Molecular characterization and genetic diversity analysis of citrus cultivars by RAPD markers

M.n.r. BAIG; Sapna GREWAL; Santosh DHILLON

Article Details

Molecular characterization and genetic diversity analysis of citrus cultivars by RAPD markers

Authors:

M.n.r. BAIG | Sapna GREWAL | Santosh DHILLON

Publisher:

TUBITAK

Journal:

Turkish Journal of Agriculture and Forestry 2009 Vol.33 Issue 4, pp.375-384

Document Type:

Article

Publication Date:

3/2009

Document Language:

Turkish

Document ID (Org No.):

2000808442

Frequency:

Bimonthly

eISSN:

1303-6173

pISSN:

1300-011X

Keywords:

Abstract:

 

Random Amplified Polymorphic DNA (RAPD) markers were used to evaluate genetic similarity and inter-relationship among 18 citrus cultivars, including 13 species and 5 hybrids. Out of 40 decamer primers screened, 25 were selected which produced 250 markers; of which 231 were polymorphic and some species or cultivar specific RAPD markers. The Jaccard coefficient was used to calculate the genetic similarity. UPGMA was used to generate the dendrogram which clearly separated Jatti-Khatti from all major clusters at a similarity coefficient of 0.61. The average genetic similarity value observed across all the genotypes was 0.63, with the 2 sweet orange cultivars, Jaffa and Blood red, showing maximum similarity (82%). The Jatti-Khatti and King Mandarin were found to be genetically most diverse. The genetic variation between cultivars was quite high and revealed their different origins.

 

Random Amplified Polymorphic DNA (RAPD) markers were used to evaluate genetic similarity and inter-relationship among 18 citrus cultivars, including 13 species and 5 hybrids. Out of 40 decamer primers screened, 25 were selected which produced 250 markers; of which 231 were polymorphic and some species or cultivar specific RAPD markers. The Jaccard coefficient was used to calculate the genetic similarity. UPGMA was used to generate the dendrogram which clearly separated Jatti-Khatti from all major clusters at a similarity coefficient of 0.61. The average genetic similarity value observed across all the genotypes was 0.63, with the 2 sweet orange cultivars, Jaffa and Blood red, showing maximum similarity (82%). The Jatti-Khatti and King Mandarin were found to be genetically most diverse. The genetic variation between cultivars was quite high and revealed their different origins.

Article Details

Molecular characterization and genetic diversity analysis of citrus cultivars by RAPD markers

Authors:

M.n.r. BAIG | Sapna GREWAL | Santosh DHILLON

Publisher:

TUBITAK

Journal:

Turkish Journal of Agriculture and Forestry 2009 Vol.33 Issue 4, pp.375-384

Document Type:

Article

Publication Date:

3/2009

Document Language:

Turkish

Document ID (Org No.):

2000808442

Frequency:

Bimonthly

eISSN:

1303-6173

pISSN:

1300-011X

Key words: Citrus

RAPD

UPGMA

Dendrogram

Genetic diversity

PCR

Issues

Article Details

Molecular characterization and genetic diversity analysis of citrus cultivars by RAPD markers

Authors: M.n.r. BAIG | Sapna GREWAL | Santosh DHILLON

Publisher: TUBITAK

Journal: Turkish Journal of Agriculture and Forestry 2009 Vol.33 Issue 4, pp.375-384

Document Type: Article

Publication Date: 3/2009

Document Language: Turkish

Document ID (Org No.): 2000808442

Frequency: Bimonthly

eISSN: 1303-6173

pISSN: 1300-011X

Key words: Citrus

RAPD

UPGMA

Dendrogram

Genetic diversity

PCR

Issues

Authors:

M.n.r. BAIG | Sapna GREWAL | Santosh DHILLON

Publisher:

TUBITAK

Journal:

Turkish Journal of Agriculture and Forestry 2009 Vol.33 Issue 4, pp.375-384

Document Type:

Article

Publication Date:

3/2009

Document Language:

Turkish

Document ID (Org No.):

2000808442

Frequency:

Bimonthly

eISSN:

1303-6173

pISSN:

1300-011X