Cell Suspension Cultures and High Frequency Shoot Regeneration of Some Hypericum species

Hussein Abdullah Ahmed Ahmed; Serkan Uranbey

Article Details

Cell Suspension Cultures and High Frequency Shoot Regeneration of Some Hypericum species

Authors:

Hussein Abdullah Ahmed Ahmed | Serkan Uranbey

Publisher:

Ankara University

Main Topics:

Plant Biotechnology

Journal:

Journal of Agricultural Sciences Vol.31 Issue 2, pp.319-331

Document Type:

Article

Publication Date:

3/2025

Document Language:

English

Document ID (Org No.):

2001342999

pISSN:

1300-7580

Keywords:

Abstract:

 The Hypericum genus is significant both medically and economically due to its bioactive compounds. This study utilized plant biotechnology techniques to develop an efficient and reliable adventitious shoot regeneration and suitable cell suspension culture system for various Hypericum species, including Hypericum perforatum L., Hypericum leptophyllum Hochst, Hypericum heterophyllum L., Hypericum humifusum L., and Hypericum athoum Boiss.  Orph. High frequency of callus induction using leaf explants on MS medium containing 1.0 mgL BAP and 0.1 mgL 2,4-D for Hypericum perforatum L. 100, Hypericum humifusum L. 90, and Hypericum leptophyllum Hochst 90. The best shoot regeneration was also achieved in Hypericum humifusum L. 86.3, Hypericum perforatum L. 73.0, Hypericum leptophyllum Hochst 45.67 and Hypericum athoum Boiss.  Orph. 18.33 on MS medium with 1.0 mgL BAP and 0.1 mgL 2,4-D. Calli clusters obtained on callus induction medium were cultured on cell suspension culture MS basal media with B5 vitamins containing 2 mgL glycine, 1 mgL 2,4-D, 0.1 mgL KIN, and 0.1 mgL BAP. The friable calli developed in the suspension culture and plated on the medium. The proembryogenic structures formed turned to embryonic structures. These globular embryos further transformed into heart and cotyledonary stage and germinated. The maximum number of shootcallus or percentage of germination of somatic embryo were respectively recorded for H. perforatum L. 15.37callus, H. leptophyllum Hochst 6.9callus, H. heterophyllum L. 9.6callus, H. humifusum L. 16.8callus and H. athoum Boiss.  Orph. 11.7callus. H. perforatum L. and H. humifusum L. showed the best shoot regeneration capacity as recorded in adventitious shoot regeneration studies. The plants were acclimatized with 85-100  survival rat, H. perforatum and H. humifusum L. had also the highest survival rate 100 as having regeneration capacity.

 The Hypericum genus is significant both medically and economically due to its bioactive compounds. This study utilized plant biotechnology techniques to develop an efficient and reliable adventitious shoot regeneration and suitable cell suspension culture system for various Hypericum species, including Hypericum perforatum L., Hypericum leptophyllum Hochst, Hypericum heterophyllum L., Hypericum humifusum L., and Hypericum athoum Boiss.  Orph. High frequency of callus induction using leaf explants on MS medium containing 1.0 mgL BAP and 0.1 mgL 2,4-D for Hypericum perforatum L. 100, Hypericum humifusum L. 90, and Hypericum leptophyllum Hochst 90. The best shoot regeneration was also achieved in Hypericum humifusum L. 86.3, Hypericum perforatum L. 73.0, Hypericum leptophyllum Hochst 45.67 and Hypericum athoum Boiss.  Orph. 18.33 on MS medium with 1.0 mgL BAP and 0.1 mgL 2,4-D. Calli clusters obtained on callus induction medium were cultured on cell suspension culture MS basal media with B5 vitamins containing 2 mgL glycine, 1 mgL 2,4-D, 0.1 mgL KIN, and 0.1 mgL BAP. The friable calli developed in the suspension culture and plated on the medium. The proembryogenic structures formed turned to embryonic structures. These globular embryos further transformed into heart and cotyledonary stage and germinated. The maximum number of shootcallus or percentage of germination of somatic embryo were respectively recorded for H. perforatum L. 15.37callus, H. leptophyllum Hochst 6.9callus, H. heterophyllum L. 9.6callus, H. humifusum L. 16.8callus and H. athoum Boiss.  Orph. 11.7callus. H. perforatum L. and H. humifusum L. showed the best shoot regeneration capacity as recorded in adventitious shoot regeneration studies. The plants were acclimatized with 85-100  survival rat, H. perforatum and H. humifusum L. had also the highest survival rate 100 as having regeneration capacity.

 The Hypericum genus is significant both medically and economically due to its bioactive compounds. This study utilized plant biotechnology techniques to develop an efficient and reliable adventitious shoot regeneration and suitable cell suspension culture system for various Hypericum species, including Hypericum perforatum L., Hypericum leptophyllum Hochst, Hypericum heterophyllum L., Hypericum humifusum L., and Hypericum athoum Boiss.  Orph. High frequency of callus induction using leaf explants on MS medium containing 1.0 mgL BAP and 0.1 mgL 2,4-D for Hypericum perforatum L. 100, Hypericum humifusum L. 90, and Hypericum leptophyllum Hochst 90. The best shoot regeneration was also achieved in Hypericum humifusum L. 86.3, Hypericum perforatum L. 73.0, Hypericum leptophyllum Hochst 45.67 and Hypericum athoum Boiss.  Orph. 18.33 on MS medium with 1.0 mgL BAP and 0.1 mgL 2,4-D. Calli clusters obtained on callus induction medium were cultured on cell suspension culture MS basal media with B5 vitamins containing 2 mgL glycine, 1 mgL 2,4-D, 0.1 mgL KIN, and 0.1 mgL BAP. The friable calli developed in the suspension culture and plated on the medium. The proembryogenic structures formed turned to embryonic structures. These globular embryos further transformed into heart and cotyledonary stage and germinated. The maximum number of shootcallus or percentage of germination of somatic embryo were respectively recorded for H. perforatum L. 15.37callus, H. leptophyllum Hochst 6.9callus, H. heterophyllum L. 9.6callus, H. humifusum L. 16.8callus and H. athoum Boiss.  Orph. 11.7callus. H. perforatum L. and H. humifusum L. showed the best shoot regeneration capacity as recorded in adventitious shoot regeneration studies. The plants were acclimatized with 85-100  survival rat, H. perforatum and H. humifusum L. had also the highest survival rate 100 as having regeneration capacity.

 The Hypericum genus is significant both medically and economically due to its bioactive compounds. This study utilized plant biotechnology techniques to develop an efficient and reliable adventitious shoot regeneration and suitable cell suspension culture system for various Hypericum species, including Hypericum perforatum L., Hypericum leptophyllum Hochst, Hypericum heterophyllum L., Hypericum humifusum L., and Hypericum athoum Boiss.  Orph. High frequency of callus induction using leaf explants on MS medium containing 1.0 mgL BAP and 0.1 mgL 2,4-D for Hypericum perforatum L. 100, Hypericum humifusum L. 90, and Hypericum leptophyllum Hochst 90. The best shoot regeneration was also achieved in Hypericum humifusum L. 86.3, Hypericum perforatum L. 73.0, Hypericum leptophyllum Hochst 45.67 and Hypericum athoum Boiss.  Orph. 18.33 on MS medium with 1.0 mgL BAP and 0.1 mgL 2,4-D. Calli clusters obtained on callus induction medium were cultured on cell suspension culture MS basal media with B5 vitamins containing 2 mgL glycine, 1 mgL 2,4-D, 0.1 mgL KIN, and 0.1 mgL BAP. The friable calli developed in the suspension culture and plated on the medium. The proembryogenic structures formed turned to embryonic structures. These globular embryos further transformed into heart and cotyledonary stage and germinated. The maximum number of shootcallus or percentage of germination of somatic embryo were respectively recorded for H. perforatum L. 15.37callus, H. leptophyllum Hochst 6.9callus, H. heterophyllum L. 9.6callus, H. humifusum L. 16.8callus and H. athoum Boiss.  Orph. 11.7callus. H. perforatum L. and H. humifusum L. showed the best shoot regeneration capacity as recorded in adventitious shoot regeneration studies. The plants were acclimatized with 85-100  survival rat, H. perforatum and H. humifusum L. had also the highest survival rate 100 as having regeneration capacity.

Article Details

Cell Suspension Cultures and High Frequency Shoot Regeneration of Some Hypericum species

Authors:

Hussein Abdullah Ahmed Ahmed | Serkan Uranbey

Publisher:

Ankara University

Main Topics:

Plant Biotechnology

Journal:

Journal of Agricultural Sciences Vol.31 Issue 2, pp.319-331

Document Type:

Article

Publication Date:

3/2025

Document Language:

English

Document ID (Org No.):

2001342999

pISSN:

1300-7580

Cell suspension culture

Shoot regeneration

Hypericum species

Medicinal plant

Issues

Article Details

Cell Suspension Cultures and High Frequency Shoot Regeneration of Some Hypericum species

Authors: Hussein Abdullah Ahmed Ahmed | Serkan Uranbey

Publisher: Ankara University

Main Topics: Plant Biotechnology

Journal: Journal of Agricultural Sciences Vol.31 Issue 2, pp.319-331

Document Type: Article

Publication Date: 3/2025

Document Language: English

Document ID (Org No.): 2001342999

pISSN: 1300-7580

Cell suspension culture

Shoot regeneration

Hypericum species

Medicinal plant

Issues

Authors:

Hussein Abdullah Ahmed Ahmed | Serkan Uranbey

Publisher:

Ankara University

Main Topics:

Plant Biotechnology

Journal:

Journal of Agricultural Sciences Vol.31 Issue 2, pp.319-331

Document Type:

Article

Publication Date:

3/2025

Document Language:

English

Document ID (Org No.):

2001342999

pISSN:

1300-7580