Characterization of Escherichia coli Isolates from Commercial Layer Hens with Salpingitis

Mahmood Salehi; Reza Ghanbarpour

doi:10.3844/ajavsp.2010.208.214

Article Details

Characterization of Escherichia coli Isolates from Commercial Layer Hens with Salpingitis

Authors:

Mahmood Salehi | Reza Ghanbarpour

Publisher:

Science Publications

Journal:

American Journal of Animal and Veterinary Sciences Vol.5 Issue 3, pp.208-214

Document Type:

Article

Publication Date:

9/2010

Document Language:

English

Document ID (Org No.):

2001396282

pISSN:

1557-4563

DOI:

10.3844/ajavsp.2010.208.214

Keywords:

Abstract:

 Problem statement: Escherichia coli isolates are the most common bacterial agents of
salpingitis in commercial layer hens. The objectives of this study were to determine the phylogenetic
groups/subgroups and antibiotic resistance of E. coli isolates from salpingitis cases in commercial layer
hens farms in southeast of Iran. Approach: One hundred twenty one E. coli isolates from oviducts of
layer hens with salpingitis were examined to determine their O-serogroup, phylogenetic
group/subgroup and antibiotic resistance pattern. Results: O-serogroup determination test showed that
47 (38.84%) isolates were typeable and belonged to eleven different O serogroups including: O1, O2,
O6, O8, O15, O20, O25, O36, O78, O86 and O111 and 71 (58.67%) isolates were O-nontypeable.
Three most prevalent serogroup were O78, O2 and O1 respectively. PCR assays showed that the
isolates fall into four phylogenetic groups A (41.32%), D (33.88%), B2 (14.87%) and B1 (9.91%).
Ninety nine (81.81%) isolates fell into six phylogenetic subgroups including: A0 (17.35%), A1
(23.96%), B22 (5.78%), B23 (9.09%), D1 (13.22%) and D2 (20.66%). All of the E. coli isolates from
O78 serogroup belonged to A phylo-group whereas the O2 isolates mostly fell into D group. The
maximum antibiotic resistance was against tetracycline (100%) and minimum resistance was against
linco-spectin (37.19%). Twenty four isolates (19.83%) were resistant to all of the examined antibiotics.
Twenty three different patterns of multiple drug resistance were observed, out of which N-Te-Sxt-Fm-
Nfx-C and N-Te-Sxt-Gm-Fm-Nfx-C-Ls was the two most frequent patterns respectively. The resistant
isolates were found in all of the phylogenetic groups and or sub groups. Conclusion: There are
similarities between salpingitis derived E. coli strains and other avian pathogenic E. coli isolates in
phylogroups, O-serogroups and antibio-resistance patterns.

Article Details

Characterization of Escherichia coli Isolates from Commercial Layer Hens with Salpingitis

Authors:

Mahmood Salehi | Reza Ghanbarpour

Publisher:

Science Publications

Journal:

American Journal of Animal and Veterinary Sciences Vol.5 Issue 3, pp.208-214

Document Type:

Article

Publication Date:

9/2010

Document Language:

English

Document ID (Org No.):

2001396282

pISSN:

1557-4563

DOI:

10.3844/ajavsp.2010.208.214

Escherichia coli

antibiotic

phylotyping

salpingitis

Issues

Article Details

Characterization of Escherichia coli Isolates from Commercial Layer Hens with Salpingitis

Authors: Mahmood Salehi | Reza Ghanbarpour

Publisher: Science Publications

Journal: American Journal of Animal and Veterinary Sciences Vol.5 Issue 3, pp.208-214

Document Type: Article

Publication Date: 9/2010

Document Language: English

Document ID (Org No.): 2001396282

pISSN: 1557-4563

DOI: 10.3844/ajavsp.2010.208.214

Escherichia coli

antibiotic

phylotyping

salpingitis

Issues

Authors:

Mahmood Salehi | Reza Ghanbarpour

Publisher:

Science Publications

Journal:

American Journal of Animal and Veterinary Sciences Vol.5 Issue 3, pp.208-214

Document Type:

Article

Publication Date:

9/2010

Document Language:

English

Document ID (Org No.):

2001396282

pISSN:

1557-4563

DOI:

10.3844/ajavsp.2010.208.214