Phenolic compounds and antioxidant and antimicrobial properties of Helichrysum species collected from eastern Anatolia, Turkey

Sevil ALBAYRAK; Ahmet AKSOY; Osman SAĞDIÇ; Ümit BUDAK

Article Details

Phenolic compounds and antioxidant and antimicrobial properties of Helichrysum species collected from eastern Anatolia, Turkey

Authors:

Sevil ALBAYRAK | Ahmet AKSOY | Osman SAĞDIÇ | Ümit BUDAK

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 2010 Vol.34 Issue 4, pp.463-473

Document Type:

Article

Publication Date:

3/2010

Document Language:

Turkish

Document ID (Org No.):

2000813025

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152

Keywords:

Abstract:

 

The antioxidant activity of the methanolic extracts of 4 plants from genus Helichrysum Mill. (Asteraceae) [H. arenarium (L.) Moench subsp. erzincanicum Davis & Kupicha, H. arenarium (L.) Moench subsp. rubicundum (C.Koch.) Davis & Kupicha, H. armenium DC. subsp. araxinum (Kirp.) Takht., and H. plicatum DC. subsp. pseudoplicatum (Nab.) Davis & Kupicha] was investigated with 2 complementary test systems, DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging and phosphomolybdenum assay. H. plicatum subsp. pseudoplicatum showed the highest level of total antioxidant activity (161.79 ± 0.3 mg ascorbic acid equivalent/g extract) in the phosphomolybdenum assay. H. arenarium subsp. erzincanicum showed the highest level of free radical scavenging activity, with an IC50 value of 23.03 µg/mL. The total phenolic contents of the extracts ranged from 71.81 to 144.50 mg gallic acid/g dry extract. Chlorogenic acid, apigenin-7-glucoside, and apigenin were found by HPLC analysis to be the main components in the extracts. Antimicrobial activity was investigated with the agar diffusion method. The methanolic extracts had antibacterial activity against Aeromonas hydrophila, Bacillus brevis, B. cereus, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus ATCC 29213. However, no activity was found against Escherichia coli, Morganella morganii, Mycobacterium smegmatis, Proteus mirabilis, Yersinia enterocolitica, or Saccharomyces cerevisiae.

 

The antioxidant activity of the methanolic extracts of 4 plants from genus Helichrysum Mill. (Asteraceae) [H. arenarium (L.) Moench subsp. erzincanicum Davis & Kupicha, H. arenarium (L.) Moench subsp. rubicundum (C.Koch.) Davis & Kupicha, H. armenium DC. subsp. araxinum (Kirp.) Takht., and H. plicatum DC. subsp. pseudoplicatum (Nab.) Davis & Kupicha] was investigated with 2 complementary test systems, DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging and phosphomolybdenum assay. H. plicatum subsp. pseudoplicatum showed the highest level of total antioxidant activity (161.79 ± 0.3 mg ascorbic acid equivalent/g extract) in the phosphomolybdenum assay. H. arenarium subsp. erzincanicum showed the highest level of free radical scavenging activity, with an IC50 value of 23.03 µg/mL. The total phenolic contents of the extracts ranged from 71.81 to 144.50 mg gallic acid/g dry extract. Chlorogenic acid, apigenin-7-glucoside, and apigenin were found by HPLC analysis to be the main components in the extracts. Antimicrobial activity was investigated with the agar diffusion method. The methanolic extracts had antibacterial activity against Aeromonas hydrophila, Bacillus brevis, B. cereus, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus ATCC 29213. However, no activity was found against Escherichia coli, Morganella morganii, Mycobacterium smegmatis, Proteus mirabilis, Yersinia enterocolitica, or Saccharomyces cerevisiae.

Article Details

Phenolic compounds and antioxidant and antimicrobial properties of Helichrysum species collected from eastern Anatolia, Turkey

Authors:

Sevil ALBAYRAK | Ahmet AKSOY | Osman SAĞDIÇ | Ümit BUDAK

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 2010 Vol.34 Issue 4, pp.463-473

Document Type:

Article

Publication Date:

3/2010

Document Language:

Turkish

Document ID (Org No.):

2000813025

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152

Key words: Helichrysum

antimicrobial activity

antioxidant activity

phenolic compounds

DPPH

Issues

Article Details

Phenolic compounds and antioxidant and antimicrobial properties of Helichrysum species collected from eastern Anatolia, Turkey

Authors: Sevil ALBAYRAK | Ahmet AKSOY | Osman SAĞDIÇ | Ümit BUDAK

Publisher: TUBITAK

Journal: Turkish Journal of Biology 2010 Vol.34 Issue 4, pp.463-473

Document Type: Article

Publication Date: 3/2010

Document Language: Turkish

Document ID (Org No.): 2000813025

Frequency: Bimonthly

eISSN: 1303-6092

pISSN: 1300-0152

Key words: Helichrysum

antimicrobial activity

antioxidant activity

phenolic compounds

DPPH

Issues

Authors:

Sevil ALBAYRAK | Ahmet AKSOY | Osman SAĞDIÇ | Ümit BUDAK

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 2010 Vol.34 Issue 4, pp.463-473

Document Type:

Article

Publication Date:

3/2010

Document Language:

Turkish

Document ID (Org No.):

2000813025

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152