Study of the Optimal Conditions of Levan Production from a Local Isolate of Bacillus subtilis subsp. subtilis w36

Wafaa H.  Khassaf; Alaa K.  Niamah; Alaa J. A.  Al-Manhel

doi:10.37077/25200860.2019.211

Article Details

Study of the Optimal Conditions of Levan Production from a Local Isolate of Bacillus subtilis subsp. subtilis w36

Authors:

Wafaa H. Khassaf | Alaa K. Niamah | Alaa J. A. Al-Manhel

Publisher:

College of Agriculture, University of Basrah, Iraq

Main Topics:

Optimal conditions

Journal:

Basrah Journal of Agricultural Sciences Vol.32 Issue 2, pp.213-222

Document Type:

Article

Publication Date:

12/2019

Document Language:

English

Document ID (Org No.):

2001363470

pISSN:

2520-0860

DOI:

10.37077/25200860.2019.211

Keywords:

Abstract:

 The present study was aimed to test optimum conditions for the levan production from local isolation Bacillus subtilis subsp. subtilis w36, which was isolated from Basrah city soil by using production medium containing (40 g of sucrose as carbon source 10g pepton, 1g (NH4) 2SO4, 1g KH2PO4, 1g MgSO4.7H2O). The amount of levan produced was 4.9 g.ml-1. Molasses and date juice were used as substitutes for sucrose in growth media and substitute percentages were (25%, 50%, 75% and100%). The results showed the highest production of levan was recorded when sucrose was substituted with 25% molasses, which was 5.2 g.ml-1.Therefore, this medium was used to study optimum conditions included incubation period, inoculation volume, temperature ,initial pH. The highest production of levan was 6.8 g .100 ml-1 within 40 hours' incubation, 1 ml inoculum volume, pH 6.5 and 32 ºC.

Article Details

Study of the Optimal Conditions of Levan Production from a Local Isolate of Bacillus subtilis subsp. subtilis w36

Authors: Wafaa H. Khassaf | Alaa K. Niamah | Alaa J. A. Al-Manhel

Publisher: College of Agriculture, University of Basrah, Iraq

Main Topics: Optimal conditions

Journal: Basrah Journal of Agricultural Sciences Vol.32 Issue 2, pp.213-222

Document Type: Article

Publication Date: 12/2019

Document Language: English

Document ID (Org No.): 2001363470

pISSN: 2520-0860

DOI: 10.37077/25200860.2019.211

Bacillus subtilis

Molasses

Levan

Production

Optimal conditions

Issues

Authors:

Wafaa H. Khassaf | Alaa K. Niamah | Alaa J. A. Al-Manhel

Publisher:

College of Agriculture, University of Basrah, Iraq

Main Topics:

Optimal conditions

Journal:

Basrah Journal of Agricultural Sciences Vol.32 Issue 2, pp.213-222

Document Type:

Article

Publication Date:

12/2019

Document Language:

English

Document ID (Org No.):

2001363470

pISSN:

2520-0860

DOI:

10.37077/25200860.2019.211