Thrombophilic Genes Mutations in Women with Repeated In-Vitro Fertilization Failure

Naglaa Fathy Al Husseini; Ahmad Y. Rezk; Mosad M. Odah; Shaymaa M. Abd El Rahman; Amal Idris Ali

Article Details

Thrombophilic Genes Mutations in Women with Repeated In-Vitro Fertilization Failure

Authors:

Naglaa Fathy Al Husseini | Ahmad Y. Rezk | Mosad M. Odah | Shaymaa M. Abd El Rahman | Amal Idris Ali

Publisher:

Science Publications

Journal:

Current Research in Medicine Vol.2 Issue 1, pp.7-12

Document Type:

Article

Publication Date:

3/2011

Document Language:

English

Document ID (Org No.):

2001533722

pISSN:

2524-2202

Keywords:

Abstract:

 Problem statement: Thrombophilia has been recently implicated in early pregnancy loss
and IVF implantation failure, by impairing the initial vascularization process occurring at implantation,
which is necessary for a successful pregnancy. Approach: The aim of this study is to assess the
presence possibility of mutation in thrombophilic genes [Factor V (FV) gene, Prothrombin (PT) gene
and Methylenetetrahydrofolate Reductase (MTHFR) gene] in women with repeated IVF-embryo
transfer failure with unknown causes. The study was performed on patients with two or more
previously failed IVF-embryo transfer cycles with unknown causes. Women who conceived
spontaneously with no previous history of miscarriage and women who have had successful pregnancy
after their first IVF-embryo transfer cycle are included as control groups. Results: There are increase
in allelic frequencies of thrombophilic genes (factor V, prothrombin and methylenetetrahydrofolate
reductase) mutation, in addition to genotype frequencies (both homozygote and heterozygote) and
increase in frequency of multiple gene mutations among women with IVF- embryo transfer failure.
Conclusion: Thrombophilia has a significant role in IVF- embryo transfer implantation failure.
Women with repeated IVF- embryo transfer failure should be screened for Thrombophilia to avoid
repeated IVF- embryo transfer failure.

Article Details

Thrombophilic Genes Mutations in Women with Repeated In-Vitro Fertilization Failure

Authors:

Naglaa Fathy Al Husseini | Ahmad Y. Rezk | Mosad M. Odah | Shaymaa M. Abd El Rahman | Amal Idris Ali

Publisher:

Science Publications

Journal:

Current Research in Medicine Vol.2 Issue 1, pp.7-12

Document Type:

Article

Publication Date:

3/2011

Document Language:

English

Document ID (Org No.):

2001533722

pISSN:

2524-2202

Thrombophilia-mutation-IVF-multiplex PCR-Reverse hybridization

Recurrent Pregnancy Loss (RPL)

Methylenetetrahydrofolate Reductase (MTHFR)

Nitro Blue Tetrazolium (NBT)

In-Vitro Fertilization (IVF)

Intracytoplasmic Sperm Injection (ICSI)

Prothrombin (PT)

Body Mass Index (BMI)

Factor V Ledien (FVL)

Issues

Article Details

Authors: Naglaa Fathy Al Husseini | Ahmad Y. Rezk | Mosad M. Odah | Shaymaa M. Abd El Rahman | Amal Idris Ali

Publisher: Science Publications

Journal: Current Research in Medicine Vol.2 Issue 1, pp.7-12

Document Type: Article

Publication Date: 3/2011

Document Language: English

Document ID (Org No.): 2001533722

pISSN: 2524-2202

Issues

Authors:

Naglaa Fathy Al Husseini | Ahmad Y. Rezk | Mosad M. Odah | Shaymaa M. Abd El Rahman | Amal Idris Ali

Publisher:

Science Publications

Journal:

Current Research in Medicine Vol.2 Issue 1, pp.7-12

Document Type:

Article

Publication Date:

3/2011

Document Language:

English

Document ID (Org No.):

2001533722

pISSN:

2524-2202