Use of Quinolones in Bull Semen Extenders to Reduce Sperm Deoxyribonucleic Acid Damage

Clara Gonzalez-Marin; Michael Eduard Kjelland; Rosa Roy; Carmen Lopez-Fernandez; Jose Luis Fernandez; Juan Fernando Moreno; Jaime Gosalvez

doi:10.3844/ajavsp.2012.180.185

Article Details

Use of Quinolones in Bull Semen Extenders to Reduce Sperm Deoxyribonucleic Acid Damage

Authors:

Clara Gonzalez-Marin | Michael Eduard Kjelland | Rosa Roy | Carmen Lopez-Fernandez | Jose Luis Fernandez | Juan Fernando Moreno | Jaime Gosalvez

Publisher:

Science Publications

Journal:

American Journal of Animal and Veterinary Sciences Vol.7 Issue 4, pp.180-185

Document Type:

Article

Publication Date:

12/2012

Document Language:

English

Document ID (Org No.):

2001396356

pISSN:

1557-4563

DOI:

10.3844/ajavsp.2012.180.185

Keywords:

Abstract:

 Cryopreserved sperm samples from Holstein bulls (n = 20) were examined for bacterial presence and Sperm DNA Fragmentation (SDF) dynamics. SDF was assessed after thawing (T0) and at 4, 24 and 48 h of incubation (37°C) and the rate of SDF (r-SDF), as an estimator of the DNA degradation over time, was calculated. Two groups of bulls were identified based on the presence or absence of bacteria: One group (n = 10) had a readily detectable bacterial presence, while the other group (n = 10) had an undetectable bacterial presence. Differences in the SDF at T0 were not observed between these two groups. However, statistically different results were found after 24 h of incubation at 37°C (Kaplan-Meier estimator; Log-Rank Matel-Cox, p0.05) were not detected between the control and the quinolone treated sample for those samples without bacteria. However, differences (p.

Article Details

Use of Quinolones in Bull Semen Extenders to Reduce Sperm Deoxyribonucleic Acid Damage

Authors:

Clara Gonzalez-Marin | Michael Eduard Kjelland | Rosa Roy | Carmen Lopez-Fernandez | Jose Luis Fernandez | Juan Fernando Moreno | Jaime Gosalvez

Publisher:

Science Publications

Journal:

American Journal of Animal and Veterinary Sciences Vol.7 Issue 4, pp.180-185

Document Type:

Article

Publication Date:

12/2012

Document Language:

English

Document ID (Org No.):

2001396356

pISSN:

1557-4563

DOI:

10.3844/ajavsp.2012.180.185

Bacteria

DNA Fragmentation

Antibiotics

Issues

Article Details

Use of Quinolones in Bull Semen Extenders to Reduce Sperm Deoxyribonucleic Acid Damage

Authors: Clara Gonzalez-Marin | Michael Eduard Kjelland | Rosa Roy | Carmen Lopez-Fernandez | Jose Luis Fernandez | Juan Fernando Moreno | Jaime Gosalvez

Publisher: Science Publications

Journal: American Journal of Animal and Veterinary Sciences Vol.7 Issue 4, pp.180-185

Document Type: Article

Publication Date: 12/2012

Document Language: English

Document ID (Org No.): 2001396356

pISSN: 1557-4563

DOI: 10.3844/ajavsp.2012.180.185

Bacteria

DNA Fragmentation

Antibiotics

Issues

Authors:

Clara Gonzalez-Marin | Michael Eduard Kjelland | Rosa Roy | Carmen Lopez-Fernandez | Jose Luis Fernandez | Juan Fernando Moreno | Jaime Gosalvez

Publisher:

Science Publications

Journal:

American Journal of Animal and Veterinary Sciences Vol.7 Issue 4, pp.180-185

Document Type:

Article

Publication Date:

12/2012

Document Language:

English

Document ID (Org No.):

2001396356

pISSN:

1557-4563

DOI:

10.3844/ajavsp.2012.180.185