Determination of synthetic colorants in cosmetic   products by reversed-phase high-performance liquid  chromatography coupled with diode-array detector

Tulay Oymak; Emrah Dural

Article Details

Determination of synthetic colorants in cosmetic products by reversed-phase high-performance liquid chromatography coupled with diode-array detector

Authors:

Tulay Oymak | Emrah Dural

Publisher:

Marmara University

Main Topics:

Pharmaceutical Toxicology

Journal:

Journal of Research in Pharmacy Vol.23 Issue 6, pp.1048-1059

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001347953

pISSN:

2630-6344

Keywords:

Abstract:

 This study aimed at developing and validating a reversed-phase RP high-performance liquid chromatography HPLC method, for simultaneous determination of five synthetic dyes called tartrazine TRZ, sunset yellow SY, allura red AC AR, brilliant blue FCF BB and erythrosine B EB in cosmetic samples. The separation was performed by a C18 reverse phase analytical column 4.6 x 250 mm, 5 µm at 30C with gradient elution and the mobile phase contained 20 mM ammonium acetate buffer, acetonitrile and methanol. Flow was 1.0 mLmin. Detection wavelengths of diode array detector DAD were set at 420, 480, 510, 634 and 530 nm for TRZ, SY, AR, BB and EB, respectively. The dyes were analysed in 24 min. The limits of detection LOD was  0.18 µgmL. The recovery was between 88.7 and 103.0. Precision was  .7.33 RSD and accuracy was  3.0 RE. It was determined that 7 different cosmetic samples analyzed, consisting of soap, shower gel, eyeshadow, mouthwash, and lip pencil contained synthetic dyes at a concentration of 0.29 to 10.81 mgg.

 This study aimed at developing and validating a reversed-phase RP high-performance liquid chromatography HPLC method, for simultaneous determination of five synthetic dyes called tartrazine TRZ, sunset yellow SY, allura red AC AR, brilliant blue FCF BB and erythrosine B EB in cosmetic samples. The separation was performed by a C18 reverse phase analytical column 4.6 x 250 mm, 5 µm at 30C with gradient elution and the mobile phase contained 20 mM ammonium acetate buffer, acetonitrile and methanol. Flow was 1.0 mLmin. Detection wavelengths of diode array detector DAD were set at 420, 480, 510, 634 and 530 nm for TRZ, SY, AR, BB and EB, respectively. The dyes were analysed in 24 min. The limits of detection LOD was  0.18 µgmL. The recovery was between 88.7 and 103.0. Precision was  .7.33 RSD and accuracy was  3.0 RE. It was determined that 7 different cosmetic samples analyzed, consisting of soap, shower gel, eyeshadow, mouthwash, and lip pencil contained synthetic dyes at a concentration of 0.29 to 10.81 mgg.

 This study aimed at developing and validating a reversed-phase RP high-performance liquid chromatography HPLC method, for simultaneous determination of five synthetic dyes called tartrazine TRZ, sunset yellow SY, allura red AC AR, brilliant blue FCF BB and erythrosine B EB in cosmetic samples. The separation was performed by a C18 reverse phase analytical column 4.6 x 250 mm, 5 µm at 30C with gradient elution and the mobile phase contained 20 mM ammonium acetate buffer, acetonitrile and methanol. Flow was 1.0 mLmin. Detection wavelengths of diode array detector DAD were set at 420, 480, 510, 634 and 530 nm for TRZ, SY, AR, BB and EB, respectively. The dyes were analysed in 24 min. The limits of detection LOD was  0.18 µgmL. The recovery was between 88.7 and 103.0. Precision was  .7.33 RSD and accuracy was  3.0 RE. It was determined that 7 different cosmetic samples analyzed, consisting of soap, shower gel, eyeshadow, mouthwash, and lip pencil contained synthetic dyes at a concentration of 0.29 to 10.81 mgg.

 This study aimed at developing and validating a reversed-phase RP high-performance liquid chromatography HPLC method, for simultaneous determination of five synthetic dyes called tartrazine TRZ, sunset yellow SY, allura red AC AR, brilliant blue FCF BB and erythrosine B EB in cosmetic samples. The separation was performed by a C18 reverse phase analytical column 4.6 x 250 mm, 5 µm at 30C with gradient elution and the mobile phase contained 20 mM ammonium acetate buffer, acetonitrile and methanol. Flow was 1.0 mLmin. Detection wavelengths of diode array detector DAD were set at 420, 480, 510, 634 and 530 nm for TRZ, SY, AR, BB and EB, respectively. The dyes were analysed in 24 min. The limits of detection LOD was  0.18 µgmL. The recovery was between 88.7 and 103.0. Precision was  .7.33 RSD and accuracy was  3.0 RE. It was determined that 7 different cosmetic samples analyzed, consisting of soap, shower gel, eyeshadow, mouthwash, and lip pencil contained synthetic dyes at a concentration of 0.29 to 10.81 mgg.

Article Details

Determination of synthetic colorants in cosmetic products by reversed-phase high-performance liquid chromatography coupled with diode-array detector

Authors:

Tulay Oymak | Emrah Dural

Publisher:

Marmara University

Main Topics:

Pharmaceutical Toxicology

Journal:

Journal of Research in Pharmacy Vol.23 Issue 6, pp.1048-1059

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001347953

pISSN:

2630-6344

Synthetic colorants

method validation

HPLC-DAD

cosmetic

Issues

Article Details

Determination of synthetic colorants in cosmetic products by reversed-phase high-performance liquid chromatography coupled with diode-array detector

Authors: Tulay Oymak | Emrah Dural

Publisher: Marmara University

Main Topics: Pharmaceutical Toxicology

Journal: Journal of Research in Pharmacy Vol.23 Issue 6, pp.1048-1059

Document Type: Article

Publication Date: 6/2025

Document Language: English

Document ID (Org No.): 2001347953

pISSN: 2630-6344

Synthetic colorants

method validation

HPLC-DAD

cosmetic

Issues

Authors:

Tulay Oymak | Emrah Dural

Publisher:

Marmara University

Main Topics:

Pharmaceutical Toxicology

Journal:

Journal of Research in Pharmacy Vol.23 Issue 6, pp.1048-1059

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001347953

pISSN:

2630-6344