Advances in Menaquinone-7 Production by Bacillus subtilis Natto: Fed-Batch Glycerol Addition

Aydin Berenjian; Raja Mahanama; Andrea Talbot; Hubert Regtop; John Kavanagh; Fariba Dehghani

doi:10.3844/ajbbsp.2012.105.110

Article Details

Advances in Menaquinone-7 Production by Bacillus subtilis Natto: Fed-Batch Glycerol Addition

Authors:

Aydin Berenjian | Raja Mahanama | Andrea Talbot | Hubert Regtop | John Kavanagh | Fariba Dehghani

Publisher:

Science Publications

Journal:

American Journal of Biochemistry and Biotechnology Vol.8 Issue 2, pp.105-110

Document Type:

Article

Publication Date:

6/2012

Document Language:

English

Document ID (Org No.):

2001397095

pISSN:

1558-6332

DOI:

10.3844/ajbbsp.2012.105.110

Keywords:

Abstract:

 Menaquinone-7 was produced by fermentation mainly using Bacillus subtilis species. Due to the low concentration of menaquinone-7 in fermentation processes and the associated expense of recovering the menaquinone-7, it was desirable to improve the fermentation concentration. The aim of this study was to determine the effect of fed-batch glycerol addition in the production of menaquinone-7 during the fermentation of Bacillus subtilis natto in both small (25-mL) and bench scale (3-L) fermenters. Liquid phase fermentation was carried out aerobically at 40°C for a period of 6 days. The pH, bacterial growth, glycerol and menaquinone-7 concentrations were measured every day. Maximum menaquinone-7 was produced when 2% (w/v) glycerol was added to the fermentation media in the second day (48 h) of fermentation. The results were demonstrated 40% increase of menaquinone-7 concentration compared to batch culture. This study provides valuable data for further optimization and scale-up the menaquinone-7 fermentation process.

Article Details

Advances in Menaquinone-7 Production by Bacillus subtilis Natto: Fed-Batch Glycerol Addition

Authors: Aydin Berenjian | Raja Mahanama | Andrea Talbot | Hubert Regtop | John Kavanagh | Fariba Dehghani

Publisher: Science Publications

Journal: American Journal of Biochemistry and Biotechnology Vol.8 Issue 2, pp.105-110

Document Type: Article

Publication Date: 6/2012

Document Language: English

Document ID (Org No.): 2001397095

pISSN: 1558-6332

DOI: 10.3844/ajbbsp.2012.105.110

Menaquinone-7

glycerol

fed-batch

fermentation

Bacillus subtilis natto

Issues

Authors:

Aydin Berenjian | Raja Mahanama | Andrea Talbot | Hubert Regtop | John Kavanagh | Fariba Dehghani

Publisher:

Science Publications

Journal:

American Journal of Biochemistry and Biotechnology Vol.8 Issue 2, pp.105-110

Document Type:

Article

Publication Date:

6/2012

Document Language:

English

Document ID (Org No.):

2001397095

pISSN:

1558-6332

DOI:

10.3844/ajbbsp.2012.105.110