The anti- microbial activity of the aquatic, alcoholic and volatile oil extracts of rosa damascena l. Petals

Bushra Ali Kadhim

Article Details

The anti- microbial activity of the aquatic, alcoholic and volatile oil extracts of rosa damascena l. Petals

Authors:

Bushra Ali Kadhim

Publisher:

University of Kirkuk

Journal:

Kirkuk Journal of Science Vol.8 Issue 1, pp.25-43

Document Type:

Article

Publication Date:

3/2013

Document Language:

English

Document ID (Org No.):

2001931653

pISSN:

3005-4788

Keywords:

Abstract:

 The Petals of Rosa damascena L. had used in this research to prepare three types  of extracts which were aquatic, alcoholic and volatile oil . A set of chemical tests were made on these prepared extracts, as well as, using the Fourier Transform Infrared spectrophotometer to detect the active positions in these extracts. The results showed the foundation of some active compounds (Tannines, Alkaloides, Saponins, Flavonoides, Terpens, Steroids and Volatile Oil) in prepared extracts of Rosa damascena L., as well as, the anti-microbial activity of these extracts was tested on eleven types of microorganisms (contain seven types of bacteria and four types of fungi) which were isolated from burns and wound infections. The resulls of stastical analysis by using L.S.D. test at probability level 0.05 had proved that the concentrations 100, 250 mg/ml of aquatic extract had considered as the MIC and MBC for Proteus mirabilis, Escherichia coli and Candida albicans respectively, while the two concentrations 50, 250 mg/ml of aquatic extract had considered as the MIC and MBC for Staphylococcus  aureus , Burkhorderia  mallei and Candida tropicalis  respectively, and about the concern of Proteus  vulgaris and Epidermophyton  floccosum , the concentration 125 mg/ml had inhibited their growth and the concentration 250 mg/ml had killed them . Also the results affirmed there was no affect of aquatic extracts on Pseudomonas aeruginosa and Trichophyton mentagrophytes in all prepared concentrations. The results showed that the two concentrations 16, 32 mg/ml of alcoholic extract were inhibited and killed for Proteus mirabilis and Pseudomonas  flouresence respectively, while the two concentrations 4, 32 mg/ml of the same extract were inhibited and killed for Burkhorderia  mallei, Escherichia coli, Candida albicans, as well as, Candida tropicalis respectively. The results of this research also indicated that the growth of Epidermophyton floccosum , Trichophyton mentagrophytes , Staphylococcus  aureus and Proteus  vulgaris was inhibited and killed by the alcoholic extract at the concentrations (8, 16), (8, 32), (4, 16) and (8, 32) mg/ml respectively. As well as, the results reffered to the inhibitory activity of volatile oily extract on the growth of all microorganisms under study at the concentrations between (1:2) and (1:3), while the killed concentrations between (1:3) and (1:4).

Article Details

The anti- microbial activity of the aquatic, alcoholic and volatile oil extracts of rosa damascena l. Petals

Authors:

Bushra Ali Kadhim

Publisher:

University of Kirkuk

Journal:

Kirkuk Journal of Science Vol.8 Issue 1, pp.25-43

Document Type:

Article

Publication Date:

3/2013

Document Language:

English

Document ID (Org No.):

2001931653

pISSN:

3005-4788

The Petals of Rosa damascene aquatic

alcoholic and volatile oil

chemical test

Issues

Article Details

The anti- microbial activity of the aquatic, alcoholic and volatile oil extracts of rosa damascena l. Petals

Authors: Bushra Ali Kadhim

Publisher: University of Kirkuk

Journal: Kirkuk Journal of Science Vol.8 Issue 1, pp.25-43

Document Type: Article

Publication Date: 3/2013

Document Language: English

Document ID (Org No.): 2001931653

pISSN: 3005-4788

The Petals of Rosa damascene aquatic

alcoholic and volatile oil

chemical test

Issues

Authors:

Bushra Ali Kadhim

Publisher:

University of Kirkuk

Journal:

Kirkuk Journal of Science Vol.8 Issue 1, pp.25-43

Document Type:

Article

Publication Date:

3/2013

Document Language:

English

Document ID (Org No.):

2001931653

pISSN:

3005-4788