Evaluation of in vitro Antioxidant, Antimicrobial and Cytotoxic Activities of Crude Ethanol Extract and Fractions of Achillea sintenisii Hub. Mor.

Nuraniye Eruygur; Mehmet Ataş; Mehmet Tekin; Ozge Cevik

Article Details

Evaluation of in vitro Antioxidant, Antimicrobial and Cytotoxic Activities of Crude Ethanol Extract and Fractions of Achillea sintenisii Hub. Mor.

Authors:

Nuraniye Eruygur | Mehmet Ataş | Mehmet Tekin | Ozge Cevik

Publisher:

Marmara University

Main Topics:

Pharmacognosy

Journal:

Clinical and Experimental Health Sciences Vol.13 Issue 3, pp.517-524

Document Type:

Article

Publication Date:

11/2023

Document Language:

English

Document ID (Org No.):

2001298170

pISSN:

2459-1459

Keywords:

Abstract:

 Objective: The Achillea species have been used to treat various ailments due to its anti-inflammatory, hemostatic, spasmolytic and cholagogue effects in the Turkish traditional medicine. However, there is no biological activity studies on some Achillea species except for the well-knowns. This work aimed to determine the antioxidant, antimicrobial and cytotoxic activities of the crude ethanolic extracts and fractions of Achillea sintenisii using in vitro methods. Methods: The antioxidant activity was investigated by DPPH 1,1-diphenyl- 2-picrylhydrazyl, ABTS 2,2-azino-bis 3-ethylbenzothiazoline-6-sulphonic acid radical scavenging, total phenol and total flavonoid content, and iron chelating methods. Antimicrobial activity evaluated by micro-plate dilution method against five test organisms. Cytotoxicity was determined by MTT method using MCF-7 breast cancer cell line and PC-3 prostate cancer cell line. Apoptosis was also measured by AOEB staining. Results: The n-Hexane fractions showed the highest antimicrobial and cytotoxic activities, respectively. Administration of the extracts on the cancer cells showed a concentration dependent inhibition on cell proliferation. The anti-proliferation effect could be via apoptosis and associated with the cell death. Conclusion: The results showed that the extracts demonstrated antioxidant, antimicrobial, and cytotoxic activity, also supports the claims of traditional usage.

 Objective: The Achillea species have been used to treat various ailments due to its anti-inflammatory, hemostatic, spasmolytic and cholagogue effects in the Turkish traditional medicine. However, there is no biological activity studies on some Achillea species except for the well-knowns. This work aimed to determine the antioxidant, antimicrobial and cytotoxic activities of the crude ethanolic extracts and fractions of Achillea sintenisii using in vitro methods. Methods: The antioxidant activity was investigated by DPPH 1,1-diphenyl- 2-picrylhydrazyl, ABTS 2,2-azino-bis 3-ethylbenzothiazoline-6-sulphonic acid radical scavenging, total phenol and total flavonoid content, and iron chelating methods. Antimicrobial activity evaluated by micro-plate dilution method against five test organisms. Cytotoxicity was determined by MTT method using MCF-7 breast cancer cell line and PC-3 prostate cancer cell line. Apoptosis was also measured by AOEB staining. Results: The n-Hexane fractions showed the highest antimicrobial and cytotoxic activities, respectively. Administration of the extracts on the cancer cells showed a concentration dependent inhibition on cell proliferation. The anti-proliferation effect could be via apoptosis and associated with the cell death. Conclusion: The results showed that the extracts demonstrated antioxidant, antimicrobial, and cytotoxic activity, also supports the claims of traditional usage.

 Objective: The Achillea species have been used to treat various ailments due to its anti-inflammatory, hemostatic, spasmolytic and cholagogue effects in the Turkish traditional medicine. However, there is no biological activity studies on some Achillea species except for the well-knowns. This work aimed to determine the antioxidant, antimicrobial and cytotoxic activities of the crude ethanolic extracts and fractions of Achillea sintenisii using in vitro methods. Methods: The antioxidant activity was investigated by DPPH 1,1-diphenyl- 2-picrylhydrazyl, ABTS 2,2-azino-bis 3-ethylbenzothiazoline-6-sulphonic acid radical scavenging, total phenol and total flavonoid content, and iron chelating methods. Antimicrobial activity evaluated by micro-plate dilution method against five test organisms. Cytotoxicity was determined by MTT method using MCF-7 breast cancer cell line and PC-3 prostate cancer cell line. Apoptosis was also measured by AOEB staining. Results: The n-Hexane fractions showed the highest antimicrobial and cytotoxic activities, respectively. Administration of the extracts on the cancer cells showed a concentration dependent inhibition on cell proliferation. The anti-proliferation effect could be via apoptosis and associated with the cell death. Conclusion: The results showed that the extracts demonstrated antioxidant, antimicrobial, and cytotoxic activity, also supports the claims of traditional usage.

 Objective: The Achillea species have been used to treat various ailments due to its anti-inflammatory, hemostatic, spasmolytic and cholagogue effects in the Turkish traditional medicine. However, there is no biological activity studies on some Achillea species except for the well-knowns. This work aimed to determine the antioxidant, antimicrobial and cytotoxic activities of the crude ethanolic extracts and fractions of Achillea sintenisii using in vitro methods. Methods: The antioxidant activity was investigated by DPPH 1,1-diphenyl- 2-picrylhydrazyl, ABTS 2,2-azino-bis 3-ethylbenzothiazoline-6-sulphonic acid radical scavenging, total phenol and total flavonoid content, and iron chelating methods. Antimicrobial activity evaluated by micro-plate dilution method against five test organisms. Cytotoxicity was determined by MTT method using MCF-7 breast cancer cell line and PC-3 prostate cancer cell line. Apoptosis was also measured by AOEB staining. Results: The n-Hexane fractions showed the highest antimicrobial and cytotoxic activities, respectively. Administration of the extracts on the cancer cells showed a concentration dependent inhibition on cell proliferation. The anti-proliferation effect could be via apoptosis and associated with the cell death. Conclusion: The results showed that the extracts demonstrated antioxidant, antimicrobial, and cytotoxic activity, also supports the claims of traditional usage.

Article Details

Evaluation of in vitro Antioxidant, Antimicrobial and Cytotoxic Activities of Crude Ethanol Extract and Fractions of Achillea sintenisii Hub. Mor.

Authors:

Nuraniye Eruygur | Mehmet Ataş | Mehmet Tekin | Ozge Cevik

Publisher:

Marmara University

Main Topics:

Pharmacognosy

Journal:

Clinical and Experimental Health Sciences Vol.13 Issue 3, pp.517-524

Document Type:

Article

Publication Date:

11/2023

Document Language:

English

Document ID (Org No.):

2001298170

pISSN:

2459-1459

Achillea sintenisii

antioxidant;

microdilution

cytotoxicity

Issues

Article Details

Evaluation of in vitro Antioxidant, Antimicrobial and Cytotoxic Activities of Crude Ethanol Extract and Fractions of Achillea sintenisii Hub. Mor.

Authors: Nuraniye Eruygur | Mehmet Ataş | Mehmet Tekin | Ozge Cevik

Publisher: Marmara University

Main Topics: Pharmacognosy

Journal: Clinical and Experimental Health Sciences Vol.13 Issue 3, pp.517-524

Document Type: Article

Publication Date: 11/2023

Document Language: English

Document ID (Org No.): 2001298170

pISSN: 2459-1459

Achillea sintenisii

antioxidant;

microdilution

cytotoxicity

Issues

Authors:

Nuraniye Eruygur | Mehmet Ataş | Mehmet Tekin | Ozge Cevik

Publisher:

Marmara University

Main Topics:

Pharmacognosy

Journal:

Clinical and Experimental Health Sciences Vol.13 Issue 3, pp.517-524

Document Type:

Article

Publication Date:

11/2023

Document Language:

English

Document ID (Org No.):

2001298170

pISSN:

2459-1459