Evaluation of Wheat Landrace Germplasm for Agronomic Disease Susceptibility and Quality Traits Using Kompetitive Allele-Specific PCR (KASP) Markers

Alvina Gul; Peter John; Rabia Amir; Faiza Munir; Muhammad Jamil; Bengü Türkyılmaz Ünal; Münir Öztürk

Article Details

Evaluation of Wheat Landrace Germplasm for Agronomic Disease Susceptibility and Quality Traits Using Kompetitive Allele-Specific PCR (KASP) Markers

Authors:

Alvina Gul | Peter John | Rabia Amir | Faiza Munir | Muhammad Jamil | Bengü Türkyılmaz Ünal | Münir Öztürk

Publisher:

Ondokuz Mayıs University

Main Topics:

Tahıllar ve Yemeklik Tane Baklagiller

Journal:

Anadolu Tarım Bilimleri Dergisi Vol.40 Issue 2, pp.221-238

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001352187

pISSN:

1308-8750

Keywords:

Abstract:

 In the study, the genetic variation in agronomic, disease resistance and quality traits of seventy-three wheat landraces from Pakistan was analyzed by using 65 Kompetitive Allele-Specific PCR KASP assays. This research was intended to underpin the alleles for economically important genes in wheat landraces. Alleles, Rht-B1a and Rht-B1b were found in 64 and 30 of the population; while Rht-D1a and Rht-D1b were identified in 90 and 10 of the population respectively. Alleles responsible for flowering time Ppd-B1 were detected as sensitive in 46, and insensitive in 44 of the population while 56  and 39 insertion and deletions spanning 2Kb distance were observed in Ppd-D1. Similarly, 8, 15 and 2 assays were performed to study the genetic variation in vernalization, yield and drought tolerance. About 18 of the landraces were found with TaDREB_B1a allele which is drought tolerant. Unfortunately, more than eighty percent of wheat landraces were found to be susceptible to leaf rust confirmed with six assays. In addition, more than 90 of landraces were susceptible to stripe rust, stem rust and Fusarium head blight. Identified novel alleles from the current panel of wheat landraces can be used for marker-assisted as well genomic selection by breeding programs.

 In the study, the genetic variation in agronomic, disease resistance and quality traits of seventy-three wheat landraces from Pakistan was analyzed by using 65 Kompetitive Allele-Specific PCR KASP assays. This research was intended to underpin the alleles for economically important genes in wheat landraces. Alleles, Rht-B1a and Rht-B1b were found in 64 and 30 of the population; while Rht-D1a and Rht-D1b were identified in 90 and 10 of the population respectively. Alleles responsible for flowering time Ppd-B1 were detected as sensitive in 46, and insensitive in 44 of the population while 56  and 39 insertion and deletions spanning 2Kb distance were observed in Ppd-D1. Similarly, 8, 15 and 2 assays were performed to study the genetic variation in vernalization, yield and drought tolerance. About 18 of the landraces were found with TaDREB_B1a allele which is drought tolerant. Unfortunately, more than eighty percent of wheat landraces were found to be susceptible to leaf rust confirmed with six assays. In addition, more than 90 of landraces were susceptible to stripe rust, stem rust and Fusarium head blight. Identified novel alleles from the current panel of wheat landraces can be used for marker-assisted as well genomic selection by breeding programs.

 In the study, the genetic variation in agronomic, disease resistance and quality traits of seventy-three wheat landraces from Pakistan was analyzed by using 65 Kompetitive Allele-Specific PCR KASP assays. This research was intended to underpin the alleles for economically important genes in wheat landraces. Alleles, Rht-B1a and Rht-B1b were found in 64 and 30 of the population; while Rht-D1a and Rht-D1b were identified in 90 and 10 of the population respectively. Alleles responsible for flowering time Ppd-B1 were detected as sensitive in 46, and insensitive in 44 of the population while 56  and 39 insertion and deletions spanning 2Kb distance were observed in Ppd-D1. Similarly, 8, 15 and 2 assays were performed to study the genetic variation in vernalization, yield and drought tolerance. About 18 of the landraces were found with TaDREB_B1a allele which is drought tolerant. Unfortunately, more than eighty percent of wheat landraces were found to be susceptible to leaf rust confirmed with six assays. In addition, more than 90 of landraces were susceptible to stripe rust, stem rust and Fusarium head blight. Identified novel alleles from the current panel of wheat landraces can be used for marker-assisted as well genomic selection by breeding programs.

 Çalışmada, Pakistandaki yetmiş üç yerel buğday çeşidinin agronomik, hastalık direnci ve kalite özelliklerindeki genetik varyasyon, 65 Rekabetçi Alel Spesifik PCR KASP tahlili kullanılarak analiz edilmiştir. Bu araştırmanın amacı, yerel buğday çeşitlerinde ekonomik açıdan önemli genlere ilişkin alelleri belirlemektir. Aleller, Rht-B1a ve Rht-B1b popülasyonun 64 ve 30unda bulunmuştur; Rht-D1a ve Rht-D1b sırasıyla popülasyonun 90 ve 10unda tanımlanmıştır. Çiçeklenme zamanından sorumlu olan aleller Ppd-B1 popülasyonun 46sında duyarlı, 44ünde duyarsız olarak tespit edilirken, Ppd-D1de 2kb mesafeyi kapsayan 56 ve 39luk insersiyon ve delesyonlar gözlenmiştir. Benzer şekilde vernalizasyon, verim ve kuraklık toleransındaki genetik çeşitliliği incelemek için 8, 15 ve 2 tahlilleri yapılmıştır. Yerel çeşitlerde yaklaşık 18i kuraklığa dayanıklı TaDREB_B1a aleli ile bulunmuştur. Ne yazık ki, yerel buğday türlerinin yüzde sekseninden fazlasının yaprak pasına duyarlı olduğu altı testle doğrulanmıştır. Ek olarak, yerel türlerin 90ından fazlası şerit pası, gövde pası ve Fusarium baş yanıklığına karşı hassas olduğu tespit edilmiştir. Mevcut yerel buğday çeşitleri panelinden tanımlanan yeni aleller, ıslah programlarıyla genomik seçimin yanı sıra işaretleyici destekli olarak da kullanılabilir.

Article Details

Evaluation of Wheat Landrace Germplasm for Agronomic Disease Susceptibility and Quality Traits Using Kompetitive Allele-Specific PCR (KASP) Markers

Authors:

Alvina Gul | Peter John | Rabia Amir | Faiza Munir | Muhammad Jamil | Bengü Türkyılmaz Ünal | Münir Öztürk

Publisher:

Ondokuz Mayıs University

Main Topics:

Tahıllar ve Yemeklik Tane Baklagiller

Journal:

Anadolu Tarım Bilimleri Dergisi Vol.40 Issue 2, pp.221-238

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001352187

pISSN:

1308-8750

agronomic traits

disease resistance

drought tolerance

Triticum spp.tarımsal özellikler

hastalık direnci

kuraklığa tolerans

Triticum spp.

Issues

Article Details

Authors: Alvina Gul | Peter John | Rabia Amir | Faiza Munir | Muhammad Jamil | Bengü Türkyılmaz Ünal | Münir Öztürk

Publisher: Ondokuz Mayıs University

Main Topics: Tahıllar ve Yemeklik Tane Baklagiller

Journal: Anadolu Tarım Bilimleri Dergisi Vol.40 Issue 2, pp.221-238

Document Type: Article

Publication Date: 6/2025

Document Language: English

Document ID (Org No.): 2001352187

pISSN: 1308-8750

Issues

Authors:

Alvina Gul | Peter John | Rabia Amir | Faiza Munir | Muhammad Jamil | Bengü Türkyılmaz Ünal | Münir Öztürk

Publisher:

Ondokuz Mayıs University

Main Topics:

Tahıllar ve Yemeklik Tane Baklagiller

Journal:

Anadolu Tarım Bilimleri Dergisi Vol.40 Issue 2, pp.221-238

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001352187

pISSN:

1308-8750