Phytochemical Analysis and Antioxidant, Anticholinesterase and Antiatherogenic Activity of Hairy Tea (Stachys lavandulifolia)

Abdussamat Güzel

Article Details

Phytochemical Analysis and Antioxidant, Anticholinesterase and Antiatherogenic Activity of Hairy Tea (Stachys lavandulifolia)

Authors:

Abdussamat Güzel

Publisher:

Igdir University

Main Topics:

Biyokimya ve Hücre Biyolojisi (Diğer)

Journal:

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.13 Issue 4, pp.2809-2817

Document Type:

Article

Publication Date:

1/2023

Document Language:

Turkish

Document ID (Org No.):

2001302952

pISSN:

2536-4618

Keywords:

Abstract:

 Stachys lavandulifolia Vahl. is a flowering plant family belonging to the Lamiaceae family, which has a great place in ethnobotany. In this study, the phytochemical composition, inhibition of acetylcholinesterase AChE and paraoxonase hPON 1 for antiatherosclerotic activity, and antioxidant capacity of the plant were investigated. Phytochemical content was determined by LC-MSMS system, enzyme inhibition, and antioxidant capacity studies were determined by spectrophotometer. The antioxidant capacity of S. lavandulifolia extracts methanol, hexane, and water was determined by applying ABTS, DPPH, FRAP, and CUPRAC methods. The methanol extract of S. lavandulifolia exhibited significant inhibition of AChE IC50 value for methanol extract 0.1050.17 mgmL R2:0.978. In contrast, methanol and water extracts of S. lavandulifolia showed no inhibitory effect on hPON 1. The highest activity for ABTS was 23.42 in methanol extract and 50.07 for DPPH activity in methanol extract. In the metal-reducing power test, the absorbance was 0.2330.47 for FRAP water extract and 0.5871.52 for CUPRAC methanol extract. According to LC-MSMS analysis, luteolin, fumaric acid, caffeic acid, syringic acid, hydroxybenzoic acid, quercetin, salicylic acid, gallic acid, catechin hydrate, and acetohydroxamic acid were determined in the methanol extract of the plant. In conclusion, S. lavandulifolia, which has antioxidant, anti-atherogenic, and anti-neurodegenerative properties, has the potential to be used as a natural medicine instead of synthetic drugs used in Alzheimers patients.

 Stachys lavandulifolia Vahl. is a flowering plant family belonging to the Lamiaceae family, which has a great place in ethnobotany. In this study, the phytochemical composition, inhibition of acetylcholinesterase AChE and paraoxonase hPON 1 for antiatherosclerotic activity, and antioxidant capacity of the plant were investigated. Phytochemical content was determined by LC-MSMS system, enzyme inhibition, and antioxidant capacity studies were determined by spectrophotometer. The antioxidant capacity of S. lavandulifolia extracts methanol, hexane, and water was determined by applying ABTS, DPPH, FRAP, and CUPRAC methods. The methanol extract of S. lavandulifolia exhibited significant inhibition of AChE IC50 value for methanol extract 0.1050.17 mgmL R2:0.978. In contrast, methanol and water extracts of S. lavandulifolia showed no inhibitory effect on hPON 1. The highest activity for ABTS was 23.42 in methanol extract and 50.07 for DPPH activity in methanol extract. In the metal-reducing power test, the absorbance was 0.2330.47 for FRAP water extract and 0.5871.52 for CUPRAC methanol extract. According to LC-MSMS analysis, luteolin, fumaric acid, caffeic acid, syringic acid, hydroxybenzoic acid, quercetin, salicylic acid, gallic acid, catechin hydrate, and acetohydroxamic acid were determined in the methanol extract of the plant. In conclusion, S. lavandulifolia, which has antioxidant, anti-atherogenic, and anti-neurodegenerative properties, has the potential to be used as a natural medicine instead of synthetic drugs used in Alzheimers patients.

 Stachys lavandulifolia Vahl. is a flowering plant family belonging to the Lamiaceae family, which has a great place in ethnobotany. In this study, the phytochemical composition, inhibition of acetylcholinesterase AChE and paraoxonase hPON 1 for antiatherosclerotic activity, and antioxidant capacity of the plant were investigated. Phytochemical content was determined by LC-MSMS system, enzyme inhibition, and antioxidant capacity studies were determined by spectrophotometer. The antioxidant capacity of S. lavandulifolia extracts methanol, hexane, and water was determined by applying ABTS, DPPH, FRAP, and CUPRAC methods. The methanol extract of S. lavandulifolia exhibited significant inhibition of AChE IC50 value for methanol extract 0.1050.17 mgmL R2:0.978. In contrast, methanol and water extracts of S. lavandulifolia showed no inhibitory effect on hPON 1. The highest activity for ABTS was 23.42 in methanol extract and 50.07 for DPPH activity in methanol extract. In the metal-reducing power test, the absorbance was 0.2330.47 for FRAP water extract and 0.5871.52 for CUPRAC methanol extract. According to LC-MSMS analysis, luteolin, fumaric acid, caffeic acid, syringic acid, hydroxybenzoic acid, quercetin, salicylic acid, gallic acid, catechin hydrate, and acetohydroxamic acid were determined in the methanol extract of the plant. In conclusion, S. lavandulifolia, which has antioxidant, anti-atherogenic, and anti-neurodegenerative properties, has the potential to be used as a natural medicine instead of synthetic drugs used in Alzheimers patients.

 Stachys lavandulifolia Vahl. etnobotanikte önemli yeri olan Lamiaceae familyasına ait çiçekli bitki ailesindendir. Bu çalışmada bitkinin fitokimyasal kompozisyonu, asetilkolinesteraz AChE inhibisyonu, antiaterosklerotik aktivite için paraoksonaz hPON 1 inhibisyonu ve antioksidan kapasitesi araştırıldı. Fitokimyasal içerik LC-MSMS sistemi ile enzim inhibisyonu ve antioksidan kapasite çalışmaları ise spektrofotometre ile belirlendi. S. lavandulifolia ekstraktlarının metanol, hekzan ve su antioksidan kapasitesi ABTS, DPPH, FRAP ve CUPRAC yöntemleri uygulanarak belirlendi. S. lavandulifolia nın metanol ekstraktı AChE üzerinde önemli inhibisyon sergiledi metanol ekstraktı için IC50 değeri 0.105 0.17 mgmL R2:0.978. Buna karşılık, S. lavandulifolianın metanol ve su ekstreleri hPON 1 üzerinde inhibisyon etkisi göstermedi. ABTS için en yüksek aktivite metanol ekstresinde 23.42 ve DPPH aktivitesi için metanol ekstresinde 50.07 olarak belirlendi. Metal indirgeme gücü deneyinde, FRAP su ekstraktı için 0.233 0.47 ve CUPRAC metanol ekstraktı için 0.5871.52 absorbans olarak tespit edildi. Bitkinin metanol ekstraktında LC-MSMS analizlerine göre luteolin, fumarik asit, kafeik asit, siringik asit, hidroksibenzoik asit, kuersetin, salisilik asit, gallik asit, kateşin hidrat ve asetohidroksamik asit saptanmıştır. Sonuç olarak, antioksidan, anti-aterojenik ve anti-nörodejeneratif özelliklere sahip olan S. lavandulifolia, Alzheimer hastalarında kullanılan sentetik ilaçlar yerine doğal bir ilaç olarak kullanılma potansiyeline sahiptir.

Article Details

Phytochemical Analysis and Antioxidant, Anticholinesterase and Antiatherogenic Activity of Hairy Tea (Stachys lavandulifolia)

Authors:

Abdussamat Güzel

Publisher:

Igdir University

Main Topics:

Biyokimya ve Hücre Biyolojisi (Diğer)

Journal:

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.13 Issue 4, pp.2809-2817

Document Type:

Article

Publication Date:

1/2023

Document Language:

Turkish

Document ID (Org No.):

2001302952

pISSN:

2536-4618

Antioxidant

Acetylcholinesterase

Paraoxonase

Stachys lavandulifolia

LC-MS/MSAntioksidan

Asetilkolinesteraz

Paraoksonaz

LC-MS/MS

Stachys lavandulifolia

Issues

Article Details

Authors: Abdussamat Güzel

Publisher: Igdir University

Main Topics: Biyokimya ve Hücre Biyolojisi (Diğer)

Journal: Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.13 Issue 4, pp.2809-2817

Document Type: Article

Publication Date: 1/2023

Document Language: Turkish

Document ID (Org No.): 2001302952

pISSN: 2536-4618

Issues

Authors:

Abdussamat Güzel

Publisher:

Igdir University

Main Topics:

Biyokimya ve Hücre Biyolojisi (Diğer)

Journal:

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.13 Issue 4, pp.2809-2817

Document Type:

Article

Publication Date:

1/2023

Document Language:

Turkish

Document ID (Org No.):

2001302952

pISSN:

2536-4618