The Importance of Urinary Enzyme Activities in Dogs With Experimentally  Induced Gentamicin Nephrotoxicity

Mehmet MADEN; Veysi ASLAN

Article Details

The Importance of Urinary Enzyme Activities in Dogs With Experimentally Induced Gentamicin Nephrotoxicity

Authors:

Mehmet MADEN | Veysi ASLAN

Publisher:

TUBITAK

Journal:

Turkish Journal of Veterinary and Animal Sciences 1999 Vol.23 Issue 1, pp.29-42

Document Type:

Article

Publication Date:

12/1998

Document Language:

Turkish

Document ID (Org No.):

2000772037

Frequency:

Bimonthly

eISSN:

1303-6181

pISSN:

1300-0128

Keywords:

Abstract:

 

Totally nine healthy and mature dogs were used in the study. Two of which were served as controls and remaining seven  dogs were assigned to experimental group. Gentamicin sulphate was given to induce nephrotoxicity at the dose of 10 mg/kg body  weight every 8 hours for 10 consecutive days. The samples of blood and urine were taken twice before the experiment and every  other day during gentamicin infection and for 5 days after gentamicin injection was ceased. N-acetyl-?-D-glocosaminidase (NAG), ?-glutamyl transterase (GGT) and Alkaline phosphatase (ALP) and creatinine (CR), total protein (TP), glucose (GLU) and electrolyte (Ca, P, Na, K) concentrations in the urine samples and blood urea nitrogen (BUN), total protein (TP), albumin (ALB), cretainine (SCR), glucose and electrolytes (Ca, P, Na, K) concentrations in the blood samples were measured. Urine dip stick and microscopic examination of urine sediment were performed. Urine protein/creatinine rate and daily protein loss in urine were calculated. The fractionel clearences of electrolytes (Ca, P, Na, K) and glucose were determined. Sodium sulphanilate clearence was measured twice before the experiment and three times during the experiment. Daily clinical examination of all dogs were performed during the experiment. Autopsy and histopatologic examinations were performed in three dogs (1 control, 2 experimental group) on the 11th day of the experiment, two dogs were euthenasied due to uremic coma on the 12th day of the experiment and remaining dogs were necropsied at the end of the experiment.  In this study, acute tubular necrosis induced with nephrotoxic doses of gentamicin was found to be occured between 7th and 10th  days of the experiment on the basis of clinical and laboratory findings. Urine GGT activities were found to be increased (P.

 

Totally nine healthy and mature dogs were used in the study. Two of which were served as controls and remaining seven  dogs were assigned to experimental group. Gentamicin sulphate was given to induce nephrotoxicity at the dose of 10 mg/kg body  weight every 8 hours for 10 consecutive days. The samples of blood and urine were taken twice before the experiment and every  other day during gentamicin infection and for 5 days after gentamicin injection was ceased. N-acetyl-?-D-glocosaminidase (NAG), ?-glutamyl transterase (GGT) and Alkaline phosphatase (ALP) and creatinine (CR), total protein (TP), glucose (GLU) and electrolyte (Ca, P, Na, K) concentrations in the urine samples and blood urea nitrogen (BUN), total protein (TP), albumin (ALB), cretainine (SCR), glucose and electrolytes (Ca, P, Na, K) concentrations in the blood samples were measured. Urine dip stick and microscopic examination of urine sediment were performed. Urine protein/creatinine rate and daily protein loss in urine were calculated. The fractionel clearences of electrolytes (Ca, P, Na, K) and glucose were determined. Sodium sulphanilate clearence was measured twice before the experiment and three times during the experiment. Daily clinical examination of all dogs were performed during the experiment. Autopsy and histopatologic examinations were performed in three dogs (1 control, 2 experimental group) on the 11th day of the experiment, two dogs were euthenasied due to uremic coma on the 12th day of the experiment and remaining dogs were necropsied at the end of the experiment.  In this study, acute tubular necrosis induced with nephrotoxic doses of gentamicin was found to be occured between 7th and 10th  days of the experiment on the basis of clinical and laboratory findings. Urine GGT activities were found to be increased (P.

Article Details

The Importance of Urinary Enzyme Activities in Dogs With Experimentally Induced Gentamicin Nephrotoxicity

Authors:

Mehmet MADEN | Veysi ASLAN

Publisher:

TUBITAK

Journal:

Turkish Journal of Veterinary and Animal Sciences 1999 Vol.23 Issue 1, pp.29-42

Document Type:

Article

Publication Date:

12/1998

Document Language:

Turkish

Document ID (Org No.):

2000772037

Frequency:

Bimonthly

eISSN:

1303-6181

pISSN:

1300-0128

Urinary enzyms

gentamicin nephrotoxicity

dog.

Issues

Article Details

The Importance of Urinary Enzyme Activities in Dogs With Experimentally Induced Gentamicin Nephrotoxicity

Authors: Mehmet MADEN | Veysi ASLAN

Publisher: TUBITAK

Journal: Turkish Journal of Veterinary and Animal Sciences 1999 Vol.23 Issue 1, pp.29-42

Document Type: Article

Publication Date: 12/1998

Document Language: Turkish

Document ID (Org No.): 2000772037

Frequency: Bimonthly

eISSN: 1303-6181

pISSN: 1300-0128

Urinary enzyms

gentamicin nephrotoxicity

dog.

Issues

Authors:

Mehmet MADEN | Veysi ASLAN

Publisher:

TUBITAK

Journal:

Turkish Journal of Veterinary and Animal Sciences 1999 Vol.23 Issue 1, pp.29-42

Document Type:

Article

Publication Date:

12/1998

Document Language:

Turkish

Document ID (Org No.):

2000772037

Frequency:

Bimonthly

eISSN:

1303-6181

pISSN:

1300-0128