miR-33 and RIP140 participate in LPS-induced  acute lung injury

Hua LI; Huan HOU; Shuang LIU; Yangyang FENG; Wansi ZHONG; Xiaojuan HU; Nianlong YAN

Article Details

miR-33 and RIP140 participate in LPS-induced acute lung injury

Authors:

Hua LI | Huan HOU | Shuang LIU | Yangyang FENG | Wansi ZHONG | Xiaojuan HU | Nianlong YAN

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2019 Vol.49 Issue 1, pp.422-428

Document Type:

Article

Publication Date:

1/2019

Document Language:

Turkish

Document ID (Org No.):

2000750407

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144

Abstract:

 

Background/aim: Pulmonary microvascular endothelial cells (PMVECs) play a pivotal role in the process of acute lung injury (ALI), which can be induced by lipopolysaccharide (LPS). Numerous reports have indicated that both miR-33 and RIP140 are involved in the inflammatory response in macrophages. In this study, we sought to investigate whether miR-33 and RIP140 participate in ALI induced by LPS.Materials and methods: First, we isolated and identified PMVECs from BALB/c mice. Subsequently, both PMVECs and BALB/c mice were treated with PBS, LPS, or pyrrolidine dithiocarbamate (PDTC) plus LPS and divided into three groups: control (PBS), LPS (LPS), and L+P (LPS plus PDTC) groups. We assessed pathology by hematoxylin and eosin staining, and miR-33 and RIP140 expression levels were examined using quantitative PCR and Western blot analyses.Results: Our results demonstrated that LPS can induce PMVEC injury and ALI and that LPS treatment significantly decreased miR-33 expression compared with controls (P < 0.001, n = 5). On the contrary, RIP140 was markedly overexpressed by LPS treatment (P < 0.001, n = 5). However, this alteration can be inhibited by pretreatment with PDTC before LPS (P < 0.05, n = 5).Conclusion: This study is the first to confirm that both miR-33 and RIP140 participate in LPS-induced PMVEC injury and ALI, which may help uncover the mechanism of ALI.

Article Details

miR-33 and RIP140 participate in LPS-induced acute lung injury

Authors: Hua LI | Huan HOU | Shuang LIU | Yangyang FENG | Wansi ZHONG | Xiaojuan HU | Nianlong YAN

Publisher: TUBITAK

Journal: Turkish Journal of Medical Sciences 2019 Vol.49 Issue 1, pp.422-428

Document Type: Article

Publication Date: 1/2019

Document Language: Turkish

Document ID (Org No.): 2000750407

Frequency: Bimonthly

eISSN: 1303-6165

pISSN: 1300-0144

Issues

Authors:

Hua LI | Huan HOU | Shuang LIU | Yangyang FENG | Wansi ZHONG | Xiaojuan HU | Nianlong YAN

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2019 Vol.49 Issue 1, pp.422-428

Document Type:

Article

Publication Date:

1/2019

Document Language:

Turkish

Document ID (Org No.):

2000750407

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144