Protective Effect of Mesenchymal Stem Cells and Melatonin on Testicular Torsion-Induced Infertility

Gül Neşet; Nazlı Çil; Gülçin Abban Mete

Article Details

Protective Effect of Mesenchymal Stem Cells and Melatonin on Testicular Torsion-Induced Infertility

Authors:

Gül Neşet | Nazlı Çil | Gülçin Abban Mete

Publisher:

Bursa Uludağ University

Main Topics:

Biyokimya ve Hücre Biyolojisi (Diğer) | Üreme Tıbbı (Diğer)

Journal:

Uludağ Üniversitesi Tıp Fakültesi Dergisi Vol.50 Issue 2, pp.215-222

Document Type:

Article

Publication Date:

8/2024

Document Language:

English

Document ID (Org No.):

2001329213

pISSN:

1300-414X

Keywords:

Abstract:

 We aimed to explore the effects of systemic melatonin and intratesticular Adipose tissue-derived mesenchymal stem cells AdMSCs administration on rats with acute unilateral testicular torsion. Rats were randomized into Sham group S n8, torsiondetorsion group TD by torsion of right testis with rotated 720 counter clockwise for 3 h, then detorsion n8, Melatonin group given 25 mgkg after torsiondetorsion M n8, Adipose tissue-derived mesenchymal stem cell-treated group after torsiondetorsion MSC n8, Adipose tissue-derived mesenchymal stem cell-treated group with melatonin after torsiondetorsion MSCM n8. We measured MDA, Testosterone, FSH and LH levels, performed histopathological analyses in testicles, and identified SOX, VASA and Caspas-3 reactions immunohistochemically. Testosterone, FSH, LH values did not yield any significant difference between the groups. While the Johnson score in the right testis remained the lowest in TD, the highest score was noted in the S. The TD manifested some degenerative seminiferous tubules, abnormal spermatogenesis and maturation arrest. The degenerative appearance monitored in M, MSC and MSCM groups persisted in some tubules, while markedly reduced degeneration was observed in some other tubules. The highest Caspase-3 expression in TD, whereas SOX-9 expression remained significantly higher in the treatment groups. Another aspect deserving attention is that MSC were characterized by low VASA expression. Our experimental trial suggests that the torsion-induced degeneration in testicular tissue was ameliorated in all the treatment groups. Although MSC, MSCM and M administrations decreased the torsion-induced degeneration in the testicular tissue, these treatments did not prove to be superior to each other.

 We aimed to explore the effects of systemic melatonin and intratesticular Adipose tissue-derived mesenchymal stem cells AdMSCs administration on rats with acute unilateral testicular torsion. Rats were randomized into Sham group S n8, torsiondetorsion group TD by torsion of right testis with rotated 720 counter clockwise for 3 h, then detorsion n8, Melatonin group given 25 mgkg after torsiondetorsion M n8, Adipose tissue-derived mesenchymal stem cell-treated group after torsiondetorsion MSC n8, Adipose tissue-derived mesenchymal stem cell-treated group with melatonin after torsiondetorsion MSCM n8. We measured MDA, Testosterone, FSH and LH levels, performed histopathological analyses in testicles, and identified SOX, VASA and Caspas-3 reactions immunohistochemically. Testosterone, FSH, LH values did not yield any significant difference between the groups. While the Johnson score in the right testis remained the lowest in TD, the highest score was noted in the S. The TD manifested some degenerative seminiferous tubules, abnormal spermatogenesis and maturation arrest. The degenerative appearance monitored in M, MSC and MSCM groups persisted in some tubules, while markedly reduced degeneration was observed in some other tubules. The highest Caspase-3 expression in TD, whereas SOX-9 expression remained significantly higher in the treatment groups. Another aspect deserving attention is that MSC were characterized by low VASA expression. Our experimental trial suggests that the torsion-induced degeneration in testicular tissue was ameliorated in all the treatment groups. Although MSC, MSCM and M administrations decreased the torsion-induced degeneration in the testicular tissue, these treatments did not prove to be superior to each other.

 We aimed to explore the effects of systemic melatonin and intratesticular Adipose tissue-derived mesenchymal stem cells AdMSCs administration on rats with acute unilateral testicular torsion. Rats were randomized into Sham group S n8, torsiondetorsion group TD by torsion of right testis with rotated 720 counter clockwise for 3 h, then detorsion n8, Melatonin group given 25 mgkg after torsiondetorsion M n8, Adipose tissue-derived mesenchymal stem cell-treated group after torsiondetorsion MSC n8, Adipose tissue-derived mesenchymal stem cell-treated group with melatonin after torsiondetorsion MSCM n8. We measured MDA, Testosterone, FSH and LH levels, performed histopathological analyses in testicles, and identified SOX, VASA and Caspas-3 reactions immunohistochemically. Testosterone, FSH, LH values did not yield any significant difference between the groups. While the Johnson score in the right testis remained the lowest in TD, the highest score was noted in the S. The TD manifested some degenerative seminiferous tubules, abnormal spermatogenesis and maturation arrest. The degenerative appearance monitored in M, MSC and MSCM groups persisted in some tubules, while markedly reduced degeneration was observed in some other tubules. The highest Caspase-3 expression in TD, whereas SOX-9 expression remained significantly higher in the treatment groups. Another aspect deserving attention is that MSC were characterized by low VASA expression. Our experimental trial suggests that the torsion-induced degeneration in testicular tissue was ameliorated in all the treatment groups. Although MSC, MSCM and M administrations decreased the torsion-induced degeneration in the testicular tissue, these treatments did not prove to be superior to each other.

 Sistemik melatonin ve intratestiküler adipoz doku kaynaklı mezenkimal kök hücrelerin AdMSCs uygulamasının akut tek taraflı testis torsiyonu olan sıçanlar üzerindeki etkilerini araştırmayı amaçladık. Sıçanlar, Sham grubu S n8, torsiyondetorsiyon grubu sağ testisin 3 saat boyunca saat yönünün tersine 720 döndürülerek torsiyonu ve ardından detorsiyonu ile TD n8, torsiyondetorsiyon sonrası 25 mgkg melatonin verilen grup M n8, torsiyondetorsiyon sonrası adipoz doku kaynaklı mezenkimal kök hücre ile tedavi edilen grup MSC n8, torsiyon detorsiyon sonrası melatonin ile adipoz dokusu kaynaklı mezenkimal kök hücre ile tedavi edilen grup MSCM n8 olarak randomize edildi. MDA, Testosteron, FSH ve LH düzeyleri ölçüldü. Testislerde histopatolojik analizler yapıldı ve SOX, VASA ve Caspas-3 reaksiyonları immünohistokimyasal olarak incelendi. Testosteron, FSH, LH değerleri gruplar arasında anlamlı bir fark göstermedi. Sağ testiste Johnson skoru TDde en düşük seviyede kalırken, en yüksek skor S grubunda kaydedildi. TD grubunda bazı dejeneratif seminifer tübüller, anormal spermatogenez ve olgunlaşma durması görüldü. M, MSC ve MSCM gruplarında izlenen dejeneratif görünüm bazı tübüllerde devam ederken, diğer bazı tübüllerde belirgin şekilde azalmış olduğu gözlendi. En yüksek Kaspaz-3 ekspresyonu TDde görülürken, SOX-9 ekspresyonu tedavi gruplarında anlamlı derecede yüksek kalmıştır. Dikkat edilmesi gereken bir diğer husus da MSCnin düşük VASA ekspresyonu ile karakterize olmasıdır. Deneysel çalışmamız, testis dokusunda torsiyona bağlı dejenerasyonun tüm tedavi gruplarında iyileştiğini göstermektedir. MSC, MSCM ve M uygulamaları testis dokusunda torsiyona bağlı dejenerasyonu azaltmış olsa da, bu tedavilerin birbirlerine üstünlüğü kanıtlanmamıştır.

Article Details

Protective Effect of Mesenchymal Stem Cells and Melatonin on Testicular Torsion-Induced Infertility

Authors:

Gül Neşet | Nazlı Çil | Gülçin Abban Mete

Publisher:

Bursa Uludağ University

Main Topics:

Biyokimya ve Hücre Biyolojisi (Diğer) | Üreme Tıbbı (Diğer)

Journal:

Uludağ Üniversitesi Tıp Fakültesi Dergisi Vol.50 Issue 2, pp.215-222

Document Type:

Article

Publication Date:

8/2024

Document Language:

English

Document ID (Org No.):

2001329213

pISSN:

1300-414X

Testicular Torsion-Detorsion

Stem cell

Melatonin

ApoptosisTestiküler Torsiyon-Detorsiyon

Kök hücre

Melatonin

Apoptozis

Issues

Article Details

Authors: Gül Neşet | Nazlı Çil | Gülçin Abban Mete

Publisher: Bursa Uludağ University

Main Topics: Biyokimya ve Hücre Biyolojisi (Diğer) | Üreme Tıbbı (Diğer)

Journal: Uludağ Üniversitesi Tıp Fakültesi Dergisi Vol.50 Issue 2, pp.215-222

Document Type: Article

Publication Date: 8/2024

Document Language: English

Document ID (Org No.): 2001329213

pISSN: 1300-414X

Issues

Authors:

Gül Neşet | Nazlı Çil | Gülçin Abban Mete

Publisher:

Bursa Uludağ University

Main Topics:

Biyokimya ve Hücre Biyolojisi (Diğer) | Üreme Tıbbı (Diğer)

Journal:

Uludağ Üniversitesi Tıp Fakültesi Dergisi Vol.50 Issue 2, pp.215-222

Document Type:

Article

Publication Date:

8/2024

Document Language:

English

Document ID (Org No.):

2001329213

pISSN:

1300-414X