Nitrogen use efficiency of Buttum (Pistacia khinjuk Stocks) on MS nutrient medium

Yusuf Ersalı

Article Details

Nitrogen use efficiency of Buttum (Pistacia khinjuk Stocks) on MS nutrient medium

Authors:

Yusuf Ersalı

Publisher:

Mus Alparslan University

Main Topics:

Bitki Biyoteknolojisi

Journal:

Muş Alparslan Üniversitesi Fen Bilimleri Dergisi Vol.12 Issue 1, pp.58-63

Document Type:

Article

Publication Date:

1/2024

Document Language:

Turkish

Document ID (Org No.):

2001317853

pISSN:

2147-7930

Keywords:

Abstract:

 P. khinjuk species is a native rootstock plant species of pistachio. The difficulty of seed and stem cuttings propagation of P. khinjuk make shoot micropropagation a good option for this species. In order to make the micropropagation of this species more efficient and economical, the amount of ammonium nitrate, the types and amount of auxin and cytokinin have been optimized. 1650, 825, 412.5 and 206.25 mgL amounts of NH4NO3 were added to basic nutrient medium of Murashige and Skoog MS to investigated propagation and rooting of shoot of P. khinjuk species., Benzylamino purine BAP, Furfurilaminopurine Kinetin, 2-Isopentyl Adenine 2-IP, for the shoot micropropagation stage, Naphthalene acetic acid NAA, Indole butyric acid IBA and indole acetic acid IAA for the rooting stage of the shoots were used. In shoot micropropagation, the highest number of shoots per explant 1.79, average shoot length 17.10 mm and total soluble protein 3.05 mgg amounts were obtained from MS medium containing 825 mgL NH4NO3 supplemented with 0.5 mgL BAP. In rooting of shoot, the highest value of rooting rate 81, number of roots per explant 4.25, average root length 15.95 mm and total soluble protein amount 3.25 mgg were observed in 0.5 mgL NAA medium containing 412.5 mgL NH4NO3.

 P. khinjuk species is a native rootstock plant species of pistachio. The difficulty of seed and stem cuttings propagation of P. khinjuk make shoot micropropagation a good option for this species. In order to make the micropropagation of this species more efficient and economical, the amount of ammonium nitrate, the types and amount of auxin and cytokinin have been optimized. 1650, 825, 412.5 and 206.25 mgL amounts of NH4NO3 were added to basic nutrient medium of Murashige and Skoog MS to investigated propagation and rooting of shoot of P. khinjuk species., Benzylamino purine BAP, Furfurilaminopurine Kinetin, 2-Isopentyl Adenine 2-IP, for the shoot micropropagation stage, Naphthalene acetic acid NAA, Indole butyric acid IBA and indole acetic acid IAA for the rooting stage of the shoots were used. In shoot micropropagation, the highest number of shoots per explant 1.79, average shoot length 17.10 mm and total soluble protein 3.05 mgg amounts were obtained from MS medium containing 825 mgL NH4NO3 supplemented with 0.5 mgL BAP. In rooting of shoot, the highest value of rooting rate 81, number of roots per explant 4.25, average root length 15.95 mm and total soluble protein amount 3.25 mgg were observed in 0.5 mgL NAA medium containing 412.5 mgL NH4NO3.

 P. khinjuk species is a native rootstock plant species of pistachio. The difficulty of seed and stem cuttings propagation of P. khinjuk make shoot micropropagation a good option for this species. In order to make the micropropagation of this species more efficient and economical, the amount of ammonium nitrate, the types and amount of auxin and cytokinin have been optimized. 1650, 825, 412.5 and 206.25 mgL amounts of NH4NO3 were added to basic nutrient medium of Murashige and Skoog MS to investigated propagation and rooting of shoot of P. khinjuk species., Benzylamino purine BAP, Furfurilaminopurine Kinetin, 2-Isopentyl Adenine 2-IP, for the shoot micropropagation stage, Naphthalene acetic acid NAA, Indole butyric acid IBA and indole acetic acid IAA for the rooting stage of the shoots were used. In shoot micropropagation, the highest number of shoots per explant 1.79, average shoot length 17.10 mm and total soluble protein 3.05 mgg amounts were obtained from MS medium containing 825 mgL NH4NO3 supplemented with 0.5 mgL BAP. In rooting of shoot, the highest value of rooting rate 81, number of roots per explant 4.25, average root length 15.95 mm and total soluble protein amount 3.25 mgg were observed in 0.5 mgL NAA medium containing 412.5 mgL NH4NO3.

 P. khinjuk türü antep fıstığının doğal bir anaç türüdür. P. khinjukun tohum ve gövde çelikleriyle çoğaltılmasının zorluğu, sürgünlerin mikroçoğaltılmasını iyi bir seçenek haline getirmektedir. Bu türün mikroçoğaltımını daha verimli ve ekonomik hale getirmek amacıyla amonyum nitrat miktarı, oksin ve sitokinin çeşitleri ve miktarları optimize edilmiştir. P. khinjuk türünün sürgün ucu kültürü ile mikroçoğaltımında Murashige ve Skoog MS temel besin ortamına NH4NO3ın 1650, 825, 412.5 ve 206.25 mgL düzeylerinde katılmasının sürgün ve kök oluşumu üzerine etkileri araştırılmıştır. Sürgün mikroçoğaltım aşamasında benzilamino pürin BAP, Furfurilaminopurin Kinetin, 2-İzopentil Adenin 2-IP, sürgünlerin köklendirilmesi aşamasında, Naftalen asetik asit NAA, İndol butirik asit IBA ve indol asetik asit IAA kullanılmıştır. Sürgün mikroçoğaltımında en yüksek eksplant başına düşen sürgün sayısı 1.79, ortalama sürgün uzunluğu 17.10 mm ve total çözülebilir protein 3.05 mgg değerinin 0.5 mgL BAP destekli 825 mgL NH4NO3 içeren MS ortamından elde edilmiştir. Sürgünlerin in vitro köklendirilmesinde köklenme oranı  81, eksplant başına düşen kök sayısı 4.25, ortalama kök uzunluğu 15.95 mm ve total çözülebilir protein miktarı 3.25 mgg 412.5 mgL NH4NO3 içeren 0.5 mgL NAA ortamında en yüksek değerde çıkmıştır.

Article Details

Nitrogen use efficiency of Buttum (Pistacia khinjuk Stocks) on MS nutrient medium

Authors:

Yusuf Ersalı

Publisher:

Mus Alparslan University

Main Topics:

Bitki Biyoteknolojisi

Journal:

Muş Alparslan Üniversitesi Fen Bilimleri Dergisi Vol.12 Issue 1, pp.58-63

Document Type:

Article

Publication Date:

1/2024

Document Language:

Turkish

Document ID (Org No.):

2001317853

pISSN:

2147-7930

MS

Ammonium Nitrate

NAA

Soluble ProteinAmonyum nitrat

çözülebilir ptotein

MS

NAA

Issues

Article Details

Authors: Yusuf Ersalı

Publisher: Mus Alparslan University

Main Topics: Bitki Biyoteknolojisi

Journal: Muş Alparslan Üniversitesi Fen Bilimleri Dergisi Vol.12 Issue 1, pp.58-63

Document Type: Article

Publication Date: 1/2024

Document Language: Turkish

Document ID (Org No.): 2001317853

pISSN: 2147-7930

Issues

Authors:

Yusuf Ersalı

Publisher:

Mus Alparslan University

Main Topics:

Bitki Biyoteknolojisi

Journal:

Muş Alparslan Üniversitesi Fen Bilimleri Dergisi Vol.12 Issue 1, pp.58-63

Document Type:

Article

Publication Date:

1/2024

Document Language:

Turkish

Document ID (Org No.):

2001317853

pISSN:

2147-7930