Uterus-Relaxing Study of a Sudanese Herb (El-Hazha)

Aimun Abdelgaffar Elhassan Ahmed; Robert Gaspar; Arpad Marki; Andrea Vasas; Mahmoud Mudawi Eltahir Mudawi; Judit Hohmann; George Falkay

doi:10.3844/ajbbsp.2010.231.238

Article Details

Uterus-Relaxing Study of a Sudanese Herb (El-Hazha)

Authors:

Aimun Abdelgaffar Elhassan Ahmed | Robert Gaspar | Arpad Marki | Andrea Vasas | Mahmoud Mudawi Eltahir Mudawi | Judit Hohmann | George Falkay

Publisher:

Science Publications

Journal:

American Journal of Biochemistry and Biotechnology Vol.6 Issue 3, pp.231-238

Document Type:

Article

Publication Date:

9/2010

Document Language:

English

Document ID (Org No.):

2001397058

pISSN:

1558-6332

DOI:

10.3844/ajbbsp.2010.231.238

Keywords:

Abstract:

 Problem statement: The aim of this study is to investigate the pharmacological effects of the Methanolic-extract (AH2) of El-Hazha and its sub-fractions. Approach: These investigations were carried out on in vitro isolated uterus preparations from Non-Pregnant (NP) and Late-Pregnant rats (LP). In parallel displacement radio-ligand binding assay was performed for β-Adrenergic Receptors (β-ADR). Results: Showed that the herb and its different fractions produced dose-dependent relaxant effect (p<0.05, t-test, n = 6) on uterine contraction elicit by 25 mM KCl in both NP and LP uteri that affected significantly by fractionation, however, the effect of the most active fraction (AH2-11) was reversed by prior addition of propranolol (non-specific β-antagonist), but not affected by progesterone pre treatment of the LP rats. In addition, AH2 only in high concentration displaced isotopes from β-ADR, this affinity changed markedly by fractionation. Conclusion: We validate the fractionation effect on its relaxant activity and found partial role for β-ADR on mediating this activity. Future study was recommended to isolate and investigate its active components to enhance this activity or to discover a new novel natural therapeutic agent(s).

Article Details

Uterus-Relaxing Study of a Sudanese Herb (El-Hazha)

Authors:

Aimun Abdelgaffar Elhassan Ahmed | Robert Gaspar | Arpad Marki | Andrea Vasas | Mahmoud Mudawi Eltahir Mudawi | Judit Hohmann | George Falkay

Publisher:

Science Publications

Journal:

American Journal of Biochemistry and Biotechnology Vol.6 Issue 3, pp.231-238

Document Type:

Article

Publication Date:

9/2010

Document Language:

English

Document ID (Org No.):

2001397058

pISSN:

1558-6332

DOI:

10.3844/ajbbsp.2010.231.238

Haplophylium tuberculatum

radio-ligand binding assay

&beta

-Adrenergic Receptors (&beta

-ADR)

Non-Pregnant (NP)

Late-Pregnant rats (LP)

Sudanese plant

charles-river laboratories

medicinal and aromatic plants

tocolytic agent

pharmacological effects

progesterone treatment

uterus-relaxing study

Issues

Article Details

Authors: Aimun Abdelgaffar Elhassan Ahmed | Robert Gaspar | Arpad Marki | Andrea Vasas | Mahmoud Mudawi Eltahir Mudawi | Judit Hohmann | George Falkay

Publisher: Science Publications

Journal: American Journal of Biochemistry and Biotechnology Vol.6 Issue 3, pp.231-238

Document Type: Article

Publication Date: 9/2010

Document Language: English

Document ID (Org No.): 2001397058

pISSN: 1558-6332

DOI: 10.3844/ajbbsp.2010.231.238

Issues

Authors:

Aimun Abdelgaffar Elhassan Ahmed | Robert Gaspar | Arpad Marki | Andrea Vasas | Mahmoud Mudawi Eltahir Mudawi | Judit Hohmann | George Falkay

Publisher:

Science Publications

Journal:

American Journal of Biochemistry and Biotechnology Vol.6 Issue 3, pp.231-238

Document Type:

Article

Publication Date:

9/2010

Document Language:

English

Document ID (Org No.):

2001397058

pISSN:

1558-6332

DOI:

10.3844/ajbbsp.2010.231.238