Antifungal Activity of Endophytic Bacillus spp. Bacteria and Its Effect on Root and Coleoptile Length during Germination Period

İnci Güler Güney

Article Details

Antifungal Activity of Endophytic Bacillus spp. Bacteria and Its Effect on Root and Coleoptile Length during Germination Period

Authors:

İnci Güler Güney

Publisher:

Van Yuzuncu Yıl University

Main Topics:

Phytopathology

Journal:

Yuzuncu Yıl University Journal of Agricultural Sciences Vol.35 Issue 2, pp.334-349

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001350714

pISSN:

1308-7576

Keywords:

Abstract:

 In this study, endophytic bacteria were isolated from roots and crowns of rosemary Rosmarinus officinalis L., olive Olea europaea L., and loquat Eriobotrya japonica L. plant samples. The morphological and physiological properties of nine isolated endophytic bacteria were determined. All isolates were identified as Gram-positive, oxidase-positive and catalase-positive. Amylase, cellulase, and carbohydrate tests gave positive results. Antagonistic activities of the isolates against fungal pathogens varied between 85.7 and 52.9 against Fusarium culmorum and between 86.0 and 65.1 against Neoscytalidium dimidiatum. BMBA2 isolate gave the best results both in Petri dish antagonistic activity and in wheat seed germination in terms of root length and coleoptile length. BMBA2 isolates gave the best results with a coleoptile length of 7.58 cm and root length of 8.33 cm. In wheat seeds treated with F. culmorum and bacteria, the BMBA2 isolate gave the best result with a coleoptile length of 6.98 and a root length of 7.30 cm. For the identification of bacteria, in vitro BiBA1 and ND3BA were determined as Bacillus amyloliquefaciens subsp. plantarum; BiBA2 and YDBA as Bacillus subtilis; NDBA, ND2BA, BMBA1, BMBA2, and BMBA3 as Bacillus mojavensis. Since this is the first study to use endophytic Bacillus mojavensis as a biological agent against F. culmorum and N. dimidiatum pathogens, the results obtained from this study are thought to be important and promising in terms of application.

 In this study, endophytic bacteria were isolated from roots and crowns of rosemary Rosmarinus officinalis L., olive Olea europaea L., and loquat Eriobotrya japonica L. plant samples. The morphological and physiological properties of nine isolated endophytic bacteria were determined. All isolates were identified as Gram-positive, oxidase-positive and catalase-positive. Amylase, cellulase, and carbohydrate tests gave positive results. Antagonistic activities of the isolates against fungal pathogens varied between 85.7 and 52.9 against Fusarium culmorum and between 86.0 and 65.1 against Neoscytalidium dimidiatum. BMBA2 isolate gave the best results both in Petri dish antagonistic activity and in wheat seed germination in terms of root length and coleoptile length. BMBA2 isolates gave the best results with a coleoptile length of 7.58 cm and root length of 8.33 cm. In wheat seeds treated with F. culmorum and bacteria, the BMBA2 isolate gave the best result with a coleoptile length of 6.98 and a root length of 7.30 cm. For the identification of bacteria, in vitro BiBA1 and ND3BA were determined as Bacillus amyloliquefaciens subsp. plantarum; BiBA2 and YDBA as Bacillus subtilis; NDBA, ND2BA, BMBA1, BMBA2, and BMBA3 as Bacillus mojavensis. Since this is the first study to use endophytic Bacillus mojavensis as a biological agent against F. culmorum and N. dimidiatum pathogens, the results obtained from this study are thought to be important and promising in terms of application.

 In this study, endophytic bacteria were isolated from roots and crowns of rosemary Rosmarinus officinalis L., olive Olea europaea L., and loquat Eriobotrya japonica L. plant samples. The morphological and physiological properties of nine isolated endophytic bacteria were determined. All isolates were identified as Gram-positive, oxidase-positive and catalase-positive. Amylase, cellulase, and carbohydrate tests gave positive results. Antagonistic activities of the isolates against fungal pathogens varied between 85.7 and 52.9 against Fusarium culmorum and between 86.0 and 65.1 against Neoscytalidium dimidiatum. BMBA2 isolate gave the best results both in Petri dish antagonistic activity and in wheat seed germination in terms of root length and coleoptile length. BMBA2 isolates gave the best results with a coleoptile length of 7.58 cm and root length of 8.33 cm. In wheat seeds treated with F. culmorum and bacteria, the BMBA2 isolate gave the best result with a coleoptile length of 6.98 and a root length of 7.30 cm. For the identification of bacteria, in vitro BiBA1 and ND3BA were determined as Bacillus amyloliquefaciens subsp. plantarum; BiBA2 and YDBA as Bacillus subtilis; NDBA, ND2BA, BMBA1, BMBA2, and BMBA3 as Bacillus mojavensis. Since this is the first study to use endophytic Bacillus mojavensis as a biological agent against F. culmorum and N. dimidiatum pathogens, the results obtained from this study are thought to be important and promising in terms of application.

 In this study, endophytic bacteria were isolated from roots and crowns of rosemary Rosmarinus officinalis L., olive Olea europaea L., and loquat Eriobotrya japonica L. plant samples. The morphological and physiological properties of nine isolated endophytic bacteria were determined. All isolates were identified as Gram-positive, oxidase-positive and catalase-positive. Amylase, cellulase, and carbohydrate tests gave positive results. Antagonistic activities of the isolates against fungal pathogens varied between 85.7 and 52.9 against Fusarium culmorum and between 86.0 and 65.1 against Neoscytalidium dimidiatum. BMBA2 isolate gave the best results both in Petri dish antagonistic activity and in wheat seed germination in terms of root length and coleoptile length. BMBA2 isolates gave the best results with a coleoptile length of 7.58 cm and root length of 8.33 cm. In wheat seeds treated with F. culmorum and bacteria, the BMBA2 isolate gave the best result with a coleoptile length of 6.98 and a root length of 7.30 cm. For the identification of bacteria, in vitro BiBA1 and ND3BA were determined as Bacillus amyloliquefaciens subsp. plantarum; BiBA2 and YDBA as Bacillus subtilis; NDBA, ND2BA, BMBA1, BMBA2, and BMBA3 as Bacillus mojavensis. Since this is the first study to use endophytic Bacillus mojavensis as a biological agent against F. culmorum and N. dimidiatum pathogens, the results obtained from this study are thought to be important and promising in terms of application.

Article Details

Antifungal Activity of Endophytic Bacillus spp. Bacteria and Its Effect on Root and Coleoptile Length during Germination Period

Authors:

İnci Güler Güney

Publisher:

Van Yuzuncu Yıl University

Main Topics:

Phytopathology

Journal:

Yuzuncu Yıl University Journal of Agricultural Sciences Vol.35 Issue 2, pp.334-349

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001350714

pISSN:

1308-7576

N. dimidiatum

antagonist

biological control

Issues

Article Details

Antifungal Activity of Endophytic Bacillus spp. Bacteria and Its Effect on Root and Coleoptile Length during Germination Period

Authors: İnci Güler Güney

Publisher: Van Yuzuncu Yıl University

Main Topics: Phytopathology

Journal: Yuzuncu Yıl University Journal of Agricultural Sciences Vol.35 Issue 2, pp.334-349

Document Type: Article

Publication Date: 6/2025

Document Language: English

Document ID (Org No.): 2001350714

pISSN: 1308-7576

N. dimidiatum

antagonist

biological control

Issues

Authors:

İnci Güler Güney

Publisher:

Van Yuzuncu Yıl University

Main Topics:

Phytopathology

Journal:

Yuzuncu Yıl University Journal of Agricultural Sciences Vol.35 Issue 2, pp.334-349

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001350714

pISSN:

1308-7576