Method Validation and Calculation of Measurement Uncertainty for Salt Determination in Cheese by Mohr Method

Yasin Yakar; Elif Esra Altuner; Tuba Tekeli; Yener Tekeli; Merve Özdemir; Tuğçe Çelik

Article Details

Method Validation and Calculation of Measurement Uncertainty for Salt Determination in Cheese by Mohr Method

Authors:

Yasin Yakar | Elif Esra Altuner | Tuba Tekeli | Yener Tekeli | Merve Özdemir | Tuğçe Çelik

Publisher:

Ordu University

Main Topics:

Analitik Kimya (Diğer)

Journal:

Ordu Üniversitesi Bilim ve Teknoloji Dergisi Vol.15 Issue 1, pp.142-150

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001352226

pISSN:

2146-6440

Keywords:

Abstract:

 Validation studies were conducted to verify the validity of the Mohr method, which is one of the most common methods for salt determination in the laboratory. Precision repeatability and reproducibility and accuracy are method validation parameters. Relative standard deviation RSD values obtained under reproducibility and repeatability conditions were compared with concentration-dependent precision values, and the results were appropriate. The accuracy study was carried out using a standard reference substance. The t-test was used to determine whether the results were close to the true value, and the results were found to be appropriate. As a result, the validation studies of the Mohr method were completed and are valid for our laboratory. A study on the measurement uncertainty of cheese salt was also conducted. Therefore, Urfa cheese and kashar cheese were preferred. The applied analysis method determined the uncertainty components known as weight, volume, precision, and accuracy. Then, the expanded uncertainty was calculated by combining the uncertainties resulting from these uncertainties. The expanded uncertainty is Usalt 0.028.

 Validation studies were conducted to verify the validity of the Mohr method, which is one of the most common methods for salt determination in the laboratory. Precision repeatability and reproducibility and accuracy are method validation parameters. Relative standard deviation RSD values obtained under reproducibility and repeatability conditions were compared with concentration-dependent precision values, and the results were appropriate. The accuracy study was carried out using a standard reference substance. The t-test was used to determine whether the results were close to the true value, and the results were found to be appropriate. As a result, the validation studies of the Mohr method were completed and are valid for our laboratory. A study on the measurement uncertainty of cheese salt was also conducted. Therefore, Urfa cheese and kashar cheese were preferred. The applied analysis method determined the uncertainty components known as weight, volume, precision, and accuracy. Then, the expanded uncertainty was calculated by combining the uncertainties resulting from these uncertainties. The expanded uncertainty is Usalt 0.028.

 Validation studies were conducted to verify the validity of the Mohr method, which is one of the most common methods for salt determination in the laboratory. Precision repeatability and reproducibility and accuracy are method validation parameters. Relative standard deviation RSD values obtained under reproducibility and repeatability conditions were compared with concentration-dependent precision values, and the results were appropriate. The accuracy study was carried out using a standard reference substance. The t-test was used to determine whether the results were close to the true value, and the results were found to be appropriate. As a result, the validation studies of the Mohr method were completed and are valid for our laboratory. A study on the measurement uncertainty of cheese salt was also conducted. Therefore, Urfa cheese and kashar cheese were preferred. The applied analysis method determined the uncertainty components known as weight, volume, precision, and accuracy. Then, the expanded uncertainty was calculated by combining the uncertainties resulting from these uncertainties. The expanded uncertainty is Usalt 0.028.

 Laboratuvarda tuz tayini için en yaygın kullanılan yöntemlerden biri olan Mohr yönteminin geçerliliğini doğrulamak için validasyon çalışmaları yapılmıştır. Kesinlik tekrarlanabilirlik ve tekrar üretilebilirlik ve doğruluk yöntem validasyon parametreleridir. Tekrar üretilebilirlik ve tekrar üretilebilirlik koşulları altında elde edilen Bağıl Standart Sapma RSD değerleri konsantrasyona bağlı kesinlik değerleri ile karşılaştırılmış ve sonuçlar uygun bulunmuştur. Doğruluk çalışması standart referans madde kullanılarak yapılmıştır. Sonuçların gerçek değere yakın olup olmadığını belirlemek için t-testi kullanılmış ve sonuçlar uygun bulunmuştur. Sonuç olarak Mohr yönteminin validasyon çalışmaları tamamlanmış ve laboratuvarımız için geçerlidir. Peynir tuzunun ölçüm belirsizliği üzerine bir çalışma da yapılmıştır. Bu nedenle Urfa peyniri ve kaşar peyniri tercih edilmiştir. Uygulanan analiz yöntemi ile ağırlık, hacim, kesinlik ve doğruluk olarak bilinen belirsizlik bileşenleri belirlenmiştir. Daha sonra bu belirsizliklerden kaynaklanan belirsizlikler birleştirilerek genişletilmiş belirsizlik hesaplanmıştır. Genişletilmiş belirsizlik Utuz0,028dir.

Article Details

Method Validation and Calculation of Measurement Uncertainty for Salt Determination in Cheese by Mohr Method

Authors:

Yasin Yakar | Elif Esra Altuner | Tuba Tekeli | Yener Tekeli | Merve Özdemir | Tuğçe Çelik

Publisher:

Ordu University

Main Topics:

Analitik Kimya (Diğer)

Journal:

Ordu Üniversitesi Bilim ve Teknoloji Dergisi Vol.15 Issue 1, pp.142-150

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001352226

pISSN:

2146-6440

cheese

salt

validation

measurement

Mohr methodpeynir

tuz

doğrulama

ölçüm

Mohr metodu

Issues

Article Details

Authors: Yasin Yakar | Elif Esra Altuner | Tuba Tekeli | Yener Tekeli | Merve Özdemir | Tuğçe Çelik

Publisher: Ordu University

Main Topics: Analitik Kimya (Diğer)

Journal: Ordu Üniversitesi Bilim ve Teknoloji Dergisi Vol.15 Issue 1, pp.142-150

Document Type: Article

Publication Date: 6/2025

Document Language: English

Document ID (Org No.): 2001352226

pISSN: 2146-6440

Issues

Authors:

Yasin Yakar | Elif Esra Altuner | Tuba Tekeli | Yener Tekeli | Merve Özdemir | Tuğçe Çelik

Publisher:

Ordu University

Main Topics:

Analitik Kimya (Diğer)

Journal:

Ordu Üniversitesi Bilim ve Teknoloji Dergisi Vol.15 Issue 1, pp.142-150

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001352226

pISSN:

2146-6440