bacteria. However, there was no significant difference in compressive and flexural strengths compared to the reference specimens.

Article Details

Investigation of using cave bacteria in the production of self-healing mortars

Authors:

Nihal Doğruöz Güngör

Publisher:

Yildiz Technical University

Main Topics:

Biochemistry and Cell Biology (Other)

Journal:

Sigma Journal of Engineering and Natural Sciences Vol.42 Issue 6, pp.1712-1728

Document Type:

Article

Publication Date:

9/2024

Document Language:

English

Document ID (Org No.):

2001333109

pISSN:

1304-7191

Autonomous Healing

Biomineralization

Biorepair

Microbial CaCO3

Self-healing

Issues

Article Details

Investigation of using cave bacteria in the production of self-healing mortars

Authors: Nihal Doğruöz Güngör

Publisher: Yildiz Technical University

Main Topics: Biochemistry and Cell Biology (Other)

Journal: Sigma Journal of Engineering and Natural Sciences Vol.42 Issue 6, pp.1712-1728

Document Type: Article

Publication Date: 9/2024

Document Language: English

Document ID (Org No.): 2001333109

pISSN: 1304-7191

Autonomous Healing

Biomineralization

Biorepair

Microbial CaCO3

Self-healing

Issues

Authors:

Nihal Doğruöz Güngör

Publisher:

Yildiz Technical University

Main Topics:

Biochemistry and Cell Biology (Other)

Journal:

Sigma Journal of Engineering and Natural Sciences Vol.42 Issue 6, pp.1712-1728

Document Type:

Article

Publication Date:

9/2024

Document Language:

English

Document ID (Org No.):

2001333109

pISSN:

1304-7191