Effects of Ozone Therapy and Ozonated Olive Oil on Bacterial Translocation in an Experimental Burn Model

Ayten Başak Kilic; Osman Hakan Kocaman; Feryal Gün Soysal

Article Details

Effects of Ozone Therapy and Ozonated Olive Oil on Bacterial Translocation in an Experimental Burn Model

Authors:

Ayten Başak Kilic | Osman Hakan Kocaman | Feryal Gün Soysal

Publisher:

Istanbul University

Main Topics:

Clinical Sciences (Other)

Journal:

Experimed Vol.15 Issue 2, pp.109-116

Document Type:

Article

Publication Date:

8/2025

Document Language:

English

Document ID (Org No.):

2001358565

pISSN:

2667-5846

Keywords:

Abstract:

 Objective: Ozone therapy may reduce the complications associated with bacterial translocation. This study evaluated the effects of ozone therapy on bacterial translocation and intestinal mucosal damage in a rat model of scald burns. Materials and Methods: Thirty-two Wistar albino rats were divided into four groups. After inducing thirddegree burns, Group A control received no treatment, Group B received rectal ozone, Group C was treated with ozonated olive oil topically, and Group D received pure olive oil. Blood, liver, mesenteric lymph nodes MLN, and intestinal samples were cultured, and histopathological examinations were conducted on the terminal ileum sections. Results: Group A had significantly higher bacterial translocation rates in blood cultures p0.033. Groups A and D showed higher bacterial growth in MLN samples compared to Groups B and C p0.000. Similarly, the growth rate observed in the liver samples of Group D rats was significantly higher than that in the other groups p0.001. Histopathological analysis revealed the most severe mucosal damage in Group A p0.000, while Group C exhibited the least damage and no bacterial growth. Conclusions: Our findings clearly demonstrated that ozone therapy, particularly ozonated olive oil, significantly reduces bacterial translocation and intestinal mucosal damage in burn injuries, suggesting its potential therapeutic role in the management of such injuries.

 Objective: Ozone therapy may reduce the complications associated with bacterial translocation. This study evaluated the effects of ozone therapy on bacterial translocation and intestinal mucosal damage in a rat model of scald burns. Materials and Methods: Thirty-two Wistar albino rats were divided into four groups. After inducing thirddegree burns, Group A control received no treatment, Group B received rectal ozone, Group C was treated with ozonated olive oil topically, and Group D received pure olive oil. Blood, liver, mesenteric lymph nodes MLN, and intestinal samples were cultured, and histopathological examinations were conducted on the terminal ileum sections. Results: Group A had significantly higher bacterial translocation rates in blood cultures p0.033. Groups A and D showed higher bacterial growth in MLN samples compared to Groups B and C p0.000. Similarly, the growth rate observed in the liver samples of Group D rats was significantly higher than that in the other groups p0.001. Histopathological analysis revealed the most severe mucosal damage in Group A p0.000, while Group C exhibited the least damage and no bacterial growth. Conclusions: Our findings clearly demonstrated that ozone therapy, particularly ozonated olive oil, significantly reduces bacterial translocation and intestinal mucosal damage in burn injuries, suggesting its potential therapeutic role in the management of such injuries.

 Objective: Ozone therapy may reduce the complications associated with bacterial translocation. This study evaluated the effects of ozone therapy on bacterial translocation and intestinal mucosal damage in a rat model of scald burns. Materials and Methods: Thirty-two Wistar albino rats were divided into four groups. After inducing thirddegree burns, Group A control received no treatment, Group B received rectal ozone, Group C was treated with ozonated olive oil topically, and Group D received pure olive oil. Blood, liver, mesenteric lymph nodes MLN, and intestinal samples were cultured, and histopathological examinations were conducted on the terminal ileum sections. Results: Group A had significantly higher bacterial translocation rates in blood cultures p0.033. Groups A and D showed higher bacterial growth in MLN samples compared to Groups B and C p0.000. Similarly, the growth rate observed in the liver samples of Group D rats was significantly higher than that in the other groups p0.001. Histopathological analysis revealed the most severe mucosal damage in Group A p0.000, while Group C exhibited the least damage and no bacterial growth. Conclusions: Our findings clearly demonstrated that ozone therapy, particularly ozonated olive oil, significantly reduces bacterial translocation and intestinal mucosal damage in burn injuries, suggesting its potential therapeutic role in the management of such injuries.

 Objective: Ozone therapy may reduce the complications associated with bacterial translocation. This study evaluated the effects of ozone therapy on bacterial translocation and intestinal mucosal damage in a rat model of scald burns. Materials and Methods: Thirty-two Wistar albino rats were divided into four groups. After inducing thirddegree burns, Group A control received no treatment, Group B received rectal ozone, Group C was treated with ozonated olive oil topically, and Group D received pure olive oil. Blood, liver, mesenteric lymph nodes MLN, and intestinal samples were cultured, and histopathological examinations were conducted on the terminal ileum sections. Results: Group A had significantly higher bacterial translocation rates in blood cultures p0.033. Groups A and D showed higher bacterial growth in MLN samples compared to Groups B and C p0.000. Similarly, the growth rate observed in the liver samples of Group D rats was significantly higher than that in the other groups p0.001. Histopathological analysis revealed the most severe mucosal damage in Group A p0.000, while Group C exhibited the least damage and no bacterial growth. Conclusions: Our findings clearly demonstrated that ozone therapy, particularly ozonated olive oil, significantly reduces bacterial translocation and intestinal mucosal damage in burn injuries, suggesting its potential therapeutic role in the management of such injuries.

Article Details

Effects of Ozone Therapy and Ozonated Olive Oil on Bacterial Translocation in an Experimental Burn Model

Authors:

Ayten Başak Kilic | Osman Hakan Kocaman | Feryal Gün Soysal

Publisher:

Istanbul University

Main Topics:

Clinical Sciences (Other)

Journal:

Experimed Vol.15 Issue 2, pp.109-116

Document Type:

Article

Publication Date:

8/2025

Document Language:

English

Document ID (Org No.):

2001358565

pISSN:

2667-5846

Burn

Ozone (O3)

Ozone therapy

Bacterial translocation

Rat

Issues

Article Details

Effects of Ozone Therapy and Ozonated Olive Oil on Bacterial Translocation in an Experimental Burn Model

Authors: Ayten Başak Kilic | Osman Hakan Kocaman | Feryal Gün Soysal

Publisher: Istanbul University

Main Topics: Clinical Sciences (Other)

Journal: Experimed Vol.15 Issue 2, pp.109-116

Document Type: Article

Publication Date: 8/2025

Document Language: English

Document ID (Org No.): 2001358565

pISSN: 2667-5846

Burn

Ozone (O3)

Ozone therapy

Bacterial translocation

Rat

Issues

Authors:

Ayten Başak Kilic | Osman Hakan Kocaman | Feryal Gün Soysal

Publisher:

Istanbul University

Main Topics:

Clinical Sciences (Other)

Journal:

Experimed Vol.15 Issue 2, pp.109-116

Document Type:

Article

Publication Date:

8/2025

Document Language:

English

Document ID (Org No.):

2001358565

pISSN:

2667-5846