Potential protective effects of silk fibroin against ethanol induced gastric ulcer

Aslı Aykaç; Ahmet Özer Şehirli

Article Details

Potential protective effects of silk fibroin against ethanol induced gastric ulcer

Authors:

Aslı Aykaç | Ahmet Özer Şehirli

Publisher:

Marmara University

Main Topics:

Pharmacology and Pharmaceutical Sciences (Other)

Journal:

Journal of Research in Pharmacy Vol.22 Issue 2, pp.163-172

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001346169

pISSN:

2630-6344

Keywords:

Abstract:

 Ulcers that develop due to alcohol consumption are frequently confronted. The aim of this study was to investigate the protective effects of silk fibroin on the pro-apoptotic and anti-apoptotic protein expression levels on ethanol induced-ulcer models in rats. For three consecutive days, orogastrically either silk fibroin or saline were given to rats. On the 4th day, animals were deprived of food but allowed free access to water for 24 h before the experiment. While control groups were treated with only physiological saline, the ulcer groups were treated orally either with saline or silk fibroin groups. For ulcer induction, 1 ml of absolute ethanol by gavage were given to both group. Firstly, intracardiac blood was taken at 60 min of EtOH or saline administration and immediately animals were decapitated. In blood samples, the amount of TNF-, IL-1β were analysed whereas in stomach tissues, the levels of MDA, GSH, and MPO activity and the expression levels of bcl-2, Bax, caspase-3 and -9 were determined. In ulcer groups, the amount of TNF- , IL-1β, MDA, MPO, caspase-3 and caspase-9 were found to be significantly higher compared to control groups whereas in GSH, Bcl-2Bax were found to be lower p  0.005. In treatment groups it is observed that silk fibroin recovers the amount of TNF-, IL 1-β, MDA, MPO, GSH, caspase-3, caspase-9, and Bcl2Bax. In conclusion, for the treatment of the gastric ulcer SF thought to be very efficient therapeutic agent.

 Ulcers that develop due to alcohol consumption are frequently confronted. The aim of this study was to investigate the protective effects of silk fibroin on the pro-apoptotic and anti-apoptotic protein expression levels on ethanol induced-ulcer models in rats. For three consecutive days, orogastrically either silk fibroin or saline were given to rats. On the 4th day, animals were deprived of food but allowed free access to water for 24 h before the experiment. While control groups were treated with only physiological saline, the ulcer groups were treated orally either with saline or silk fibroin groups. For ulcer induction, 1 ml of absolute ethanol by gavage were given to both group. Firstly, intracardiac blood was taken at 60 min of EtOH or saline administration and immediately animals were decapitated. In blood samples, the amount of TNF-, IL-1β were analysed whereas in stomach tissues, the levels of MDA, GSH, and MPO activity and the expression levels of bcl-2, Bax, caspase-3 and -9 were determined. In ulcer groups, the amount of TNF- , IL-1β, MDA, MPO, caspase-3 and caspase-9 were found to be significantly higher compared to control groups whereas in GSH, Bcl-2Bax were found to be lower p  0.005. In treatment groups it is observed that silk fibroin recovers the amount of TNF-, IL 1-β, MDA, MPO, GSH, caspase-3, caspase-9, and Bcl2Bax. In conclusion, for the treatment of the gastric ulcer SF thought to be very efficient therapeutic agent.

 Ulcers that develop due to alcohol consumption are frequently confronted. The aim of this study was to investigate the protective effects of silk fibroin on the pro-apoptotic and anti-apoptotic protein expression levels on ethanol induced-ulcer models in rats. For three consecutive days, orogastrically either silk fibroin or saline were given to rats. On the 4th day, animals were deprived of food but allowed free access to water for 24 h before the experiment. While control groups were treated with only physiological saline, the ulcer groups were treated orally either with saline or silk fibroin groups. For ulcer induction, 1 ml of absolute ethanol by gavage were given to both group. Firstly, intracardiac blood was taken at 60 min of EtOH or saline administration and immediately animals were decapitated. In blood samples, the amount of TNF-, IL-1β were analysed whereas in stomach tissues, the levels of MDA, GSH, and MPO activity and the expression levels of bcl-2, Bax, caspase-3 and -9 were determined. In ulcer groups, the amount of TNF- , IL-1β, MDA, MPO, caspase-3 and caspase-9 were found to be significantly higher compared to control groups whereas in GSH, Bcl-2Bax were found to be lower p  0.005. In treatment groups it is observed that silk fibroin recovers the amount of TNF-, IL 1-β, MDA, MPO, GSH, caspase-3, caspase-9, and Bcl2Bax. In conclusion, for the treatment of the gastric ulcer SF thought to be very efficient therapeutic agent.

 Ulcers that develop due to alcohol consumption are frequently confronted. The aim of this study was to investigate the protective effects of silk fibroin on the pro-apoptotic and anti-apoptotic protein expression levels on ethanol induced-ulcer models in rats. For three consecutive days, orogastrically either silk fibroin or saline were given to rats. On the 4th day, animals were deprived of food but allowed free access to water for 24 h before the experiment. While control groups were treated with only physiological saline, the ulcer groups were treated orally either with saline or silk fibroin groups. For ulcer induction, 1 ml of absolute ethanol by gavage were given to both group. Firstly, intracardiac blood was taken at 60 min of EtOH or saline administration and immediately animals were decapitated. In blood samples, the amount of TNF-, IL-1β were analysed whereas in stomach tissues, the levels of MDA, GSH, and MPO activity and the expression levels of bcl-2, Bax, caspase-3 and -9 were determined. In ulcer groups, the amount of TNF- , IL-1β, MDA, MPO, caspase-3 and caspase-9 were found to be significantly higher compared to control groups whereas in GSH, Bcl-2Bax were found to be lower p  0.005. In treatment groups it is observed that silk fibroin recovers the amount of TNF-, IL 1-β, MDA, MPO, GSH, caspase-3, caspase-9, and Bcl2Bax. In conclusion, for the treatment of the gastric ulcer SF thought to be very efficient therapeutic agent.

Article Details

Potential protective effects of silk fibroin against ethanol induced gastric ulcer

Authors:

Aslı Aykaç | Ahmet Özer Şehirli

Publisher:

Marmara University

Main Topics:

Pharmacology and Pharmaceutical Sciences (Other)

Journal:

Journal of Research in Pharmacy Vol.22 Issue 2, pp.163-172

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001346169

pISSN:

2630-6344

ethanol

gastric mucosal injury

silk fibroin

apoptosis

cytokines

ulcer

Issues

Article Details

Potential protective effects of silk fibroin against ethanol induced gastric ulcer

Authors: Aslı Aykaç | Ahmet Özer Şehirli

Publisher: Marmara University

Main Topics: Pharmacology and Pharmaceutical Sciences (Other)

Journal: Journal of Research in Pharmacy Vol.22 Issue 2, pp.163-172

Document Type: Article

Publication Date: 6/2025

Document Language: English

Document ID (Org No.): 2001346169

pISSN: 2630-6344

ethanol

gastric mucosal injury

silk fibroin

apoptosis

cytokines

ulcer

Issues

Authors:

Aslı Aykaç | Ahmet Özer Şehirli

Publisher:

Marmara University

Main Topics:

Pharmacology and Pharmaceutical Sciences (Other)

Journal:

Journal of Research in Pharmacy Vol.22 Issue 2, pp.163-172

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001346169

pISSN:

2630-6344