Simultaneous Determination of Irbesartan and Hydrochlorothiazide in Tablets by CE-DAD

Arin Gül DAL; Sema KOYUTÜRK

Article Details

Simultaneous Determination of Irbesartan and Hydrochlorothiazide in Tablets by CE-DAD

Authors:

Arin Gül DAL | Sema KOYUTÜRK

Publisher:

Hacettepe University

Journal:

Hacettepe Journal of Biology and Chemistry 2015 Vol.43 Issue 3, pp.145-152

Document Type:

Article

Publication Date:

8/2015

Document Language:

English

Document ID (Org No.):

2000448993

Frequency:

Quarterly

eISSN:

2687-475X

pISSN:

2687-475X

Abstract:

 

Acapillary electrophoretic method was developed and validated for the simultaneous determination of irbesartan and hydrochlorothiazide in tablets. The run buffer was used 10 mM borate buffer containing 10% methanol  pH 9.0 . Compounds were separated less than 6 minutes. The limit of quantification  LOQ  values were 3.370×10-7 M  0.144 μg/mL  and 3.140×10-7 M  0.094 μg/mL  for irbesartan and hydrochlorothiazide, respectively. The method was applied successfully to both irbesartan and irbesartan/hydrochlorothiazide combi- ned tablets with good recoveries almost 100%.

 

İ rbesartan ve hidroklorotiazidin tabletlerde eş zamanlı analizi için bir elektroforetik yöntem geliştirilmiştir. %10 metanol içeren 10 mM borat  pH 9.0  çalışma tamponu olarak kullanılmıştır. Bileşikler 6 dakikadan daha düşük sürede ayrılmıştır. Tayin sınırı  LOQ  değerleri irbesartan için 3.370×10-7 M  0.144 µg/mL  ve hidroklorotiazid için 3.140×10-7 M  0.094 µg/mL ’dir. Yöntem, irbesartan ve irbesartan/hidroklorotiazidi birlikte içeren tabletlere yaklaşık %100 gibi bir geri kazanımla başarılı bir şekilde uygulanmıştır.

Article Details

Simultaneous Determination of Irbesartan and Hydrochlorothiazide in Tablets by CE-DAD

Authors: Arin Gül DAL | Sema KOYUTÜRK

Publisher: Hacettepe University

Journal: Hacettepe Journal of Biology and Chemistry 2015 Vol.43 Issue 3, pp.145-152

Document Type: Article

Publication Date: 8/2015

Document Language: English

Document ID (Org No.): 2000448993

Frequency: Quarterly

eISSN: 2687-475X

pISSN: 2687-475X

Issues

Authors:

Arin Gül DAL | Sema KOYUTÜRK

Publisher:

Hacettepe University

Journal:

Hacettepe Journal of Biology and Chemistry 2015 Vol.43 Issue 3, pp.145-152

Document Type:

Article

Publication Date:

8/2015

Document Language:

English

Document ID (Org No.):

2000448993

Frequency:

Quarterly

eISSN:

2687-475X

pISSN:

2687-475X