Metil paration bileşiğinin paraoksonaz enzimi üzerine inhibisyon etkisinin araştırılması

Nahit GENÇER

Article Details

Metil paration bileşiğinin paraoksonaz enzimi üzerine inhibisyon etkisinin araştırılması

Authors:

Nahit GENÇER

Publisher:

Balıkesir University

Main Topics:

Applied physics | Astronomy and allied sciences

Journal:

Journal of Balıkesir University Institute of Science and Technology 2019 Vol.21 Issue 2, pp.488-493

Document Type:

Article

Publication Date:

6/2019

Document Language:

Turkish

Document ID (Org No.):

2000925091

Frequency:

Bi-Annual

eISSN:

2536-5142

pISSN:

1301-7985

Keywords:

Abstract:

 


In this study, human paraoxonase (PON1) enzyme was purified by using the hydrophobic interaction chromatography technique including ammonium sulfate precipitation and Sepharose-4B-L-tyrosine-1-aminoantresan gel. The purity control of purified PON1 enzyme was performed by SDS polyacrylamide gel electrophoresis. Purification rate was found to be 663. Then, the inhibition effect of methyl parathion on pure enzyme was investigated. The IC50 value of this compound was 1.41 mM.

.

 

Bu çalışmada, amonyum sülfat çöktürmesi ve Sepharose-4B-L-tirosin-1-aminoantresen jelini içeren hidrofobik etkileşim kromatografisi tekniği kullanılarak insan paraoksonaz (PON1) enzimi saflaştırılmıştır. Saflaştırılan PON1 enziminin saflık kontrolü SDS poliakrilamid jel elektroforezi ile yapılmıştır. Saflaştırma oranı 663 olarak bulunmuştur. Daha sonra saf enzim üzerine metil paration zirai ilacının inhibisyon etkisi incelenmiştir. Söz konusu ilacın IC50 değeri 1,41 mM bulunmuştur.

Article Details

Metil paration bileşiğinin paraoksonaz enzimi üzerine inhibisyon etkisinin araştırılması

Authors:

Nahit GENÇER

Publisher:

Balıkesir University

Main Topics:

Applied physics | Astronomy and allied sciences

Journal:

Journal of Balıkesir University Institute of Science and Technology 2019 Vol.21 Issue 2, pp.488-493

Document Type:

Article

Publication Date:

6/2019

Document Language:

Turkish

Document ID (Org No.):

2000925091

Frequency:

Bi-Annual

eISSN:

2536-5142

pISSN:

1301-7985

Paraoksonaz

saflaştırma

metil paration

Issues

Article Details

Metil paration bileşiğinin paraoksonaz enzimi üzerine inhibisyon etkisinin araştırılması

Authors: Nahit GENÇER

Publisher: Balıkesir University

Main Topics: Applied physics | Astronomy and allied sciences

Journal: Journal of Balıkesir University Institute of Science and Technology 2019 Vol.21 Issue 2, pp.488-493

Document Type: Article

Publication Date: 6/2019

Document Language: Turkish

Document ID (Org No.): 2000925091

Frequency: Bi-Annual

eISSN: 2536-5142

pISSN: 1301-7985

Paraoksonaz

saflaştırma

metil paration

Issues

Authors:

Nahit GENÇER

Publisher:

Balıkesir University

Main Topics:

Applied physics | Astronomy and allied sciences

Journal:

Journal of Balıkesir University Institute of Science and Technology 2019 Vol.21 Issue 2, pp.488-493

Document Type:

Article

Publication Date:

6/2019

Document Language:

Turkish

Document ID (Org No.):

2000925091

Frequency:

Bi-Annual

eISSN:

2536-5142

pISSN:

1301-7985