Comparison of Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry with VITEK2 Gram positive system for identification of beta hemolytic Streptococci

Yeşim BEŞLİ

doi:https://doi.org/10.5455/jicm.29.20171123

Article Details

Comparison of Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry with VITEK2 Gram positive system for identification of beta hemolytic Streptococci

Authors:

Yeşim BEŞLİ

Publisher:

Erkan YULA

Main Topics:

Diseases

Journal:

Journal of Immunology and Clinical Microbiology 2018 Vol.3 Issue 1, pp.1-7

Document Type:

Article

Publication Date:

3/2018

Document Language:

English

Document ID (Org No.):

2000740942

Frequency:

Quarterly

eISSN:

2528-9470

DOI:

https://doi.org/10.5455/jicm.29.20171123

Abstract:

 


Background: Matrix–assisted
laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is
known as a successful tool for bacterial identification and suitable for
clinical diagnostics. The aim of our study was to evaluate diagnostic accuracy
of MALDI-TOF MS system and VITEK2 Gram Positive Identification (GP ID) system
for the identification of Beta Hemolytic Streptococci (BHS).


Materials and Methods: A total 148 BHS which were isolated from various clinical
specimens were included in the study. Isolates were identified by Bruker
Biotyper 3.4 and VITEK2 GP ID coupled to the agglutination test. Identification
results for Bruker Biotyper 3.4 and VITEK2 GP ID systems were classified in
three different categories as follows: correct identification, low level of
discrimination, no identification/misidentification. Identification results of
BHS were evaluated according to these categories.


Results: BHS
isolates were defined as Streptococcus pyogenes (n: 69), Streptococcus agalactiae (n: 59), Streptococcus dysgalactiae serogroup C (n: 5), and Streptococcus dysgalactiae serogroup G
(n: 15) according to latex agglutination assay. MALDI-TOF MS analysis of 148
BHS isolates yielded correct identification for 89.9% of S. pyogenes, 64.4% of S.
agalactiae, and 95.0% S.
dysagalactiae serogroup C and G; while VITEK2 GP ID gave correct
identification for 62.3% of S. pyogenes,
67.8% of S. agalactiae, and 65.0% of S. dysagalactiae serogroup C and G.


Conclusions:
MALDI-TOF MS-based identiﬁcation provides faster and more accurate
identiﬁcation of beta-hemolytic streptococcal species than VITEK2 GP ID system.


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Article Details

Comparison of Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry with VITEK2 Gram positive system for identification of beta hemolytic Streptococci

Authors: Yeşim BEŞLİ

Publisher: Erkan YULA

Main Topics: Diseases

Journal: Journal of Immunology and Clinical Microbiology 2018 Vol.3 Issue 1, pp.1-7

Document Type: Article

Publication Date: 3/2018

Document Language: English

Document ID (Org No.): 2000740942

Frequency: Quarterly

eISSN: 2528-9470

DOI: https://doi.org/10.5455/jicm.29.20171123

Issues

Authors:

Yeşim BEŞLİ

Publisher:

Erkan YULA

Main Topics:

Diseases

Journal:

Journal of Immunology and Clinical Microbiology 2018 Vol.3 Issue 1, pp.1-7

Document Type:

Article

Publication Date:

3/2018

Document Language:

English

Document ID (Org No.):

2000740942

Frequency:

Quarterly

eISSN:

2528-9470

DOI:

https://doi.org/10.5455/jicm.29.20171123