THE EFFECT OF ESCHERICHIA COLI BACTERIOPHAGE COCKTAIL ON BACTERIAL CONTAMINATION IN WATER

Hilal Basak Erol

Article Details

THE EFFECT OF ESCHERICHIA COLI BACTERIOPHAGE COCKTAIL ON BACTERIAL CONTAMINATION IN WATER

Authors:

Hilal Basak Erol

Publisher:

Ankara University

Main Topics:

Farmasotik Mikrobiyoloji

Journal:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 1, pp.242-250

Document Type:

Article

Publication Date:

1/2024

Document Language:

English

Document ID (Org No.):

2001300958

pISSN:

1015-3918

Keywords:

Abstract:

 Objective: Wastewater and environmental water resources are treated to eliminate pathogenic microorganisms but contamination is still a significant problem. In particular, the presence of contamination with Escherichia coli E. coli is an important indicator of fecal contamination. Due to increasing antimicrobial resistance and failures of new antimicrobial processes, interest in bacteriophages in pathogen control has increased. Therefore, in our study, phage-based bacteria control in environmental waters was investigated as a natural solution. Material and Method: In our study, E. coli and lytic bacteriophages specific to these E. coli were isolated from environmental water samples in Ankara. The lytic activities of the isolated phages were determined on environmental and clinical extended-spectrum β-lactamases E. coli isolates. Three phages with high lytic activity were selected, and the effectiveness of the single phage and their mixtures on E. coli contamination in water was tested. Result and Discussion: As a result of the study, 17 E. coli strains were isolated from 30 environmental water samples. Lytic bacteriophages in 30 different plaque structures were also isolated from water samples. The isolated phages were found to have lytic activity in the range of 32-70 on the tested bacteria. The effectiveness of three selected phages and their cocktail on E. coli contamination in water was measured at 6th and 24th. As a result, it was observed that the cocktail application reduced the number of host bacteria in the water below detectable limits, also provided a 5-log reduction in non-host test bacteria and maintained its effect for 24 hours. When the results are evaluated, it is thought that cocktail phage application will be an effective method against E. coli contamination in water.

 Objective: Wastewater and environmental water resources are treated to eliminate pathogenic microorganisms but contamination is still a significant problem. In particular, the presence of contamination with Escherichia coli E. coli is an important indicator of fecal contamination. Due to increasing antimicrobial resistance and failures of new antimicrobial processes, interest in bacteriophages in pathogen control has increased. Therefore, in our study, phage-based bacteria control in environmental waters was investigated as a natural solution. Material and Method: In our study, E. coli and lytic bacteriophages specific to these E. coli were isolated from environmental water samples in Ankara. The lytic activities of the isolated phages were determined on environmental and clinical extended-spectrum β-lactamases E. coli isolates. Three phages with high lytic activity were selected, and the effectiveness of the single phage and their mixtures on E. coli contamination in water was tested. Result and Discussion: As a result of the study, 17 E. coli strains were isolated from 30 environmental water samples. Lytic bacteriophages in 30 different plaque structures were also isolated from water samples. The isolated phages were found to have lytic activity in the range of 32-70 on the tested bacteria. The effectiveness of three selected phages and their cocktail on E. coli contamination in water was measured at 6th and 24th. As a result, it was observed that the cocktail application reduced the number of host bacteria in the water below detectable limits, also provided a 5-log reduction in non-host test bacteria and maintained its effect for 24 hours. When the results are evaluated, it is thought that cocktail phage application will be an effective method against E. coli contamination in water.

 Objective: Wastewater and environmental water resources are treated to eliminate pathogenic microorganisms but contamination is still a significant problem. In particular, the presence of contamination with Escherichia coli E. coli is an important indicator of fecal contamination. Due to increasing antimicrobial resistance and failures of new antimicrobial processes, interest in bacteriophages in pathogen control has increased. Therefore, in our study, phage-based bacteria control in environmental waters was investigated as a natural solution. Material and Method: In our study, E. coli and lytic bacteriophages specific to these E. coli were isolated from environmental water samples in Ankara. The lytic activities of the isolated phages were determined on environmental and clinical extended-spectrum β-lactamases E. coli isolates. Three phages with high lytic activity were selected, and the effectiveness of the single phage and their mixtures on E. coli contamination in water was tested. Result and Discussion: As a result of the study, 17 E. coli strains were isolated from 30 environmental water samples. Lytic bacteriophages in 30 different plaque structures were also isolated from water samples. The isolated phages were found to have lytic activity in the range of 32-70 on the tested bacteria. The effectiveness of three selected phages and their cocktail on E. coli contamination in water was measured at 6th and 24th. As a result, it was observed that the cocktail application reduced the number of host bacteria in the water below detectable limits, also provided a 5-log reduction in non-host test bacteria and maintained its effect for 24 hours. When the results are evaluated, it is thought that cocktail phage application will be an effective method against E. coli contamination in water.

 Amaç: Atık su ve çevresel su kaynaklarında patojen mikroorganizmaları ortadan kaldırmak için arıtma yapılsa da bulaş hâlâ önemli bir sorundur. Özellikle Escherichia coli E. coli ile kontaminasyonun varlığı dışkı ile kontaminasyonun önemli bir göstergesidir. Artan antimikrobiyal direnç ve yeni antimikrobiyal geliştirme süreçlerindeki başarısızlıklar nedeniyle patojen kontrolünde bakteriyofajlara olan ilgi artmıştır. Bu nedenle çalışmamızda doğal bir çözüm önerisi olarak çevresel sularda faj bazlı bakteri kontrolü araştırılmıştır. Gereç ve Yöntem: Çalışmamızda Ankara ili çevresel su örneklerinden E. coli ve bu bakterilere özgü litik bakteriyofajlar izole edilmiştir. İzole edilen fajların litik etkinlikleri çevresel ve klinik E. coli izolatları üzerinde belirlenmiştir. Yüksek litik etkinliğe sahip 3 faj seçilerek, fajların tek tek ve karışımlarından hazırlanan kokteylinin sudaki E. coli kontaminasyonu üzerine etkinlikleri test edilmiştir. Sonuç ve Tartışma: Çalışma sonucunda 30 çevresel su örneğinden 17 E. coli suşu izole edilmiştir. Su örneklerinden aynı zamanda 30 farklı plak yapısında litik bakteriyofaj izolasyonu sağlanmıştır. İzole edilen fajların test edilen bakteriler üzerinde 32-70 aralığında litik etkinliğe sahip oldukları bulunmuştur. Seçilen üç fajın ve bunlardan hazırlanan kokteylin sudaki E. coli kontaminasyonu üzerine etkinliği 6 ve 24. saatte ölçülmüştür. Bunun sonucunda kokteyl uygulamasının sudaki konak bakteri sayısını tespit edilebilen sınırların altına düşürdüğü ayrıca konak olmayan test bakterilerinde 5 log azalma sağladığı ve etkisini 24 saat süresince de koruduğu görülmüştür. Sonuçlar değerlendirildiğinde kokteyl faj uygulamasının sudaki E. coli kontaminasyonuna karşı etkin bir yöntem olacağı düşünülmektedir.

Article Details

THE EFFECT OF ESCHERICHIA COLI BACTERIOPHAGE COCKTAIL ON BACTERIAL CONTAMINATION IN WATER

Authors:

Hilal Basak Erol

Publisher:

Ankara University

Main Topics:

Farmasotik Mikrobiyoloji

Journal:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 1, pp.242-250

Document Type:

Article

Publication Date:

1/2024

Document Language:

English

Document ID (Org No.):

2001300958

pISSN:

1015-3918

Escherichia coli

phage application

phage cocktail

water contaminationEscherichia coli

faj kokteyli

faj uygulaması

su kontaminasyonu

Issues

Article Details

Authors: Hilal Basak Erol

Publisher: Ankara University

Main Topics: Farmasotik Mikrobiyoloji

Journal: Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 1, pp.242-250

Document Type: Article

Publication Date: 1/2024

Document Language: English

Document ID (Org No.): 2001300958

pISSN: 1015-3918

Issues

Authors:

Hilal Basak Erol

Publisher:

Ankara University

Main Topics:

Farmasotik Mikrobiyoloji

Journal:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 1, pp.242-250

Document Type:

Article

Publication Date:

1/2024

Document Language:

English

Document ID (Org No.):

2001300958

pISSN:

1015-3918