Production and Characterization of bacterial cellulose from Komagataeibacter xylinus S4 strain

Nazime Dogan; Naime Nur Bozbeyoğlu Kart; Duygu Takanoğlu Bulut; Orhan Karabulut; Erdal Uğuzdoğan

Article Details

Production and Characterization of bacterial cellulose from Komagataeibacter xylinus S4 strain

Authors:

Nazime Dogan | Naime Nur Bozbeyoğlu Kart | Duygu Takanoğlu Bulut | Orhan Karabulut | Erdal Uğuzdoğan

Publisher:

Pamukkale University

Main Topics:

Kimya Mühendisliği (Diğer)

Journal:

Pamukkale Üniversitesi Mühendislik Bilimleri Dergisi Vol.30 Issue 2, pp.271-281

Document Type:

Article

Publication Date:

4/2024

Document Language:

English

Document ID (Org No.):

2001316325

pISSN:

2147-5881

Keywords:

Abstract:

 In this study, bacterial cellulose BC was obtained from Komagataeibacter xylinus S4 and characterized in detail. The effects of a various of carbon sources and media, different pH conditions, incubation temperatures, Surface areaVolume ratios, and incubation durations were determined for BC production. Considering the carbon types, the amount of BC production from high to low was realized as sucrose, fructose, mannitol, xylose, arabinose, and lactose. The highest BC amount 1.303 gL was achieved by combining M1A05P5 broth, 30 C, 1.06 cm-1 Surface areaVolume ratio, pH 3.5 and 21 days. According to scanning electron microscope SEM analysis, the cellulose fibril diameters were 34.87-45.97 nm at pH 3.5 and 29.71-102.3 nm at pH 6.5 in M1A05P5. Also, TGA analysis exhibited that the weight loss of BC in the removal of water step initialized between 50 C and 150 C and the degradation step initialized between 215 C and 228 C. Finally, the electrical conductivity values of the BC samples were determined on the 27-137 C temperature scale. It was observed that the conductivity was temperature dependent, and the conductivity increased exponentially as the temperature increased. In conclusion, the cellulose from K. xylinus S4 typically showed a semiconducting behavior.

 In this study, bacterial cellulose BC was obtained from Komagataeibacter xylinus S4 and characterized in detail. The effects of a various of carbon sources and media, different pH conditions, incubation temperatures, Surface areaVolume ratios, and incubation durations were determined for BC production. Considering the carbon types, the amount of BC production from high to low was realized as sucrose, fructose, mannitol, xylose, arabinose, and lactose. The highest BC amount 1.303 gL was achieved by combining M1A05P5 broth, 30 C, 1.06 cm-1 Surface areaVolume ratio, pH 3.5 and 21 days. According to scanning electron microscope SEM analysis, the cellulose fibril diameters were 34.87-45.97 nm at pH 3.5 and 29.71-102.3 nm at pH 6.5 in M1A05P5. Also, TGA analysis exhibited that the weight loss of BC in the removal of water step initialized between 50 C and 150 C and the degradation step initialized between 215 C and 228 C. Finally, the electrical conductivity values of the BC samples were determined on the 27-137 C temperature scale. It was observed that the conductivity was temperature dependent, and the conductivity increased exponentially as the temperature increased. In conclusion, the cellulose from K. xylinus S4 typically showed a semiconducting behavior.

 In this study, bacterial cellulose BC was obtained from Komagataeibacter xylinus S4 and characterized in detail. The effects of a various of carbon sources and media, different pH conditions, incubation temperatures, Surface areaVolume ratios, and incubation durations were determined for BC production. Considering the carbon types, the amount of BC production from high to low was realized as sucrose, fructose, mannitol, xylose, arabinose, and lactose. The highest BC amount 1.303 gL was achieved by combining M1A05P5 broth, 30 C, 1.06 cm-1 Surface areaVolume ratio, pH 3.5 and 21 days. According to scanning electron microscope SEM analysis, the cellulose fibril diameters were 34.87-45.97 nm at pH 3.5 and 29.71-102.3 nm at pH 6.5 in M1A05P5. Also, TGA analysis exhibited that the weight loss of BC in the removal of water step initialized between 50 C and 150 C and the degradation step initialized between 215 C and 228 C. Finally, the electrical conductivity values of the BC samples were determined on the 27-137 C temperature scale. It was observed that the conductivity was temperature dependent, and the conductivity increased exponentially as the temperature increased. In conclusion, the cellulose from K. xylinus S4 typically showed a semiconducting behavior.

 Bu çalışmada, Komagataeibacter xylinus S4ten elde edilen bakteriyel selüloz BS detaylı şekilde karakterize edilmiştir. Çeşitli karbon kaynakları ve ortamlarının, farklı pH şartları, sıcaklıklar, yüzey alanıhacim oranları ve inkübasyon zamanlarının BS üretimine etkisi tespit edilmiştir. Karbon tipleri gözönüne alındığında, yüksekten düşüğe doğru BS üretim miktarı, sükroz, fruktoz, mannitol, ksiloz, arabinoz ve laktoz şeklinde gerçekleşmiştir. En yüksek BS miktarına 1.303 gL, M1A05P5 sıvı besiyeri, 30 C sıcaklık, 1.06 cm-1 yüzey alanıhacim oranı, pH 3.5 ve 21 gün kombinasyonunda ulaşılmıştır. Taramalı elektron mikroskobu SEM analizine göre M1A05P5 ortamında üretilen bakteriyel selüloz liflerinin çapları pH 3.5te 34.87-45.97 nm değerindeyken pH 6.5 değerine yükseldiğinde lif çapları 29.71-102.3 nm olarak ölçülmüştür. Ayrıca, TGA analizi, BS numunelerinde dehidrasyon adımındaki ağırlık kaybının 50 C ile 150 C arasında, bozunma adımının ise 215 C ile 228 C arasında başladığını göstermiştir. Son olarak, BS örneklerinin elektriksel iletkenlik değerleri 27-137 C sıcaklık skalasında tespit edildi. İletkenliğin sıcaklığa bağlı olduğu ve sıcaklık arttıkça iletkenliğin üstel olarak arttığı gözlendi. Sonuç olarak, K. xylinus S4 selülozu tipik olarak yarı iletken bir davranış göstermiştir.

Article Details

Production and Characterization of bacterial cellulose from Komagataeibacter xylinus S4 strain

Authors:

Nazime Dogan | Naime Nur Bozbeyoğlu Kart | Duygu Takanoğlu Bulut | Orhan Karabulut | Erdal Uğuzdoğan

Publisher:

Pamukkale University

Main Topics:

Kimya Mühendisliği (Diğer)

Journal:

Pamukkale Üniversitesi Mühendislik Bilimleri Dergisi Vol.30 Issue 2, pp.271-281

Document Type:

Article

Publication Date:

4/2024

Document Language:

English

Document ID (Org No.):

2001316325

pISSN:

2147-5881

Bacterial cellulose

Local isolate

Carbon sources

Electrical conductivity

Thermal analysis

SEMBakteriyel selüloz

Yerel izolat

Karbon kaynaklari

Elektriksel iletkenlik

Termal analiz

SEM

Issues

Article Details

Authors: Nazime Dogan | Naime Nur Bozbeyoğlu Kart | Duygu Takanoğlu Bulut | Orhan Karabulut | Erdal Uğuzdoğan

Publisher: Pamukkale University

Main Topics: Kimya Mühendisliği (Diğer)

Journal: Pamukkale Üniversitesi Mühendislik Bilimleri Dergisi Vol.30 Issue 2, pp.271-281

Document Type: Article

Publication Date: 4/2024

Document Language: English

Document ID (Org No.): 2001316325

pISSN: 2147-5881

Issues

Authors:

Nazime Dogan | Naime Nur Bozbeyoğlu Kart | Duygu Takanoğlu Bulut | Orhan Karabulut | Erdal Uğuzdoğan

Publisher:

Pamukkale University

Main Topics:

Kimya Mühendisliği (Diğer)

Journal:

Pamukkale Üniversitesi Mühendislik Bilimleri Dergisi Vol.30 Issue 2, pp.271-281

Document Type:

Article

Publication Date:

4/2024

Document Language:

English

Document ID (Org No.):

2001316325

pISSN:

2147-5881