Relationship between SIRT1 gene expression level and disease in age-related cataract cases

Nihat Buğra AĞAOĞLU; Nuray VAROL; Saliha Handan YILDIZ; Cem KARAOSMANOĞLU; Rahmi DUMAN; Müjgan Özdemir ERDOĞAN; Mustafa SOLAK

Article Details

Relationship between SIRT1 gene expression level and disease in age-related cataract cases

Authors:

Nihat Buğra AĞAOĞLU | Nuray VAROL | Saliha Handan YILDIZ | Cem KARAOSMANOĞLU | Rahmi DUMAN | Müjgan Özdemir ERDOĞAN | Mustafa SOLAK

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2019 Vol.49 Issue 4, pp.1068-1072

Document Type:

Article

Publication Date:

7/2019

Document Language:

English

Document ID (Org No.):

2000749980

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144

Abstract:

 

Background/aim: Age-related cataract is the most important visual impairment all over the world. Epigenetic modifications, especially overexpression of histone deacetylases, have become the focus of interest for cataract development in recent years. Sirtuin 1 (SIRT1), a class II histone deacetylase and a member of the sirtuin family, is one of the best-characterized histone deacetylases and has a pivotal role in age-related diseases. However, the association of SIRT1 with age-related cataracts has not yet been fully elucidated. Therefore, we aimed to determine the expression of SIRT1 in age-related cataract patients.Materials and methods: Expressions of SIRT1 were evaluated by quantitative polymerase chain reaction (qPCR) in patients and healthy controls. RNA samples were collected from the anterior capsule and peripheral blood samples of age-related cataract patients. Human lens epithelial cell line B3 and peripheral blood samples of healthy subjects were used as controls. Results:  We  determined  that  the  expression  of  SIRT1  in  blood  and  anterior  capsule  samples  increased  significantly  compared  to  the  control group (P < 0.05). Conclusion: The expression level of SIRT1 plays a vital role in the development of age-related cataract and it can be used as a biomarker. Thus, SIRT1 inhibitors can be used in the treatment of age-related cataract disease.

Article Details

Relationship between SIRT1 gene expression level and disease in age-related cataract cases

Authors: Nihat Buğra AĞAOĞLU | Nuray VAROL | Saliha Handan YILDIZ | Cem KARAOSMANOĞLU | Rahmi DUMAN | Müjgan Özdemir ERDOĞAN | Mustafa SOLAK

Publisher: TUBITAK

Journal: Turkish Journal of Medical Sciences 2019 Vol.49 Issue 4, pp.1068-1072

Document Type: Article

Publication Date: 7/2019

Document Language: English

Document ID (Org No.): 2000749980

Frequency: Bimonthly

eISSN: 1303-6165

pISSN: 1300-0144

Issues

Authors:

Nihat Buğra AĞAOĞLU | Nuray VAROL | Saliha Handan YILDIZ | Cem KARAOSMANOĞLU | Rahmi DUMAN | Müjgan Özdemir ERDOĞAN | Mustafa SOLAK

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2019 Vol.49 Issue 4, pp.1068-1072

Document Type:

Article

Publication Date:

7/2019

Document Language:

English

Document ID (Org No.):

2000749980

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144