First report of GTG-banded nomenclature of Pakistani Lohi sheep (Ovis aries)

Ahmad ALI; Masroor Ellahi BABAR; Saqib MAHMOOD; Muhammad IMRAN

Article Details

First report of GTG-banded nomenclature of Pakistani Lohi sheep (Ovis aries)

Authors:

Ahmad ALI | Masroor Ellahi BABAR | Saqib MAHMOOD | Muhammad IMRAN

Publisher:

TUBITAK

Journal:

Turkish Journal of Veterinary and Animal Sciences 2011 Vol.35 Issue 4, pp.213-217

Document Type:

Article

Publication Date:

3/2011

Document Language:

Turkish

Document ID (Org No.):

2000767155

Frequency:

Bimonthly

eISSN:

1303-6181

pISSN:

1300-0128

Keywords:

Abstract:

 

The present study was planned to generate the G-band nomenclature of the commonly occurring Lohi sheep breed in the Punjab province of Pakistan. Whole blood samples were collected through jugular venepuncture in heparinised vacutainers.  The metaphase slides were prepared using standard protocols. The results from microscopic screening of 50 good quality metaphase cells from each animal were studied and they revealed a chromosome complement of 54, XX and XY in Lohi sheep. The first 3 pairs were sub/metacentric whereas the other autosomes, including X, were classed as acro/subacrocentric. The Y chromosome was the smallest metacentric chromosome and had a star-like presentation. The trypsinisation time for optimal G-bands was highly variable, and individual chromosomes were identified by G-positive and negative band markers. The identification of chromosomes 4 & 6, 8 & 9, and 19-26 was difficult because these chromosomes were indistinct and small. The X chromosome was the largest sub/acrocentric, with a prominent G-positive band along the lower middle length. Complete G-band homology was noticed between Lohi and other reported sheep breeds from different parts of the world. The G-band ideogram of Lohi was also constructed based upon the G-band organisation observed in the Lohi genome. None of the 350 metaphase cells studied revealed chromosomal abnormalities.

 

The present study was planned to generate the G-band nomenclature of the commonly occurring Lohi sheep breed in the Punjab province of Pakistan. Whole blood samples were collected through jugular venepuncture in heparinised vacutainers.  The metaphase slides were prepared using standard protocols. The results from microscopic screening of 50 good quality metaphase cells from each animal were studied and they revealed a chromosome complement of 54, XX and XY in Lohi sheep. The first 3 pairs were sub/metacentric whereas the other autosomes, including X, were classed as acro/subacrocentric. The Y chromosome was the smallest metacentric chromosome and had a star-like presentation. The trypsinisation time for optimal G-bands was highly variable, and individual chromosomes were identified by G-positive and negative band markers. The identification of chromosomes 4 & 6, 8 & 9, and 19-26 was difficult because these chromosomes were indistinct and small. The X chromosome was the largest sub/acrocentric, with a prominent G-positive band along the lower middle length. Complete G-band homology was noticed between Lohi and other reported sheep breeds from different parts of the world. The G-band ideogram of Lohi was also constructed based upon the G-band organisation observed in the Lohi genome. None of the 350 metaphase cells studied revealed chromosomal abnormalities.

Article Details

First report of GTG-banded nomenclature of Pakistani Lohi sheep (Ovis aries)

Authors:

Ahmad ALI | Masroor Ellahi BABAR | Saqib MAHMOOD | Muhammad IMRAN

Publisher:

TUBITAK

Journal:

Turkish Journal of Veterinary and Animal Sciences 2011 Vol.35 Issue 4, pp.213-217

Document Type:

Article

Publication Date:

3/2011

Document Language:

Turkish

Document ID (Org No.):

2000767155

Frequency:

Bimonthly

eISSN:

1303-6181

pISSN:

1300-0128

Key words: Lohi sheep

karyotype

GTG bands

Pakistan

cytogenetics

Issues

Article Details

First report of GTG-banded nomenclature of Pakistani Lohi sheep (Ovis aries)

Authors: Ahmad ALI | Masroor Ellahi BABAR | Saqib MAHMOOD | Muhammad IMRAN

Publisher: TUBITAK

Journal: Turkish Journal of Veterinary and Animal Sciences 2011 Vol.35 Issue 4, pp.213-217

Document Type: Article

Publication Date: 3/2011

Document Language: Turkish

Document ID (Org No.): 2000767155

Frequency: Bimonthly

eISSN: 1303-6181

pISSN: 1300-0128

Key words: Lohi sheep

karyotype

GTG bands

Pakistan

cytogenetics

Issues

Authors:

Ahmad ALI | Masroor Ellahi BABAR | Saqib MAHMOOD | Muhammad IMRAN

Publisher:

TUBITAK

Journal:

Turkish Journal of Veterinary and Animal Sciences 2011 Vol.35 Issue 4, pp.213-217

Document Type:

Article

Publication Date:

3/2011

Document Language:

Turkish

Document ID (Org No.):

2000767155

Frequency:

Bimonthly

eISSN:

1303-6181

pISSN:

1300-0128