Analysis of the Crude Antigen of Hymenolepis nana from Mice by SDS-PAGE and the Determination of  Specific Antigens in Protein Structure  by Western Blotting

Bahadır GÖNENÇ

Article Details

Analysis of the Crude Antigen of Hymenolepis nana from Mice by SDS-PAGE and the Determination of Specific Antigens in Protein Structure by Western Blotting

Authors:

Bahadır GÖNENÇ

Publisher:

TUBITAK

Journal:

Turkish Journal of Veterinary and Animal Sciences 2002 Vol.26 Issue 5, pp.1067-1071

Document Type:

Article

Publication Date:

4/2002

Document Language:

Turkish

Document ID (Org No.):

2000770507

Frequency:

Bimonthly

eISSN:

1303-6181

pISSN:

1300-0128

Keywords:

Abstract:

 

Protein bands of crude antigens of Hymenolepis nana were determined by SDS-PAGE and Western blotting. Thirty Swiss albino mice were allotted into two groups of 15 each as positive (infected with H. nana) and negative (non-infected with H. nana) groups. The natural infections of H. nana and other helminths were determined by centrifugal flotation of faeces. After bleeding, the mice were necropsied and their guts were examined for H. nana and other intestinal helminths. Sera from mice were tested by Western blotting and the bands obtained from positive and negative groups were compared. The specific protein band for H. nana infection was determined to be 24 kDa.

 

Protein bands of crude antigens of Hymenolepis nana were determined by SDS-PAGE and Western blotting. Thirty Swiss albino mice were allotted into two groups of 15 each as positive (infected with H. nana) and negative (non-infected with H. nana) groups. The natural infections of H. nana and other helminths were determined by centrifugal flotation of faeces. After bleeding, the mice were necropsied and their guts were examined for H. nana and other intestinal helminths. Sera from mice were tested by Western blotting and the bands obtained from positive and negative groups were compared. The specific protein band for H. nana infection was determined to be 24 kDa.

Article Details

Analysis of the Crude Antigen of Hymenolepis nana from Mice by SDS-PAGE and the Determination of Specific Antigens in Protein Structure by Western Blotting

Authors: Bahadır GÖNENÇ

Publisher: TUBITAK

Journal: Turkish Journal of Veterinary and Animal Sciences 2002 Vol.26 Issue 5, pp.1067-1071

Document Type: Article

Publication Date: 4/2002

Document Language: Turkish

Document ID (Org No.): 2000770507

Frequency: Bimonthly

eISSN: 1303-6181

pISSN: 1300-0128

Hymenolepis nana

mice

SDS-PAGE

Western blotting

Issues

Authors:

Bahadır GÖNENÇ

Publisher:

TUBITAK

Journal:

Turkish Journal of Veterinary and Animal Sciences 2002 Vol.26 Issue 5, pp.1067-1071

Document Type:

Article

Publication Date:

4/2002

Document Language:

Turkish

Document ID (Org No.):

2000770507

Frequency:

Bimonthly

eISSN:

1303-6181

pISSN:

1300-0128