Effect of keratinocyte growth factor on growth and transdifferentiation  of primary alveolar epithelial type II cells

Xiuxiang LIU; ZHICHUN FENG; Chunmei TIAN; Xiangyong KONG; YUMEI WU; Changjun LV

Article Details

Effect of keratinocyte growth factor on growth and transdifferentiation of primary alveolar epithelial type II cells

Authors:

Xiuxiang LIU | ZHICHUN FENG | Chunmei TIAN | Xiangyong KONG | YUMEI WU | Changjun LV

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2015 Vol.45 Issue 2, pp.251-260

Document Type:

Article

Publication Date:

8/2015

Document Language:

Turkish

Document ID (Org No.):

2000752748

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144

Keywords:

Abstract:

 

Background/aim: To investigate the effects of keratinocyte growth factor (KGF) on the growth and transdifferentiation of primary alveolar epithelial type II cells (AECIIs).   Materials and methods: The number of primary AECIIs, their viability, and cell cycle and apoptosis were studied under KGF treatment using a hemocytometer, trypan blue exclusion, and flow cytometry, respectively. Positive expressions of surfactant protein C (SP-C), aquaporin 5 (AQP5), and thyroid transcription factor 1(TTF-1) were examined with indirect immunofluorescence; mRNA levels of SP-C, AQP5, and TTF-1 were determined by polymerase chain reaction.   Results: In response to KGF treatment, cell numbers were significantly increased, more cells were blocked in the S phase, and fewer cells were apoptotic or necrotic on days 2 and 4, but there was little effect on cell viability. In addition, KGF treatment resulted in higher levels of SP-C on days 2 and 8 while lowering them on day 4, higher levels of AQP5 on day 4 while lowering them on day 8, and higher levels of TTF-1 on days 2, 6, and 8.   Conclusion: KGF treatment promoted proliferation of AECIIs, inhibited cell apoptosis, promoted transdifferentiation of AECIIs, and induced alveolar epithelial type I cells to revert to AECIIs.

 

Background/aim: To investigate the effects of keratinocyte growth factor (KGF) on the growth and transdifferentiation of primary alveolar epithelial type II cells (AECIIs).   Materials and methods: The number of primary AECIIs, their viability, and cell cycle and apoptosis were studied under KGF treatment using a hemocytometer, trypan blue exclusion, and flow cytometry, respectively. Positive expressions of surfactant protein C (SP-C), aquaporin 5 (AQP5), and thyroid transcription factor 1(TTF-1) were examined with indirect immunofluorescence; mRNA levels of SP-C, AQP5, and TTF-1 were determined by polymerase chain reaction.   Results: In response to KGF treatment, cell numbers were significantly increased, more cells were blocked in the S phase, and fewer cells were apoptotic or necrotic on days 2 and 4, but there was little effect on cell viability. In addition, KGF treatment resulted in higher levels of SP-C on days 2 and 8 while lowering them on day 4, higher levels of AQP5 on day 4 while lowering them on day 8, and higher levels of TTF-1 on days 2, 6, and 8.   Conclusion: KGF treatment promoted proliferation of AECIIs, inhibited cell apoptosis, promoted transdifferentiation of AECIIs, and induced alveolar epithelial type I cells to revert to AECIIs.

Article Details

Effect of keratinocyte growth factor on growth and transdifferentiation of primary alveolar epithelial type II cells

Authors:

Xiuxiang LIU | ZHICHUN FENG | Chunmei TIAN | Xiangyong KONG | YUMEI WU | Changjun LV

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2015 Vol.45 Issue 2, pp.251-260

Document Type:

Article

Publication Date:

8/2015

Document Language:

Turkish

Document ID (Org No.):

2000752748

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144

Keratinocyte growth factor

alveolar epithelial type II cells

transdifferentiation

surfactant protein C

aquaporin 5

thyroid transcription factor-1

Issues

Article Details

Effect of keratinocyte growth factor on growth and transdifferentiation of primary alveolar epithelial type II cells

Authors: Xiuxiang LIU | ZHICHUN FENG | Chunmei TIAN | Xiangyong KONG | YUMEI WU | Changjun LV

Publisher: TUBITAK

Journal: Turkish Journal of Medical Sciences 2015 Vol.45 Issue 2, pp.251-260

Document Type: Article

Publication Date: 8/2015

Document Language: Turkish

Document ID (Org No.): 2000752748

Frequency: Bimonthly

eISSN: 1303-6165

pISSN: 1300-0144

Keratinocyte growth factor

alveolar epithelial type II cells

transdifferentiation

surfactant protein C

aquaporin 5

thyroid transcription factor-1

Issues

Authors:

Xiuxiang LIU | ZHICHUN FENG | Chunmei TIAN | Xiangyong KONG | YUMEI WU | Changjun LV

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2015 Vol.45 Issue 2, pp.251-260

Document Type:

Article

Publication Date:

8/2015

Document Language:

Turkish

Document ID (Org No.):

2000752748

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144