A Comparative Study of Detection of Bordetella avium Antibodies in Turkeys by ELISA, SPAT, and AGID Test

Süheyla TÜRKYILMAZ; Kenan TÜRKYILMAZ; Osman KAYA

Article Details

A Comparative Study of Detection of Bordetella avium Antibodies in Turkeys by ELISA, SPAT, and AGID Test

Authors:

Süheyla TÜRKYILMAZ | Kenan TÜRKYILMAZ | Osman KAYA

Publisher:

TUBITAK

Main Topics:

Animal husbandry

Journal:

Turkish Journal of Veterinary and Animal Sciences 2006 Vol.30 Issue 2, pp.165-169

Document Type:

Article

Publication Date:

1/2006

Document Language:

English

Document ID (Org No.):

2000768458

Frequency:

Bimonthly

eISSN:

1303-6181

pISSN:

1300-0128

Keywords:

Abstract:

 

The aims of this study were to develop a serum plate agglutination test (SPAT) antigen and agar gel immunodiffusion (AGID) test antigen for the serological detection of turkeys that have been exposed to Bordetella avium; to compare the sensitivity of commercial enzyme-linked immunosorbent assay (ELISA) with SPAT, and AGID test, and to survey B. avium antibodies in turkey flocks in Aydın, Turkey. For these purposes, serum samples collected from 300 turkeys were examined by ELISA, SPAT, and AGID test. The seroprevalence was 44%, 28%, and 15% for ELISA, SPAT, and AGID test, respectively. This study revealed that ELISA was the most sensitive test for the detection of B. avium antibodies; however, it was determined that SPAT and AGID test can also be used in screening for B. avium antibodies.

 

Bu çal›flman›n amaçlar› Bordetella avium’a maruz kalm›fl hindilerin serolojik olarak saptanmas› için serum pleyt aglutinasyon test (SPAT) ve agar jel immunodiffuzyon (AGID) test antijenlerinin gelifltirilmesi, ticari enzimle iflaretli immun deneyi (ELISA) ile SPAT ve AGID testinin duyarl›l›klar›n›n karfl›laflt›r›lmas›, Ayd›n’da hindi sürülerinde B. avium antikorlar›n›n serolojik olarak taranmas›d›r. Bu amaçla, 300 hindiden toplanan serum örnekleri ELISA, SPAT ve AGID test yöntemleriyle incelendi. Seroprevalens ELISA, SPAT ve AGID testi için s›ras›yla % 44, % 28, % 15 olarak belirlendi. Çal›flmada, B. avium antikorlar›n›n tespit edilmesinde en duyarl› testin ELISA oldu¤u; bununla birlikte, SPAT ve AGID testinin de B. avium antikorlar›n›n belirlenmesinde kullan›labilece¤i tespit edildi. .

Article Details

A Comparative Study of Detection of Bordetella avium Antibodies in Turkeys by ELISA, SPAT, and AGID Test

Authors: Süheyla TÜRKYILMAZ | Kenan TÜRKYILMAZ | Osman KAYA

Publisher: TUBITAK

Main Topics: Animal husbandry

Journal: Turkish Journal of Veterinary and Animal Sciences 2006 Vol.30 Issue 2, pp.165-169

Document Type: Article

Publication Date: 1/2006

Document Language: English

Document ID (Org No.): 2000768458

Frequency: Bimonthly

eISSN: 1303-6181

pISSN: 1300-0128

Bordetella avium

ELISA

SPAT

AGID

Issues

Authors:

Süheyla TÜRKYILMAZ | Kenan TÜRKYILMAZ | Osman KAYA

Publisher:

TUBITAK

Main Topics:

Animal husbandry

Journal:

Turkish Journal of Veterinary and Animal Sciences 2006 Vol.30 Issue 2, pp.165-169

Document Type:

Article

Publication Date:

1/2006

Document Language:

English

Document ID (Org No.):

2000768458

Frequency:

Bimonthly

eISSN:

1303-6181

pISSN:

1300-0128