Ciprofloxacin Resistance Due to gyr A Mutation in Pseudomonas Aeruginosa Isolates at Zagazig University Hospitals

Hanaa M. El Maghraby; Rehab A. Rabie

doi:10.12816/0053607

Article Details

Ciprofloxacin Resistance Due to gyr A Mutation in Pseudomonas Aeruginosa Isolates at Zagazig University Hospitals

Authors:

Hanaa M. El Maghraby | Rehab A. Rabie

Publisher:

The Egyptian Society of Medical Microbiology

Main Topics:

Medicine

Journal:

The Egyptian Journal of Medical Microbiology 2018 Vol.27 Issue 3, pp.105-108

Document Type:

Article

Publication Date:

7/2018

Document Language:

English

Document ID (Org No.):

135879

Frequency:

Quarterly

pISSN:

11102179

DOI:

10.12816/0053607

Descriptors:

Keywords:

Abstract:

 Background: Pseudomonas aeruginosa has developed resistance to multiple antibiotics. Unfortunately, ciprofloxacin-resistant P.aeruginosa has emerged with multiple resistance mechanisms. Objectives: This study aims to determine the frequency of gyrA (Topoisomerase II) mutation and its contribution to ciprofloxacin resistance in P. aeruginosa isolates from patients at Zagazig University Hospitals. Methodology: Cultivation of pus specimens, identification of isolates by Gram stain, oxidase test and API20NE. Antibiotic susceptibility testing and ciprofloxacin minimal inhibitory concentration (MIC) were done for all isolates. PCR –RFLP was used to detect mutation in gyrA gene. Results: Among 192 examined specimens, 30 (15.6%) P. aerguinosa were isolated mainly from Surgery and Burn Unit. Fourteen isolates (46.7%) were resistant to ciprofloxacin. Ten (71.4%, P<0.001) isolates had shown mutation in gyr A gene by PCR-RFLP. Conclusion: Mutation in gyrA gene is a major mechanism of ciprofloxacin resistance in P. aeruginosa.

Article Details

Ciprofloxacin Resistance Due to gyr A Mutation in Pseudomonas Aeruginosa Isolates at Zagazig University Hospitals

Authors:

Hanaa M. El Maghraby | Rehab A. Rabie

Publisher:

The Egyptian Society of Medical Microbiology

Main Topics:

Medicine

Journal:

The Egyptian Journal of Medical Microbiology 2018 Vol.27 Issue 3, pp.105-108

Document Type:

Article

Publication Date:

7/2018

Document Language:

English

Document ID (Org No.):

135879

Frequency:

Quarterly

pISSN:

11102179

DOI:

10.12816/0053607

Descriptors:

PSEUDOMONAS

MUTATION

ANTIBIOTICS

HOSPITALS

PATIENTS

EGYPT

Topoisomerase II (Gyr A)

Ciprofloxacin

Pseudomonas Aeruginosa

Issues

Article Details

Authors: Hanaa M. El Maghraby | Rehab A. Rabie

Publisher: The Egyptian Society of Medical Microbiology

Main Topics: Medicine

Journal: The Egyptian Journal of Medical Microbiology 2018 Vol.27 Issue 3, pp.105-108

Document Type: Article

Publication Date: 7/2018

Document Language: English

Document ID (Org No.): 135879

Frequency: Quarterly

pISSN: 11102179

DOI: 10.12816/0053607

Descriptors:

Issues

Authors:

Hanaa M. El Maghraby | Rehab A. Rabie

Publisher:

The Egyptian Society of Medical Microbiology

Main Topics:

Medicine

Journal:

The Egyptian Journal of Medical Microbiology 2018 Vol.27 Issue 3, pp.105-108

Document Type:

Article

Publication Date:

7/2018

Document Language:

English

Document ID (Org No.):

135879

Frequency:

Quarterly

pISSN:

11102179

DOI:

10.12816/0053607