Diversity of endophytic fungi from various Aegean and Mediterranean orchids (saleps)

Yüksel GEZGİN; Rengin ELTEM

Article Details

Diversity of endophytic fungi from various Aegean and Mediterranean orchids (saleps)

Authors:

Yüksel GEZGİN | Rengin ELTEM

Publisher:

TUBITAK

Journal:

Turkish Journal of Botany 2009 Vol.33 Issue 6, pp.439-445

Document Type:

Article

Publication Date:

5/2009

Document Language:

Turkish

Document ID (Org No.):

2000723420

Frequency:

Bimonthly

eISSN:

1303-6106

pISSN:

1300-008X

Abstract:

 

The diversity and host specificity of endophytic and Rhizoctonia-like fungi were investigated in orchids from the Aegean and Mediterranean regions. Endophytic fungi from various Aegean and Mediterranean orchids (Anacamptis pyramidalis (L.) L.C.M.Richard, Orchis sancta L., Ophrys fusca Link., and Serapias vomeracea subsp. orientalis Greuter) were isolated and identified partially. Surface sterilisation of roots and tubers was carried out in laminar airflow under aseptic conditions. Several modified methods for the isolation of symbiotic fungi from orchid roots and tubers were used. Most of the orchid endophytes isolated was found to be Fusarium Link ex Fr. spp. A total of 47 isolates, having genus characterisations as 44 (94%) isolates belonging to the genus Fusarium, 2 (4%) isolates belonging to the Rhizoctonia DC. ex Fr.-like fungi, and 1 (2%) isolate belonging to the genus Papulaspora Preuss, were found from the orchid root and tubers. Endophytic Fusarium spp. were isolated from Aegean and Mediterranean orchids Anacamptis pyramidalis, Orchis sancta, Ophrys fusca, and Serapias vomeracea subsp. orientalis. Rhizoctonia-like fungi were only isolated from Orchis sancta whereas Papulaspora sp. was only isolated from Anacamptis pyramidalis.

 

The diversity and host specificity of endophytic and Rhizoctonia-like fungi were investigated in orchids from the Aegean and Mediterranean regions. Endophytic fungi from various Aegean and Mediterranean orchids (Anacamptis pyramidalis (L.) L.C.M.Richard, Orchis sancta L., Ophrys fusca Link., and Serapias vomeracea subsp. orientalis Greuter) were isolated and identified partially. Surface sterilisation of roots and tubers was carried out in laminar airflow under aseptic conditions. Several modified methods for the isolation of symbiotic fungi from orchid roots and tubers were used. Most of the orchid endophytes isolated was found to be Fusarium Link ex Fr. spp. A total of 47 isolates, having genus characterisations as 44 (94%) isolates belonging to the genus Fusarium, 2 (4%) isolates belonging to the Rhizoctonia DC. ex Fr.-like fungi, and 1 (2%) isolate belonging to the genus Papulaspora Preuss, were found from the orchid root and tubers. Endophytic Fusarium spp. were isolated from Aegean and Mediterranean orchids Anacamptis pyramidalis, Orchis sancta, Ophrys fusca, and Serapias vomeracea subsp. orientalis. Rhizoctonia-like fungi were only isolated from Orchis sancta whereas Papulaspora sp. was only isolated from Anacamptis pyramidalis.

Article Details

Diversity of endophytic fungi from various Aegean and Mediterranean orchids (saleps)

Authors: Yüksel GEZGİN | Rengin ELTEM

Publisher: TUBITAK

Journal: Turkish Journal of Botany 2009 Vol.33 Issue 6, pp.439-445

Document Type: Article

Publication Date: 5/2009

Document Language: Turkish

Document ID (Org No.): 2000723420

Frequency: Bimonthly

eISSN: 1303-6106

pISSN: 1300-008X

Issues

Authors:

Yüksel GEZGİN | Rengin ELTEM

Publisher:

TUBITAK

Journal:

Turkish Journal of Botany 2009 Vol.33 Issue 6, pp.439-445

Document Type:

Article

Publication Date:

5/2009

Document Language:

Turkish

Document ID (Org No.):

2000723420

Frequency:

Bimonthly

eISSN:

1303-6106

pISSN:

1300-008X