Oxidative / Nitrosative Stress in Rats Subjected to Focal Cerebral Ischemia / Reperfusion

Hiba A. Awooda; Mohamed F. Lutfi; Gihan M. Sharara G.; Amal M.Saeed

doi:10.12816/0024679

Article Details

Oxidative / Nitrosative Stress in Rats Subjected to Focal Cerebral Ischemia / Reperfusion

Authors:

Hiba A. Awooda | Mohamed F. Lutfi | Gihan M. Sharara G. | Amal M.Saeed

Publisher:

Al-Qassim University

Main Topics:

Medicine

Journal:

International Journal of Health Sciences 2015 Vol 9 Issue 1, pp.17-24

Document Type:

Article

Publication Date:

3/2015

Document Language:

English

Document ID (Org No.):

84651

Frequency:

Tri-Annual

pISSN:

16583639

DOI:

10.12816/0024679

Descriptors:

Keywords:

Abstract:

 Background: Ischemic stroke usually initiates inflammation and oxidative/nitrosative stress leading to neuronal death. Aim: To investigate the existence of oxidative/nitrosativestress in rats subjected to focal cerebral ischemia/reperfusion and its effects on the consequent neurological deficits. Material and Method: Experimental procedures were performed on 30 adult males Wister rats. In the test group, transient focal cerebral ischemia was induced in 15 rats by occlusion of the left common carotid artery (CCA) for 30 minutes followed by reperfusion for 24 hours. Another 15 rats underwent the surgery at the same neck region without occlusion of CCA and served as a control group.Neurobehavioral tests were evaluated, the levels of malondialdehyde (MDA), total antioxidant capacity (TAC) and nitric oxide (NO) metabolites were measured in the serum and brain tissue to detect the effect of surgery on in each group. Result: The serum and brain tissue levels of MDA and NO in the test group were significantly higher compared to the control group (P < 0.001). In contrast, serum and brain tissue levels of TAC of rats subjected to ischemia reperfusion was significantly lower compared to the sham operated rats (P < 0.001). Neurological deficit of the test group correlated positively with serum TAC (CC = 0.937, P = 0.000) and brain tissue TAC (CC = 0.949, P = 0.000) and negatively with serum MDA (CC = -0.949, P = 0.000), brain tissue MDA (CC = -0.963, P = 0.000), serum NO (CC = -0.942, P = 0.000) and brain tissue NO (CC = -0.952, P = 0.000). Conclusion: The study provided further evidence for the presence of oxidative/nitrosative stress in rats subjected to cerebral ischemia/reperfusion and demonstrates a relationship between oxidative/nitrosative biomarkers and the consequent neurological deficits.

Article Details

Oxidative / Nitrosative Stress in Rats Subjected to Focal Cerebral Ischemia / Reperfusion

Authors:

Hiba A. Awooda | Mohamed F. Lutfi | Gihan M. Sharara G. | Amal M.Saeed

Publisher:

Al-Qassim University

Main Topics:

Medicine

Journal:

International Journal of Health Sciences 2015 Vol 9 Issue 1, pp.17-24

Document Type:

Article

Publication Date:

3/2015

Document Language:

English

Document ID (Org No.):

84651

Frequency:

Tri-Annual

pISSN:

16583639

DOI:

10.12816/0024679

Descriptors:

NERVOUS SYSTEM DISEASES

MENTAL STRESS

LABORATORY ANIMALS

DIAGNOSIS

THERAPY

Cerebral

Ischemia/reperfusion

L-NAME

Malondialdehyde

Nitric oxide

Total antioxidant capacity

Issues

Article Details

Authors: Hiba A. Awooda | Mohamed F. Lutfi | Gihan M. Sharara G. | Amal M.Saeed

Publisher: Al-Qassim University

Main Topics: Medicine

Journal: International Journal of Health Sciences 2015 Vol 9 Issue 1, pp.17-24

Document Type: Article

Publication Date: 3/2015

Document Language: English

Document ID (Org No.): 84651

Frequency: Tri-Annual

pISSN: 16583639

DOI: 10.12816/0024679

Descriptors:

Issues

Authors:

Hiba A. Awooda | Mohamed F. Lutfi | Gihan M. Sharara G. | Amal M.Saeed

Publisher:

Al-Qassim University

Main Topics:

Medicine

Journal:

International Journal of Health Sciences 2015 Vol 9 Issue 1, pp.17-24

Document Type:

Article

Publication Date:

3/2015

Document Language:

English

Document ID (Org No.):

84651

Frequency:

Tri-Annual

pISSN:

16583639

DOI:

10.12816/0024679