Investigation of The Effects of Extraction Polarity Change on the Bioactivity of  Eruca vesicaria

Mehmet Emin Uslu

Article Details

Investigation of The Effects of Extraction Polarity Change on the Bioactivity of Eruca vesicaria

Authors:

Mehmet Emin Uslu

Publisher:

Karyay Karadeniz Yayımcılık

Main Topics:

Doğal Ürünler ve Biyoaktif Bileşikler

Journal:

Black Sea Journal of Engineering and Science Vol.7 Issue 6, pp.1287-1293

Document Type:

Article

Publication Date:

11/2024

Document Language:

English

Document ID (Org No.):

2001330292

pISSN:

2619-8991

Keywords:

Abstract:

 Eruca vesicaria arugula plant is frequently used today as a daily food source that contains a rich variety of minerals and vitamins, especially vitamin C. It also contains a high percentage of phenolic compounds which are structures that plants develop to protect themselves from harmful organisms. Phenolic compounds found in plants are obtained by different extraction methods and have a high antioxidant effect. In this study, we report, anticancer activity extract from Eruca vesicaria against human prostate cancer cells PC-3 in vitro. The phenolic substances contained in the plant were obtained in different concentrations by the extraction technique based on the polarity difference. HPLC and total phenol content were determined to perform extraction content analysis. According to in vitro MTT cell proliferation assay, it acted at high concentrations, regardless of polarity differences. The highest cytotoxic effect was observed in extract extracted with 50 ethanol concentration. It has been observed that it has an anticancer effect compared to the determination of total phenol content. Also, because of 24-hour MIC analysis, it shows antibacterial properties according to agent concentration. As a result of this study, it adds many new information to the literature, but also provides guidance for future research.

 Eruca vesicaria arugula plant is frequently used today as a daily food source that contains a rich variety of minerals and vitamins, especially vitamin C. It also contains a high percentage of phenolic compounds which are structures that plants develop to protect themselves from harmful organisms. Phenolic compounds found in plants are obtained by different extraction methods and have a high antioxidant effect. In this study, we report, anticancer activity extract from Eruca vesicaria against human prostate cancer cells PC-3 in vitro. The phenolic substances contained in the plant were obtained in different concentrations by the extraction technique based on the polarity difference. HPLC and total phenol content were determined to perform extraction content analysis. According to in vitro MTT cell proliferation assay, it acted at high concentrations, regardless of polarity differences. The highest cytotoxic effect was observed in extract extracted with 50 ethanol concentration. It has been observed that it has an anticancer effect compared to the determination of total phenol content. Also, because of 24-hour MIC analysis, it shows antibacterial properties according to agent concentration. As a result of this study, it adds many new information to the literature, but also provides guidance for future research.

 Eruca vesicaria arugula plant is frequently used today as a daily food source that contains a rich variety of minerals and vitamins, especially vitamin C. It also contains a high percentage of phenolic compounds which are structures that plants develop to protect themselves from harmful organisms. Phenolic compounds found in plants are obtained by different extraction methods and have a high antioxidant effect. In this study, we report, anticancer activity extract from Eruca vesicaria against human prostate cancer cells PC-3 in vitro. The phenolic substances contained in the plant were obtained in different concentrations by the extraction technique based on the polarity difference. HPLC and total phenol content were determined to perform extraction content analysis. According to in vitro MTT cell proliferation assay, it acted at high concentrations, regardless of polarity differences. The highest cytotoxic effect was observed in extract extracted with 50 ethanol concentration. It has been observed that it has an anticancer effect compared to the determination of total phenol content. Also, because of 24-hour MIC analysis, it shows antibacterial properties according to agent concentration. As a result of this study, it adds many new information to the literature, but also provides guidance for future research.

 Eruca vesicaria arugula plant is frequently used today as a daily food source that contains a rich variety of minerals and vitamins, especially vitamin C. It also contains a high percentage of phenolic compounds which are structures that plants develop to protect themselves from harmful organisms. Phenolic compounds found in plants are obtained by different extraction methods and have a high antioxidant effect. In this study, we report, anticancer activity extract from Eruca vesicaria against human prostate cancer cells PC-3 in vitro. The phenolic substances contained in the plant were obtained in different concentrations by the extraction technique based on the polarity difference. HPLC and total phenol content were determined to perform extraction content analysis. According to in vitro MTT cell proliferation assay, it acted at high concentrations, regardless of polarity differences. The highest cytotoxic effect was observed in extract extracted with 50 ethanol concentration. It has been observed that it has an anticancer effect compared to the determination of total phenol content. Also, because of 24-hour MIC analysis, it shows antibacterial properties according to agent concentration. As a result of this study, it adds many new information to the literature, but also provides guidance for future research.

Article Details

Investigation of The Effects of Extraction Polarity Change on the Bioactivity of Eruca vesicaria

Authors:

Mehmet Emin Uslu

Publisher:

Karyay Karadeniz Yayımcılık

Main Topics:

Doğal Ürünler ve Biyoaktif Bileşikler

Journal:

Black Sea Journal of Engineering and Science Vol.7 Issue 6, pp.1287-1293

Document Type:

Article

Publication Date:

11/2024

Document Language:

English

Document ID (Org No.):

2001330292

pISSN:

2619-8991

Eruca vesicaria

Anticancer

Antibacterial

Phenolic contents

HPLCEruca vesicaria

Anticancer

Antibacterial

Phenolic contents

HPLC

Issues

Article Details

Authors: Mehmet Emin Uslu

Publisher: Karyay Karadeniz Yayımcılık

Main Topics: Doğal Ürünler ve Biyoaktif Bileşikler

Journal: Black Sea Journal of Engineering and Science Vol.7 Issue 6, pp.1287-1293

Document Type: Article

Publication Date: 11/2024

Document Language: English

Document ID (Org No.): 2001330292

pISSN: 2619-8991

Issues

Authors:

Mehmet Emin Uslu

Publisher:

Karyay Karadeniz Yayımcılık

Main Topics:

Doğal Ürünler ve Biyoaktif Bileşikler

Journal:

Black Sea Journal of Engineering and Science Vol.7 Issue 6, pp.1287-1293

Document Type:

Article

Publication Date:

11/2024

Document Language:

English

Document ID (Org No.):

2001330292

pISSN:

2619-8991