The Association Between the Testis Microbiota and Male Infertility

Ayşe ALTUN; Sibel BULGURCUOGLU KURAN; İlknur KESKİN; Ateş KADIOĞLU

doi:https://doi.org/10.26650/experimed.2021.955321

Article Details

The Association Between the Testis Microbiota and Male Infertility

Authors:

Ayşe ALTUN | Sibel BULGURCUOGLU KURAN | İlknur KESKİN | Ateş KADIOĞLU

Publisher:

Istanbul University

Main Topics:

Diseases

Journal:

Experimed 2021 Vol.11 Issue 2, pp.140-142

Document Type:

Article

Publication Date:

8/2021

Document Language:

English

Document ID (Org No.):

2000428917

eISSN:

2667-5846

DOI:

https://doi.org/10.26650/experimed.2021.955321

Abstract:

 

In recent years, substantial findings have been reported about the effects of microbiota on human health. In our study, we aimed to compare the outcomes of microbiota assays of testicular tissue ob-tained  by  testicular  sperm  extraction  (TESE)  from  2  patients  with  male infertility. In the first patient who had azoospermia, Illumina sequencing detected the following bacterial flora in the collected tissue  homogenates:  Firmicutes  (53%),  Bacteroidetes  (12%),  Ac-tinobacteria  (12%),  Proteobacteria  (8%),  Fusobacteria  (8%),  SR1  (7%),  TM7-3  (3%)  and  OD-1  (1%).  In  the  second  patient  who  had  oligozoospermia,  Firmicutes  (13%),  Bacteroidetes  (15%),  Actino-bacteria  (7%)  and  Proteobacteria  (64%)  were  detected  in  the  tes-ticular  tissue.  Our  preliminary  study  confirmed  that  certain  types  of bacteria exist in testicular tissues of infertile men. Future studies with  more  infertile  and  healthy  men  are  required  to  understand  the  importance  of  these  microorganisms  in  the  pathophysiology  of male infertility.

 


Son  yıllarda  mikrobiyotanın  insan  sağlığı  üzerindeki  etkileri  hak-kında önemli bulgular rapor edilmiştir. Çalışmamızda erkek inferti-litesi olan 2 hastadan testiküler sperm ekstraksiyonu (TESE) ile elde edilen  testis  dokusunun  mikrobiyota  analizinin  sonuçlarını  karşı-laştırmayı amaçladık. Azospermik olan ilk hastada, toplanan doku homojenatlarında  Illumina  dizilemesi  ile  şu  bakteri  florasına  sap-tandı: Firmicutes (%53), Bacteroidetes (%12), Actinobacteria (%12), Proteobacteria  (%8),  Fusobacteria  (%8),  SR1  (%7),  TM7-3  (%3)  ve  OD-1 (%1). Oligozoospermi olan ikinci hastanın ise testis dokusun-da  Firmicutes  (%13),  Bacteroidetes  (%15),  Actinobacteria  (%7)  ve  Proteobacteria (%64) bakterileri saptandı. Ön çalışmamızda, infer-til erkeklerin testis dokusunda belirli bakteri türlerinin bulunduğu doğrulandı.  Erkek  infertilitesinin  patofizyolojisinde  bu  mikroorga-nizmaların önemini anlamak için infertil ve sağlıklı erkeklerle yapı-lacak ileri çalışmalara ihtiyaç vardır.

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Article Details

The Association Between the Testis Microbiota and Male Infertility

Authors: Ayşe ALTUN | Sibel BULGURCUOGLU KURAN | İlknur KESKİN | Ateş KADIOĞLU

Publisher: Istanbul University

Main Topics: Diseases

Journal: Experimed 2021 Vol.11 Issue 2, pp.140-142

Document Type: Article

Publication Date: 8/2021

Document Language: English

Document ID (Org No.): 2000428917

eISSN: 2667-5846

DOI: https://doi.org/10.26650/experimed.2021.955321

Issues

Authors:

Ayşe ALTUN | Sibel BULGURCUOGLU KURAN | İlknur KESKİN | Ateş KADIOĞLU

Publisher:

Istanbul University

Main Topics:

Diseases

Journal:

Experimed 2021 Vol.11 Issue 2, pp.140-142

Document Type:

Article

Publication Date:

8/2021

Document Language:

English

Document ID (Org No.):

2000428917

eISSN:

2667-5846

DOI:

https://doi.org/10.26650/experimed.2021.955321