DEVELOPMENT OF A FAST LIQUID CHROMATOGRAPHY METHOD WITH A CHEMOMETRIC APPROACH BASED ON BOX-BEHNKEN DESIGN FOR THE DETERMINATION OF ANTIDEPRESSANTS IN PHARMACEUTICAL FORMULATIONS

Sercan Yıldırım; Tuğçe Özyiğit

Article Details

DEVELOPMENT OF A FAST LIQUID CHROMATOGRAPHY METHOD WITH A CHEMOMETRIC APPROACH BASED ON BOX-BEHNKEN DESIGN FOR THE DETERMINATION OF ANTIDEPRESSANTS IN PHARMACEUTICAL FORMULATIONS

Authors:

Sercan Yıldırım | Tuğçe Özyiğit

Publisher:

Ankara University

Main Topics:

Eczacılıkta Analitik Kimya

Journal:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 2, pp.597-607

Document Type:

Article

Publication Date:

5/2024

Document Language:

English

Document ID (Org No.):

2001311410

pISSN:

1015-3918

Keywords:

Abstract:

 Objective: The objective of this work was to develop a liquid chromatographic method for the quantification of antidepressants, namely duloxetine DXN, fluoxetine FXN, citalopram CIT, paroxetine PXN, and sertraline SRN, by a chemometric approach based on Box-Behnken design. Material and Method: After initial experiments to determine significant parameters, a Box-Behnken design consisting of 17 experiment sets was carried out. All separations were conducted using an Agilent Poroshell 120 EC-C18 analytical column 75 mm  4.6 mm  2.7 µm. Result and Discussion: The optimum levels of pH, acetonitrile ratio, and flow rate were determined with the desirability function as 2.7, 38, and 1.1 mlmin, respectively. The differences 8 between predicted optimum responses and experimentally obtained results proved the models suitability. Limits of detection and limits of quantification values were in the ranges of 0.17-0.29 µgml and 0.53-0.89 µgml, respectively. The feasibility of the technique was proven by analyzing PXN and DXN formulations.

 Objective: The objective of this work was to develop a liquid chromatographic method for the quantification of antidepressants, namely duloxetine DXN, fluoxetine FXN, citalopram CIT, paroxetine PXN, and sertraline SRN, by a chemometric approach based on Box-Behnken design. Material and Method: After initial experiments to determine significant parameters, a Box-Behnken design consisting of 17 experiment sets was carried out. All separations were conducted using an Agilent Poroshell 120 EC-C18 analytical column 75 mm  4.6 mm  2.7 µm. Result and Discussion: The optimum levels of pH, acetonitrile ratio, and flow rate were determined with the desirability function as 2.7, 38, and 1.1 mlmin, respectively. The differences 8 between predicted optimum responses and experimentally obtained results proved the models suitability. Limits of detection and limits of quantification values were in the ranges of 0.17-0.29 µgml and 0.53-0.89 µgml, respectively. The feasibility of the technique was proven by analyzing PXN and DXN formulations.

 Objective: The objective of this work was to develop a liquid chromatographic method for the quantification of antidepressants, namely duloxetine DXN, fluoxetine FXN, citalopram CIT, paroxetine PXN, and sertraline SRN, by a chemometric approach based on Box-Behnken design. Material and Method: After initial experiments to determine significant parameters, a Box-Behnken design consisting of 17 experiment sets was carried out. All separations were conducted using an Agilent Poroshell 120 EC-C18 analytical column 75 mm  4.6 mm  2.7 µm. Result and Discussion: The optimum levels of pH, acetonitrile ratio, and flow rate were determined with the desirability function as 2.7, 38, and 1.1 mlmin, respectively. The differences 8 between predicted optimum responses and experimentally obtained results proved the models suitability. Limits of detection and limits of quantification values were in the ranges of 0.17-0.29 µgml and 0.53-0.89 µgml, respectively. The feasibility of the technique was proven by analyzing PXN and DXN formulations.

 Amaç: Bu çalışmanın amacı, duloksetin DXN, fluoksetin FXN, sitalopram CIT, paroksetin PXN ve sertralin SRN adlı antidepresanların tayini için Box-Behnken tasarımına dayalı kemometrik bir yaklaşımla sıvı kromatografik bir yöntem geliştirmektir. Gereç ve Yöntem: Önemli parametreleri belirlemek için yapılan ilk deneylerden sonra, 17 deney setinden oluşan bir Box-Behnken tasarımı gerçekleştirilmiştir. Tüm ayırma işlemleri bir Agilent Poroshell 120 EC-C18 analitik kolon 75 mm  4.6 mm  2.7 µm kullanılarak gerçekleştirilmiştir. Sonuç ve Tartışma: pH, asetonitril oranı ve akış hızının optimum seviyeleri, arzu edilebilirlik fonksiyonu ile sırasıyla 2.7, 38.2 ve 1.1 mldak olarak belirlenmiştir. Tahmin edilen optimum yanıtlar ile deneysel olarak elde edilen sonuçlar arasındaki farklar 8 modelin uygunluğunu kanıtlamıştır. Tespit ve tayin limitleri sırasıyla 0.17-0.29 µgml ve 0.53-0.89 µgml aralığındadır. Yöntemin uygulanabilirliği, PXN ve DXNnin formülasyonlarının analiz edilmesiyle kanıtlanmıştır.

Article Details

DEVELOPMENT OF A FAST LIQUID CHROMATOGRAPHY METHOD WITH A CHEMOMETRIC APPROACH BASED ON BOX-BEHNKEN DESIGN FOR THE DETERMINATION OF ANTIDEPRESSANTS IN PHARMACEUTICAL FORMULATIONS

Authors:

Sercan Yıldırım | Tuğçe Özyiğit

Publisher:

Ankara University

Main Topics:

Eczacılıkta Analitik Kimya

Journal:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 2, pp.597-607

Document Type:

Article

Publication Date:

5/2024

Document Language:

English

Document ID (Org No.):

2001311410

pISSN:

1015-3918

Antidepressants

design of experiments

liquid chromatographyAntidepresanlar

deneysel tasarım

sıvı kromatografisi

Issues

Article Details

DEVELOPMENT OF A FAST LIQUID CHROMATOGRAPHY METHOD WITH A CHEMOMETRIC APPROACH BASED ON BOX-BEHNKEN DESIGN FOR THE DETERMINATION OF ANTIDEPRESSANTS IN PHARMACEUTICAL FORMULATIONS

Authors: Sercan Yıldırım | Tuğçe Özyiğit

Publisher: Ankara University

Main Topics: Eczacılıkta Analitik Kimya

Journal: Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 2, pp.597-607

Document Type: Article

Publication Date: 5/2024

Document Language: English

Document ID (Org No.): 2001311410

pISSN: 1015-3918

Antidepressants

design of experiments

liquid chromatographyAntidepresanlar

deneysel tasarım

sıvı kromatografisi

Issues

Authors:

Sercan Yıldırım | Tuğçe Özyiğit

Publisher:

Ankara University

Main Topics:

Eczacılıkta Analitik Kimya

Journal:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 2, pp.597-607

Document Type:

Article

Publication Date:

5/2024

Document Language:

English

Document ID (Org No.):

2001311410

pISSN:

1015-3918