Oxidized Low-density Lipoprotein Upregulates GM2 Activator Protein Gene Expression

Hidekatsu Yanai; Hiroshi Yoshida; Hironobu Fujiwara; Shigeru Yoshida; Hirotoshi Fuda

doi:10.3844/ajbbsp.2005.90.94

Article Details

Oxidized Low-density Lipoprotein Upregulates GM2 Activator Protein Gene Expression

Authors:

Hidekatsu Yanai | Hiroshi Yoshida | Hironobu Fujiwara | Shigeru Yoshida | Hirotoshi Fuda

Publisher:

Science Publications

Journal:

American Journal of Biochemistry and Biotechnology Vol.1 Issue 2, pp.90-94

Document Type:

Article

Publication Date:

6/2005

Document Language:

English

Document ID (Org No.):

2001396865

pISSN:

1558-6332

DOI:

10.3844/ajbbsp.2005.90.94

Keywords:

Abstract:

 Oxidized low-density lipoprotein (LDL) has been shown to be a powerful regulator of gene expression in monocyte-derived macrophage. To determine the effects of oxidized LDL on macrophage gene expression, macrophages incubated with native or oxidized LDL were analyzed by differential display technique. The differentially expressed cDNA (387 bp) fragment by oxidized LDL showed 100% homology to the part of 3’-untranslated region of the gene of human GM2 activator protein. Quantitative RT-PCR data using specific TaqMan probe showed the increased GM2 activator protein mRNA expression in macrophages incubated with oxidized LDL by about twice (215.4±31.6 %) of that in macrophages incubated with native LDL. Our results shows that ox-LDL upregulates GM2 activator protein gene expression, indicating that GM2 activator protein may be associated with the pathogenesis of atherosclerosis.

Article Details

Oxidized Low-density Lipoprotein Upregulates GM2 Activator Protein Gene Expression

Authors:

Hidekatsu Yanai | Hiroshi Yoshida | Hironobu Fujiwara | Shigeru Yoshida | Hirotoshi Fuda

Publisher:

Science Publications

Journal:

American Journal of Biochemistry and Biotechnology Vol.1 Issue 2, pp.90-94

Document Type:

Article

Publication Date:

6/2005

Document Language:

English

Document ID (Org No.):

2001396865

pISSN:

1558-6332

DOI:

10.3844/ajbbsp.2005.90.94

Oxidized LDL

macrophage

GM2 activator protein

differential display

Issues

Article Details

Oxidized Low-density Lipoprotein Upregulates GM2 Activator Protein Gene Expression

Authors: Hidekatsu Yanai | Hiroshi Yoshida | Hironobu Fujiwara | Shigeru Yoshida | Hirotoshi Fuda

Publisher: Science Publications

Journal: American Journal of Biochemistry and Biotechnology Vol.1 Issue 2, pp.90-94

Document Type: Article

Publication Date: 6/2005

Document Language: English

Document ID (Org No.): 2001396865

pISSN: 1558-6332

DOI: 10.3844/ajbbsp.2005.90.94

Oxidized LDL

macrophage

GM2 activator protein

differential display

Issues

Authors:

Hidekatsu Yanai | Hiroshi Yoshida | Hironobu Fujiwara | Shigeru Yoshida | Hirotoshi Fuda

Publisher:

Science Publications

Journal:

American Journal of Biochemistry and Biotechnology Vol.1 Issue 2, pp.90-94

Document Type:

Article

Publication Date:

6/2005

Document Language:

English

Document ID (Org No.):

2001396865

pISSN:

1558-6332

DOI:

10.3844/ajbbsp.2005.90.94