Crude Extracts of Three Iris Species as Sources of MRSA Antimicrobial Compounds

Belmina Saric Medic; Anesa Jerković- Mujkić; Berina Cubara; Adaleta Durmic- Pasic; Jasna Hanjalic Kurtovic; Kasim Bajrović; Elma Omeragić; Mirza Dedić; Faruk Bogunić; Lejla Pojskic

Article Details

Crude Extracts of Three Iris Species as Sources of MRSA Antimicrobial Compounds

Authors:

Belmina Saric Medic | Anesa Jerković- Mujkić | Berina Cubara | Adaleta Durmic- Pasic | Jasna Hanjalic Kurtovic | Kasim Bajrović | Elma Omeragić | Mirza Dedić | Faruk Bogunić | Lejla Pojskic

Publisher:

Istanbul University

Main Topics:

Genomics and Transcriptomics | Bacteriology

Journal:

European Journal of Biology Vol.83 Issue 2, pp.182-188

Document Type:

Article

Publication Date:

12/2024

Document Language:

English

Document ID (Org No.):

2001340833

pISSN:

2602-2575

Keywords:

Abstract:

 Objective: Iris species are widely used in pharmaceutical and cosmetic applications owing to their high content of bioactive compounds with anti-inflammatory and antimicrobial properties. This study aimed to investigate the potential antibacterial effect of crude extracts aqueous, 50 and 80 ethanol of three Iris species I. pumila, while I. reichenbachii and I. illyrica are endemic from Bosnia and Herzegovina against the multiresistant bacterial strain methicillin-resistant Staphylococcus aureus subsp. aureus ATCC 33591 MRSA strain. Materials and Methods: The antimicrobial compounds in the crude extracts were identified using High-performance liquid chromatography HPLC, and their effects on the MRSA strain were tested using agar well diffusion and broth microdilution method. The binding affinities were analysed using molecular docking simulations. Results: We identified bioactive targeted compounds in these extracts, mainly flavonoids named isorhamnetin, hesperidin, quercetin, fisetin, genistein, and kaempferol. Antibacterial assays showed that extracts of all three Iris species inhibited MRSA. The binding affinity analysis showed that isorhamnetin and hesperidin had the highest affinity scores, stronger isorhamnetin or the same hesperidin as the positive control ceftobiprole. Conclusion: Thisin vitro and in silico study showed that Iris species represent a valuable source of bioactive compounds that can be used against multidrug-resistant strains such as MRSA. The potential use of these agents in multiple drugs is warranted, and further evaluation for human application is needed.

 Objective: Iris species are widely used in pharmaceutical and cosmetic applications owing to their high content of bioactive compounds with anti-inflammatory and antimicrobial properties. This study aimed to investigate the potential antibacterial effect of crude extracts aqueous, 50 and 80 ethanol of three Iris species I. pumila, while I. reichenbachii and I. illyrica are endemic from Bosnia and Herzegovina against the multiresistant bacterial strain methicillin-resistant Staphylococcus aureus subsp. aureus ATCC 33591 MRSA strain. Materials and Methods: The antimicrobial compounds in the crude extracts were identified using High-performance liquid chromatography HPLC, and their effects on the MRSA strain were tested using agar well diffusion and broth microdilution method. The binding affinities were analysed using molecular docking simulations. Results: We identified bioactive targeted compounds in these extracts, mainly flavonoids named isorhamnetin, hesperidin, quercetin, fisetin, genistein, and kaempferol. Antibacterial assays showed that extracts of all three Iris species inhibited MRSA. The binding affinity analysis showed that isorhamnetin and hesperidin had the highest affinity scores, stronger isorhamnetin or the same hesperidin as the positive control ceftobiprole. Conclusion: Thisin vitro and in silico study showed that Iris species represent a valuable source of bioactive compounds that can be used against multidrug-resistant strains such as MRSA. The potential use of these agents in multiple drugs is warranted, and further evaluation for human application is needed.

 Objective: Iris species are widely used in pharmaceutical and cosmetic applications owing to their high content of bioactive compounds with anti-inflammatory and antimicrobial properties. This study aimed to investigate the potential antibacterial effect of crude extracts aqueous, 50 and 80 ethanol of three Iris species I. pumila, while I. reichenbachii and I. illyrica are endemic from Bosnia and Herzegovina against the multiresistant bacterial strain methicillin-resistant Staphylococcus aureus subsp. aureus ATCC 33591 MRSA strain. Materials and Methods: The antimicrobial compounds in the crude extracts were identified using High-performance liquid chromatography HPLC, and their effects on the MRSA strain were tested using agar well diffusion and broth microdilution method. The binding affinities were analysed using molecular docking simulations. Results: We identified bioactive targeted compounds in these extracts, mainly flavonoids named isorhamnetin, hesperidin, quercetin, fisetin, genistein, and kaempferol. Antibacterial assays showed that extracts of all three Iris species inhibited MRSA. The binding affinity analysis showed that isorhamnetin and hesperidin had the highest affinity scores, stronger isorhamnetin or the same hesperidin as the positive control ceftobiprole. Conclusion: Thisin vitro and in silico study showed that Iris species represent a valuable source of bioactive compounds that can be used against multidrug-resistant strains such as MRSA. The potential use of these agents in multiple drugs is warranted, and further evaluation for human application is needed.

 Objective: Iris species are widely used in pharmaceutical and cosmetic applications owing to their high content of bioactive compounds with anti-inflammatory and antimicrobial properties. This study aimed to investigate the potential antibacterial effect of crude extracts aqueous, 50 and 80 ethanol of three Iris species I. pumila, while I. reichenbachii and I. illyrica are endemic from Bosnia and Herzegovina against the multiresistant bacterial strain methicillin-resistant Staphylococcus aureus subsp. aureus ATCC 33591 MRSA strain. Materials and Methods: The antimicrobial compounds in the crude extracts were identified using High-performance liquid chromatography HPLC, and their effects on the MRSA strain were tested using agar well diffusion and broth microdilution method. The binding affinities were analysed using molecular docking simulations. Results: We identified bioactive targeted compounds in these extracts, mainly flavonoids named isorhamnetin, hesperidin, quercetin, fisetin, genistein, and kaempferol. Antibacterial assays showed that extracts of all three Iris species inhibited MRSA. The binding affinity analysis showed that isorhamnetin and hesperidin had the highest affinity scores, stronger isorhamnetin or the same hesperidin as the positive control ceftobiprole. Conclusion: Thisin vitro and in silico study showed that Iris species represent a valuable source of bioactive compounds that can be used against multidrug-resistant strains such as MRSA. The potential use of these agents in multiple drugs is warranted, and further evaluation for human application is needed.

Article Details

Crude Extracts of Three Iris Species as Sources of MRSA Antimicrobial Compounds

Authors:

Belmina Saric Medic | Anesa Jerković- Mujkić | Berina Cubara | Adaleta Durmic- Pasic | Jasna Hanjalic Kurtovic | Kasim Bajrović | Elma Omeragić | Mirza Dedić | Faruk Bogunić | Lejla Pojskic

Publisher:

Istanbul University

Main Topics:

Genomics and Transcriptomics | Bacteriology

Journal:

European Journal of Biology Vol.83 Issue 2, pp.182-188

Document Type:

Article

Publication Date:

12/2024

Document Language:

English

Document ID (Org No.):

2001340833

pISSN:

2602-2575

Plant bioactive compounds

Methicillin-resistant Staphylococcus aureus

Molecular docking

Minimum inhibitory concentration

Issues

Article Details

Crude Extracts of Three Iris Species as Sources of MRSA Antimicrobial Compounds

Authors: Belmina Saric Medic | Anesa Jerković- Mujkić | Berina Cubara | Adaleta Durmic- Pasic | Jasna Hanjalic Kurtovic | Kasim Bajrović | Elma Omeragić | Mirza Dedić | Faruk Bogunić | Lejla Pojskic

Publisher: Istanbul University

Main Topics: Genomics and Transcriptomics | Bacteriology

Journal: European Journal of Biology Vol.83 Issue 2, pp.182-188

Document Type: Article

Publication Date: 12/2024

Document Language: English

Document ID (Org No.): 2001340833

pISSN: 2602-2575

Plant bioactive compounds

Methicillin-resistant Staphylococcus aureus

Molecular docking

Minimum inhibitory concentration

Issues

Authors:

Belmina Saric Medic | Anesa Jerković- Mujkić | Berina Cubara | Adaleta Durmic- Pasic | Jasna Hanjalic Kurtovic | Kasim Bajrović | Elma Omeragić | Mirza Dedić | Faruk Bogunić | Lejla Pojskic

Publisher:

Istanbul University

Main Topics:

Genomics and Transcriptomics | Bacteriology

Journal:

European Journal of Biology Vol.83 Issue 2, pp.182-188

Document Type:

Article

Publication Date:

12/2024

Document Language:

English

Document ID (Org No.):

2001340833

pISSN:

2602-2575