INVESTIGATION OF THE EFFECTS OF PREGABALIN ON WOUND HEALING IN L929 FIBROBLAST CELLS

Hüseyin Bekmez; Hamza Halıcı

Article Details

INVESTIGATION OF THE EFFECTS OF PREGABALIN ON WOUND HEALING IN L929 FIBROBLAST CELLS

Authors:

Hüseyin Bekmez | Hamza Halıcı

Publisher:

Ataturk University

Main Topics:

Tıbbi Farmakoloji

Journal:

Current Research in Health Sciences Vol.1 Issue 1, pp.8-14

Document Type:

Article

Publication Date:

2/2024

Document Language:

English

Document ID (Org No.):

2001312569

pISSN:

3023-6991

Keywords:

Abstract:

 Aim: Wound healing is a multifaceted, complex process consisting of sequential and interrelated phases including hemostasisinflammation phase, proliferation phase and remodeling phase. Pregabalin PGB, a gabapentin derivative, is an anticonvulsant agent with anti-inflammatory and antioxidant properties. Therefore, in this study, we aimed to show the effect of pregabalin on cell viability in L929 fibroblast cells and its effects on fibroblast migration and wound closure during the wound healing process. Materials And Methods: In this study, the effect of different concentrations of pregabalin on cell viability and proliferation in L929 skin fibroblast cells was investigated using MTT assay. In addition, a scratch wound healing model was established in L929 skin fibroblast cells and the effects of pregabalin concentrations that increase cell proliferation on wound healing in MTT assay were shown. At the end of the experiment, TGF-β1 levels of all groups were measured by ELISA method. Results: In our studies, it was observed that 100, 50, 25, 10 µM concentrations of pregabalin increased cell proliferation. In the scratch wound healing model, pregabalin at concentrations of 100 and 50 µM showed a significant closure compared to control and other groups. TGF-β1 levels were decreased in groups with good healing scores 50, 25 µM. Conclusion: Pregabalin has been shown to enhance wound healing in in vitro experiments. This effect needs to be evaluated holistically within the organ system in vivo. There is also a need for experimental and clinical studies to evaluate the wound healing effects and mechanism of pregabalin.

 Aim: Wound healing is a multifaceted, complex process consisting of sequential and interrelated phases including hemostasisinflammation phase, proliferation phase and remodeling phase. Pregabalin PGB, a gabapentin derivative, is an anticonvulsant agent with anti-inflammatory and antioxidant properties. Therefore, in this study, we aimed to show the effect of pregabalin on cell viability in L929 fibroblast cells and its effects on fibroblast migration and wound closure during the wound healing process. Materials And Methods: In this study, the effect of different concentrations of pregabalin on cell viability and proliferation in L929 skin fibroblast cells was investigated using MTT assay. In addition, a scratch wound healing model was established in L929 skin fibroblast cells and the effects of pregabalin concentrations that increase cell proliferation on wound healing in MTT assay were shown. At the end of the experiment, TGF-β1 levels of all groups were measured by ELISA method. Results: In our studies, it was observed that 100, 50, 25, 10 µM concentrations of pregabalin increased cell proliferation. In the scratch wound healing model, pregabalin at concentrations of 100 and 50 µM showed a significant closure compared to control and other groups. TGF-β1 levels were decreased in groups with good healing scores 50, 25 µM. Conclusion: Pregabalin has been shown to enhance wound healing in in vitro experiments. This effect needs to be evaluated holistically within the organ system in vivo. There is also a need for experimental and clinical studies to evaluate the wound healing effects and mechanism of pregabalin.

 Aim: Wound healing is a multifaceted, complex process consisting of sequential and interrelated phases including hemostasisinflammation phase, proliferation phase and remodeling phase. Pregabalin PGB, a gabapentin derivative, is an anticonvulsant agent with anti-inflammatory and antioxidant properties. Therefore, in this study, we aimed to show the effect of pregabalin on cell viability in L929 fibroblast cells and its effects on fibroblast migration and wound closure during the wound healing process. Materials And Methods: In this study, the effect of different concentrations of pregabalin on cell viability and proliferation in L929 skin fibroblast cells was investigated using MTT assay. In addition, a scratch wound healing model was established in L929 skin fibroblast cells and the effects of pregabalin concentrations that increase cell proliferation on wound healing in MTT assay were shown. At the end of the experiment, TGF-β1 levels of all groups were measured by ELISA method. Results: In our studies, it was observed that 100, 50, 25, 10 µM concentrations of pregabalin increased cell proliferation. In the scratch wound healing model, pregabalin at concentrations of 100 and 50 µM showed a significant closure compared to control and other groups. TGF-β1 levels were decreased in groups with good healing scores 50, 25 µM. Conclusion: Pregabalin has been shown to enhance wound healing in in vitro experiments. This effect needs to be evaluated holistically within the organ system in vivo. There is also a need for experimental and clinical studies to evaluate the wound healing effects and mechanism of pregabalin.

 Aim: Wound healing is a multifaceted, complex process consisting of sequential and interrelated phases including hemostasisinflammation phase, proliferation phase and remodeling phase. Pregabalin PGB, a gabapentin derivative, is an anticonvulsant agent with anti-inflammatory and antioxidant properties. Therefore, in this study, we aimed to show the effect of pregabalin on cell viability in L929 fibroblast cells and its effects on fibroblast migration and wound closure during the wound healing process. Materials And Methods: In this study, the effect of different concentrations of pregabalin on cell viability and proliferation in L929 skin fibroblast cells was investigated using MTT assay. In addition, a scratch wound healing model was established in L929 skin fibroblast cells and the effects of pregabalin concentrations that increase cell proliferation on wound healing in MTT assay were shown. At the end of the experiment, TGF-β1 levels of all groups were measured by ELISA method. Results: In our studies, it was observed that 100, 50, 25, 10 µM concentrations of pregabalin increased cell proliferation. In the scratch wound healing model, pregabalin at concentrations of 100 and 50 µM showed a significant closure compared to control and other groups. TGF-β1 levels were decreased in groups with good healing scores 50, 25 µM. Conclusion: Pregabalin has been shown to enhance wound healing in in vitro experiments. This effect needs to be evaluated holistically within the organ system in vivo. There is also a need for experimental and clinical studies to evaluate the wound healing effects and mechanism of pregabalin.

Article Details

INVESTIGATION OF THE EFFECTS OF PREGABALIN ON WOUND HEALING IN L929 FIBROBLAST CELLS

Authors:

Hüseyin Bekmez | Hamza Halıcı

Publisher:

Ataturk University

Main Topics:

Tıbbi Farmakoloji

Journal:

Current Research in Health Sciences Vol.1 Issue 1, pp.8-14

Document Type:

Article

Publication Date:

2/2024

Document Language:

English

Document ID (Org No.):

2001312569

pISSN:

3023-6991

Wound Healing

pregabalin

scratch wound assay

L929 cell line

Issues

Article Details

INVESTIGATION OF THE EFFECTS OF PREGABALIN ON WOUND HEALING IN L929 FIBROBLAST CELLS

Authors: Hüseyin Bekmez | Hamza Halıcı

Publisher: Ataturk University

Main Topics: Tıbbi Farmakoloji

Journal: Current Research in Health Sciences Vol.1 Issue 1, pp.8-14

Document Type: Article

Publication Date: 2/2024

Document Language: English

Document ID (Org No.): 2001312569

pISSN: 3023-6991

Wound Healing

pregabalin

scratch wound assay

L929 cell line

Issues

Authors:

Hüseyin Bekmez | Hamza Halıcı

Publisher:

Ataturk University

Main Topics:

Tıbbi Farmakoloji

Journal:

Current Research in Health Sciences Vol.1 Issue 1, pp.8-14

Document Type:

Article

Publication Date:

2/2024

Document Language:

English

Document ID (Org No.):

2001312569

pISSN:

3023-6991