Effects of The Antidepressant Venlafaxine on The Expression of Peroxiredoxin-3 and Peroxiredoxin-5 Against LPS-induced Oxidative Stress in SHSY-5Y Neurons.

Fadime Aydın Köse; Muhammed İsmail Akgül; Ayçe İdil Arap

Article Details

Effects of The Antidepressant Venlafaxine on The Expression of Peroxiredoxin-3 and Peroxiredoxin-5 Against LPS-induced Oxidative Stress in SHSY-5Y Neurons.

Authors:

Fadime Aydın Köse | Muhammed İsmail Akgül | Ayçe İdil Arap

Publisher:

Hacettepe University

Main Topics:

Pharmaceutical Biochemistry

Journal:

Hacettepe University Journal of the Faculty of Pharmacy Issue Special Issue / International Multidisciplinary Symposium on Drug Research and Development, DRD-2023, pp.17-26

Document Type:

Article

Publication Date:

1/2024

Document Language:

English

Document ID (Org No.):

2001323363

pISSN:

2458-8806

Keywords:

Abstract:

 The main objective of this study was to examine the potential neuroprotective properties of venlafaxine, an antidepressant belonging to the serotonin-norepinephrine reuptake inhibitor class. Furthermore, it provides a novel insight into how venlafaxine might interact with peroxiredoxin-3 and -5 enzymes PRDX-3 and PRDX-5, pivotal cellular antioxidant defence system components. For this purpose, human neuroblastoma cells SH-SY5Y were pretreated with venlafaxine 0-100 μM for 12 h, followed by a 4 h LPS exposure 1 μgmL to induce oxidative stress. Cell viability was determined by MTT assay, and ROS generation was assessed by DCFH-DA assay. Protein and mRNA expression levels of PRDX-3 and PRDX-5 were determined by immunoblotting and qRT-PCR, respectively. Based on the results obtained, it was found that the venlafaxine pretreatment led to a notable reduction in intracellular ROS accumulation induced by LPS when compared to the control group p 0.05. In the same manner, it was observed that venlafaxine pre-treatment altered LPS-induced PRDX-3 and PRDX-5 expression in neuronal cells p 0.05. Our findings indicate the involvement of multifunctional PRDX-3 and PRDX-5 enzymes in the antioxidant effect of venlafaxine and suggest that further investigations into this pathway could provide valuable therapeutic contributions.

 The main objective of this study was to examine the potential neuroprotective properties of venlafaxine, an antidepressant belonging to the serotonin-norepinephrine reuptake inhibitor class. Furthermore, it provides a novel insight into how venlafaxine might interact with peroxiredoxin-3 and -5 enzymes PRDX-3 and PRDX-5, pivotal cellular antioxidant defence system components. For this purpose, human neuroblastoma cells SH-SY5Y were pretreated with venlafaxine 0-100 μM for 12 h, followed by a 4 h LPS exposure 1 μgmL to induce oxidative stress. Cell viability was determined by MTT assay, and ROS generation was assessed by DCFH-DA assay. Protein and mRNA expression levels of PRDX-3 and PRDX-5 were determined by immunoblotting and qRT-PCR, respectively. Based on the results obtained, it was found that the venlafaxine pretreatment led to a notable reduction in intracellular ROS accumulation induced by LPS when compared to the control group p 0.05. In the same manner, it was observed that venlafaxine pre-treatment altered LPS-induced PRDX-3 and PRDX-5 expression in neuronal cells p 0.05. Our findings indicate the involvement of multifunctional PRDX-3 and PRDX-5 enzymes in the antioxidant effect of venlafaxine and suggest that further investigations into this pathway could provide valuable therapeutic contributions.

 The main objective of this study was to examine the potential neuroprotective properties of venlafaxine, an antidepressant belonging to the serotonin-norepinephrine reuptake inhibitor class. Furthermore, it provides a novel insight into how venlafaxine might interact with peroxiredoxin-3 and -5 enzymes PRDX-3 and PRDX-5, pivotal cellular antioxidant defence system components. For this purpose, human neuroblastoma cells SH-SY5Y were pretreated with venlafaxine 0-100 μM for 12 h, followed by a 4 h LPS exposure 1 μgmL to induce oxidative stress. Cell viability was determined by MTT assay, and ROS generation was assessed by DCFH-DA assay. Protein and mRNA expression levels of PRDX-3 and PRDX-5 were determined by immunoblotting and qRT-PCR, respectively. Based on the results obtained, it was found that the venlafaxine pretreatment led to a notable reduction in intracellular ROS accumulation induced by LPS when compared to the control group p 0.05. In the same manner, it was observed that venlafaxine pre-treatment altered LPS-induced PRDX-3 and PRDX-5 expression in neuronal cells p 0.05. Our findings indicate the involvement of multifunctional PRDX-3 and PRDX-5 enzymes in the antioxidant effect of venlafaxine and suggest that further investigations into this pathway could provide valuable therapeutic contributions.

 Bu çalışmanın temel amacı, serotonin-norepinefrin geri alım inhibitörü bir antidepresan olan venlafaksinin potansiyel nöroprotektif özelliklerini incelemektir. Ayrıca, venlafaksinin hücresel antioksidan savunma sisteminin önemli bileşenleri olan peroksirodoksin-3 ve -5 PRDX-3 and PRDX-5 enzimleriyle olası etkileşimine dair yeni bir bakış açısı sunmaktadır. Bu amaçla, insan nöroblastoma SHSY-5Y hücreleri, 12 saat venlafaksin 0-100 µM ile muamele edildikten sonra oksidatif stresi indüklemek için 4 saat LPS 1 µgmL ile inkübe edildiler. Hücre canlılığı MTT testi ile belirlendi ve ROS oluşumu DCFH-DA testi ile değerlendirildi. PRDX-3 ve PRDX-5 protein ve mRNA ifade seviyeleri sırasıyla immunoblotlama ve RT-PCR ile belirlendi. Elde edilen sonuçlara göre, hücrelerde kontrol grubuna göre venlafaksin uygulamasın LPS ile indüklenen hücresel ROT birikiminde belirgin bir azalmaya yol açtığı tespit edilmiştir p 0.05. Benzer olarak, venlafaksin ön işlemi, nöronal hücrelerde LPS tarafından indüklenen PRDX-3 ve PRDX-5 ifadesini değiştirdiği gözlenmiştir p 0.05. Elde edilen bu bulgular, venlafaksinin antioksidan etkisinde multifonksiyonel PRDX-3 ve PRDX-5 enzimlerinin rol oynadığına işaret etmekte olup bu yolak ile ilişkili yapılacak ileri çalışmaların önemli terapötik katkılarda bulunabileceğini düşündürmektedir.

Article Details

Effects of The Antidepressant Venlafaxine on The Expression of Peroxiredoxin-3 and Peroxiredoxin-5 Against LPS-induced Oxidative Stress in SHSY-5Y Neurons.

Authors:

Fadime Aydın Köse | Muhammed İsmail Akgül | Ayçe İdil Arap

Publisher:

Hacettepe University

Main Topics:

Pharmaceutical Biochemistry

Journal:

Hacettepe University Journal of the Faculty of Pharmacy Issue Special Issue / International Multidisciplinary Symposium on Drug Research and Development, DRD-2023, pp.17-26

Document Type:

Article

Publication Date:

1/2024

Document Language:

English

Document ID (Org No.):

2001323363

pISSN:

2458-8806

Venlafaxine

Peroxiredoxin-3

Peroxiredoxin-5

Oxidative Stress

AntioxidantVenlafaksin

Peroksiredoksin-3

Peroksiredoksin-5

Oksidatif stres

Antioksidan

Issues

Article Details

Authors: Fadime Aydın Köse | Muhammed İsmail Akgül | Ayçe İdil Arap

Publisher: Hacettepe University

Main Topics: Pharmaceutical Biochemistry

Journal: Hacettepe University Journal of the Faculty of Pharmacy Issue Special Issue / International Multidisciplinary Symposium on Drug Research and Development, DRD-2023, pp.17-26

Document Type: Article

Publication Date: 1/2024

Document Language: English

Document ID (Org No.): 2001323363

pISSN: 2458-8806

Issues

Authors:

Fadime Aydın Köse | Muhammed İsmail Akgül | Ayçe İdil Arap

Publisher:

Hacettepe University

Main Topics:

Pharmaceutical Biochemistry

Journal:

Hacettepe University Journal of the Faculty of Pharmacy Issue Special Issue / International Multidisciplinary Symposium on Drug Research and Development, DRD-2023, pp.17-26

Document Type:

Article

Publication Date:

1/2024

Document Language:

English

Document ID (Org No.):

2001323363

pISSN:

2458-8806