Quantitative Expression of Cell-Free Salivary Transcriptomes (IL8, IL1β, And H3F3A) in Oral Squamous Cell Carcinoma and Oral Erosive Lichen Planus

Darya K. Mahmood; Balkees T. Garib; Belal A. Muhammad

Article Details

Quantitative Expression of Cell-Free Salivary Transcriptomes (IL8, IL1β, And H3F3A) in Oral Squamous Cell Carcinoma and Oral Erosive Lichen Planus

Authors:

Darya K. Mahmood | Balkees T. Garib | Belal A. Muhammad

Publisher:

University of Sulaimani

Journal:

Sulaimani Dental Journal Vol.4 Issue 1, pp.14-20

Document Type:

Article

Publication Date:

2/2025

Document Language:

English

Document ID (Org No.):

2001932898

pISSN:

2309-4656

Keywords:

Abstract:

 Objective: Diagnosis of oral SCC at its earliest stage is crucial for increasing survival rate. Salivary transcriptome meetsdemands for noninvasive, accessible, and highly efficient diagnostic medium. This study designed to investigate whetherquantification of cell-free salivary mRNA of certain genes (IL8, IL1β, H3F3A) is an informative diagnostic tool for earlydetection of oral SCC and malignant transformation in erosive oral lichen planus.Methods: The mRNA of salivary transcriptomes of IL8, IL1B, and H3F3A genes were examined in 18 individuals (six oralSCC, six oral lichen planus, and six healthy people). Up to 5 ml of unstimulated saliva was collected from each person. Thetotal RNA was extracted from the cell-free saliva samples and converted to cDNA. The mRNA expression level of the indicatedgenes was quantified by a qRT-PCR technique using specific primers and SyberGreen reagent. The data obtained from theqRT-PCR reaction was analyzed using the comparative CT (2-ΔΔCT) method. Independent t-test was performed to find therelation between variables. P-value < 0.05 was considered significant.Results: The results show that the mRNA expression of all the three indicated genes (IL8, IL1B, and H3F3A) was up-regulatedin all OSCC and erosive oral lichen planus cases in comparison to normal cases.Conclusions: This work demonstrates a significant correlation between cell-free salivary mRNA of certain genes and casesof OSCC and oral lichen planus. These findings suggest that detection of salivary mRNA of certain related genes hold promisesas future biomarkers for accurate and early diagnosis of oral malignancies.

Article Details

Quantitative Expression of Cell-Free Salivary Transcriptomes (IL8, IL1β, And H3F3A) in Oral Squamous Cell Carcinoma and Oral Erosive Lichen Planus

Authors:

Darya K. Mahmood | Balkees T. Garib | Belal A. Muhammad

Publisher:

University of Sulaimani

Journal:

Sulaimani Dental Journal Vol.4 Issue 1, pp.14-20

Document Type:

Article

Publication Date:

2/2025

Document Language:

English

Document ID (Org No.):

2001932898

pISSN:

2309-4656

Salivary transcriptomes

IL8

IL1B

H3F3A

Lichen planus

Issues

Article Details

Quantitative Expression of Cell-Free Salivary Transcriptomes (IL8, IL1β, And H3F3A) in Oral Squamous Cell Carcinoma and Oral Erosive Lichen Planus

Authors: Darya K. Mahmood | Balkees T. Garib | Belal A. Muhammad

Publisher: University of Sulaimani

Journal: Sulaimani Dental Journal Vol.4 Issue 1, pp.14-20

Document Type: Article

Publication Date: 2/2025

Document Language: English

Document ID (Org No.): 2001932898

pISSN: 2309-4656

Salivary transcriptomes

IL8

IL1B

H3F3A

Lichen planus

Issues

Authors:

Darya K. Mahmood | Balkees T. Garib | Belal A. Muhammad

Publisher:

University of Sulaimani

Journal:

Sulaimani Dental Journal Vol.4 Issue 1, pp.14-20

Document Type:

Article

Publication Date:

2/2025

Document Language:

English

Document ID (Org No.):

2001932898

pISSN:

2309-4656