An LC-MS/MS method development for dapagliflozin-loaded nanostructured lipid carrier formulation in rabbit plasma

N.A.

Article Details

An LC-MS/MS method development for dapagliflozin-loaded nanostructured lipid carrier formulation in rabbit plasma

Authors:

N.A.

Publisher:

Marmara University

Main Topics:

Pharmaceutical Analytical Chemistry

Journal:

Journal of Research in Pharmacy Vol.28 Issue 2, pp.438-446

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001346198

pISSN:

2630-6344

Keywords:

Abstract:

 The primary goal of this study was to develop and validate an LC-MSMS method for the detection of dapagliflozin in nanostructured lipid carriers NLC using ion-interaction chromatography. The reversed-phase InfinityLab Poroshell 120 150  4.6 mm, 4 µm column, using a mobile phase of acetonitrile-25 mM ammonium acetate solution with pH 4.1 35:65, vv, effectively separated the analytes and their internal standards. This method has been thoroughly tested and validated to ensure accurate and reliable results. To improve sensitivity and selectivity, mass spectrometry was used in polarity switching mode. In order to study ion transitions for dapagliflozin in both positive and negative mode, multiple reaction monitoring mode was utilized, with the ion transitions being mz 467.1 MCH3COO- 329.1. The assays linear calibration range for dapagliflozin was established from 0.05-150 ngmL to improve drug pharmacokinetics assessment. The analytes limit of detection LOD and limit of quantitation LOQ were 0.07 and 0.35 ngmL, respectively. After testing, no interference was observed in plasma matrices from different sources, including haemolysed and lipemic plasma. The impact of Dapagliflozin-loaded NLC on plasma levels were investigated using this method.

 The primary goal of this study was to develop and validate an LC-MSMS method for the detection of dapagliflozin in nanostructured lipid carriers NLC using ion-interaction chromatography. The reversed-phase InfinityLab Poroshell 120 150  4.6 mm, 4 µm column, using a mobile phase of acetonitrile-25 mM ammonium acetate solution with pH 4.1 35:65, vv, effectively separated the analytes and their internal standards. This method has been thoroughly tested and validated to ensure accurate and reliable results. To improve sensitivity and selectivity, mass spectrometry was used in polarity switching mode. In order to study ion transitions for dapagliflozin in both positive and negative mode, multiple reaction monitoring mode was utilized, with the ion transitions being mz 467.1 MCH3COO- 329.1. The assays linear calibration range for dapagliflozin was established from 0.05-150 ngmL to improve drug pharmacokinetics assessment. The analytes limit of detection LOD and limit of quantitation LOQ were 0.07 and 0.35 ngmL, respectively. After testing, no interference was observed in plasma matrices from different sources, including haemolysed and lipemic plasma. The impact of Dapagliflozin-loaded NLC on plasma levels were investigated using this method.

 The primary goal of this study was to develop and validate an LC-MSMS method for the detection of dapagliflozin in nanostructured lipid carriers NLC using ion-interaction chromatography. The reversed-phase InfinityLab Poroshell 120 150  4.6 mm, 4 µm column, using a mobile phase of acetonitrile-25 mM ammonium acetate solution with pH 4.1 35:65, vv, effectively separated the analytes and their internal standards. This method has been thoroughly tested and validated to ensure accurate and reliable results. To improve sensitivity and selectivity, mass spectrometry was used in polarity switching mode. In order to study ion transitions for dapagliflozin in both positive and negative mode, multiple reaction monitoring mode was utilized, with the ion transitions being mz 467.1 MCH3COO- 329.1. The assays linear calibration range for dapagliflozin was established from 0.05-150 ngmL to improve drug pharmacokinetics assessment. The analytes limit of detection LOD and limit of quantitation LOQ were 0.07 and 0.35 ngmL, respectively. After testing, no interference was observed in plasma matrices from different sources, including haemolysed and lipemic plasma. The impact of Dapagliflozin-loaded NLC on plasma levels were investigated using this method.

 The primary goal of this study was to develop and validate an LC-MSMS method for the detection of dapagliflozin in nanostructured lipid carriers NLC using ion-interaction chromatography. The reversed-phase InfinityLab Poroshell 120 150  4.6 mm, 4 µm column, using a mobile phase of acetonitrile-25 mM ammonium acetate solution with pH 4.1 35:65, vv, effectively separated the analytes and their internal standards. This method has been thoroughly tested and validated to ensure accurate and reliable results. To improve sensitivity and selectivity, mass spectrometry was used in polarity switching mode. In order to study ion transitions for dapagliflozin in both positive and negative mode, multiple reaction monitoring mode was utilized, with the ion transitions being mz 467.1 MCH3COO- 329.1. The assays linear calibration range for dapagliflozin was established from 0.05-150 ngmL to improve drug pharmacokinetics assessment. The analytes limit of detection LOD and limit of quantitation LOQ were 0.07 and 0.35 ngmL, respectively. After testing, no interference was observed in plasma matrices from different sources, including haemolysed and lipemic plasma. The impact of Dapagliflozin-loaded NLC on plasma levels were investigated using this method.

Article Details

An LC-MS/MS method development for dapagliflozin-loaded nanostructured lipid carrier formulation in rabbit plasma

Authors:

N.A.

Publisher:

Marmara University

Main Topics:

Pharmaceutical Analytical Chemistry

Journal:

Journal of Research in Pharmacy Vol.28 Issue 2, pp.438-446

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001346198

pISSN:

2630-6344

Nanostructured lipid carriers

quantitative method

SGLT-2 inhibitors

dapagliflozin

plasma extraction

Issues

Article Details

An LC-MS/MS method development for dapagliflozin-loaded nanostructured lipid carrier formulation in rabbit plasma

Authors: N.A.

Publisher: Marmara University

Main Topics: Pharmaceutical Analytical Chemistry

Journal: Journal of Research in Pharmacy Vol.28 Issue 2, pp.438-446

Document Type: Article

Publication Date: 6/2025

Document Language: English

Document ID (Org No.): 2001346198

pISSN: 2630-6344

Nanostructured lipid carriers

quantitative method

SGLT-2 inhibitors

dapagliflozin

plasma extraction

Issues

Authors:

N.A.

Publisher:

Marmara University

Main Topics:

Pharmaceutical Analytical Chemistry

Journal:

Journal of Research in Pharmacy Vol.28 Issue 2, pp.438-446

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001346198

pISSN:

2630-6344